Using Drosophila melanogaster to validate metabolism-based insecticide resistance from insect pests

Phillip J. Daborn; Christopher Lumb; Thomas W.R. Harrop; Alex Blasetti; Shivani Pasricha; Shai Morin; Sara N. Mitchell; Martin Donnelly; Pie Müller; Philip Batterham

doi:10.1016/j.ibmb.2012.09.003

Using Drosophila melanogaster to validate metabolism-based insecticide resistance from insect pests

Phillip J. Daborn
, Christopher Lumb
, Thomas W.R. Harrop
, Alex Blasetti
, Shivani Pasricha
, Shai Morin
, Sara N. Mitchell
, Martin Donnelly
, Pie Müller
, Philip Batterham

Vector Biology

Research output: Contribution to journal › Article › peer-review

81 Citations (Scopus)

Abstract

Identifying molecular mechanisms of insecticide resistance is important for preserving insecticide efficacy, developing new insecticides and implementing insect control. The metabolic detoxification of insecticides is a widespread resistance mechanism. Enzymes with the potential to detoxify insecticides are commonly encoded by members of the large cytochrome P450, glutathione S-transferase and carboxylesterase gene families, all rapidly evolving in insects. Here, we demonstrate that the model insect

Drosophila melanogaster is useful for functionally validating the role of metabolic enzymes in conferring

metabolism-based insecticide resistance. Alleles of three well-characterized genes from different pest insects were expressed in transgenic D. melanogaster : a carboxylesterase gene (aE7) from the Australian sheep blowfly Lucilia cuprina, a glutathione S-transferase gene (GstE2) from the mosquito Anopheles gambiae and a cytochrome P450 gene (Cyp6cm1) from the whitefly Bemisia tabaci. For all genes,expression in D. melanogaster resulted in insecticide resistance phenotypes mirroring those observed in resistant populations of the pest species. Using D. melanogaster to assess the potential for novel metabolic resistance mechanisms to evolve in pest species is discussed.

Original language	English
Pages (from-to)	918-924
Number of pages	7
Journal	Insect Biochemistry and Molecular Biology
Volume	42
Issue number	12
DOIs	https://doi.org/10.1016/j.ibmb.2012.09.003
Publication status	Published - 13 Sept 2012

Keywords

Carboxylesterase
Cytochrome P450
Glutathione S-transferase
Insecticide resistance

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10.1016/j.ibmb.2012.09.003

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title = "Using Drosophila melanogaster to validate metabolism-based insecticide resistance from insect pests",

abstract = "Identifying molecular mechanisms of insecticide resistance is important for preserving insecticide efficacy, developing new insecticides and implementing insect control. The metabolic detoxification of insecticides is a widespread resistance mechanism. Enzymes with the potential to detoxify insecticides are commonly encoded by members of the large cytochrome P450, glutathione S-transferase and carboxylesterase gene families, all rapidly evolving in insects. Here, we demonstrate that the model insectDrosophila melanogaster is useful for functionally validating the role of metabolic enzymes in conferringmetabolism-based insecticide resistance. Alleles of three well-characterized genes from different pest insects were expressed in transgenic D. melanogaster : a carboxylesterase gene (aE7) from the Australian sheep blowfly Lucilia cuprina, a glutathione S-transferase gene (GstE2) from the mosquito Anopheles gambiae and a cytochrome P450 gene (Cyp6cm1) from the whitefly Bemisia tabaci. For all genes,expression in D. melanogaster resulted in insecticide resistance phenotypes mirroring those observed in resistant populations of the pest species. Using D. melanogaster to assess the potential for novel metabolic resistance mechanisms to evolve in pest species is discussed.",

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AU - Pasricha, Shivani

AU - Morin, Shai

AU - Mitchell, Sara N.

AU - Donnelly, Martin

AU - Müller, Pie

AU - Batterham, Philip

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N2 - Identifying molecular mechanisms of insecticide resistance is important for preserving insecticide efficacy, developing new insecticides and implementing insect control. The metabolic detoxification of insecticides is a widespread resistance mechanism. Enzymes with the potential to detoxify insecticides are commonly encoded by members of the large cytochrome P450, glutathione S-transferase and carboxylesterase gene families, all rapidly evolving in insects. Here, we demonstrate that the model insectDrosophila melanogaster is useful for functionally validating the role of metabolic enzymes in conferringmetabolism-based insecticide resistance. Alleles of three well-characterized genes from different pest insects were expressed in transgenic D. melanogaster : a carboxylesterase gene (aE7) from the Australian sheep blowfly Lucilia cuprina, a glutathione S-transferase gene (GstE2) from the mosquito Anopheles gambiae and a cytochrome P450 gene (Cyp6cm1) from the whitefly Bemisia tabaci. For all genes,expression in D. melanogaster resulted in insecticide resistance phenotypes mirroring those observed in resistant populations of the pest species. Using D. melanogaster to assess the potential for novel metabolic resistance mechanisms to evolve in pest species is discussed.

AB - Identifying molecular mechanisms of insecticide resistance is important for preserving insecticide efficacy, developing new insecticides and implementing insect control. The metabolic detoxification of insecticides is a widespread resistance mechanism. Enzymes with the potential to detoxify insecticides are commonly encoded by members of the large cytochrome P450, glutathione S-transferase and carboxylesterase gene families, all rapidly evolving in insects. Here, we demonstrate that the model insectDrosophila melanogaster is useful for functionally validating the role of metabolic enzymes in conferringmetabolism-based insecticide resistance. Alleles of three well-characterized genes from different pest insects were expressed in transgenic D. melanogaster : a carboxylesterase gene (aE7) from the Australian sheep blowfly Lucilia cuprina, a glutathione S-transferase gene (GstE2) from the mosquito Anopheles gambiae and a cytochrome P450 gene (Cyp6cm1) from the whitefly Bemisia tabaci. For all genes,expression in D. melanogaster resulted in insecticide resistance phenotypes mirroring those observed in resistant populations of the pest species. Using D. melanogaster to assess the potential for novel metabolic resistance mechanisms to evolve in pest species is discussed.

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KW - Cytochrome P450

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Using Drosophila melanogaster to validate metabolism-based insecticide resistance from insect pests

Abstract

Keywords

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