Unusual norovirus and rotavirus genotypes in Ethiopia

Mohammed A. Yassin; Andrew Kirby; Abebayehu A. Mengistu; Isabel Arbide; Winifred Dove; Mandy Beyer; Nigel A. Cunliffe; Luis Cuevas

doi:10.1179/1465328111y.0000000047

Unusual norovirus and rotavirus genotypes in Ethiopia

Mohammed A. Yassin, Andrew Kirby, Abebayehu A. Mengistu, Isabel Arbide, Winifred Dove, Mandy Beyer, Nigel A. Cunliffe, Luis Cuevas

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

Background: Gastro-enteritis is associated with significant mortality in low- and middle-income countries, and rotavirus and norovirus are the principal viral agents implicated.

Aims: To investigate the molecular epidemiology and burden of disease associated with rotavirus and norovirus in children attending a health centre in Ethiopia.

Methods: A cross-sectional study was conducted in children attending a health centre in Awassa, southern Ethiopia. Children with diarrhoea (cases) and without diarrhoea (controls) were recruited over a 6-month period from December 2008 to May 2009. Rotavirus was detected by ELISA and genotyped by RT-PCR, and norovirus was amplified by RT-PCR with genotyping by sequence analysis of RT-PCR products.

Results: Rotavirus was detected in 44/200 (22%) cases and in none of the controls, with genotypes G3P[6] (48%), G1P[8] (27%) and G2P[4] (7%) being the strains most commonly identified. Norovirus was detected in 16 (8%) of 200 cases and in 4 (7%) of 57 controls. Norovirus GII.3 was the strain most commonly detected (40%: 8/20) and ten other genotypes were also detected.

Conclusion: Rotavirus is a common cause of severe gastro-enteritis in children in Ethiopia; however, norovirus occurred with a similar frequency in cases and controls. A globally uncommon strain type, G3P[6], predominated within the rotavirus strains detected.

Original language	English
Pages (from-to)	51-55
Number of pages	5
Journal	Paediatrics and International Child Health
Volume	32
Issue number	1
DOIs	https://doi.org/10.1179/1465328111y.0000000047
Publication status	Published - 1 Feb 2012

Keywords

Children
Diarrhoea
Ethiopia
Norovirus
Rotavirus

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Unusual norovirus and rotavirus genotypes in Ethiopia",

abstract = "Background: Gastro-enteritis is associated with significant mortality in low- and middle-income countries, and rotavirus and norovirus are the principal viral agents implicated.Aims: To investigate the molecular epidemiology and burden of disease associated with rotavirus and norovirus in children attending a health centre in Ethiopia.Methods: A cross-sectional study was conducted in children attending a health centre in Awassa, southern Ethiopia. Children with diarrhoea (cases) and without diarrhoea (controls) were recruited over a 6-month period from December 2008 to May 2009. Rotavirus was detected by ELISA and genotyped by RT-PCR, and norovirus was amplified by RT-PCR with genotyping by sequence analysis of RT-PCR products.Results: Rotavirus was detected in 44/200 (22\%) cases and in none of the controls, with genotypes G3P[6] (48\%), G1P[8] (27\%) and G2P[4] (7\%) being the strains most commonly identified. Norovirus was detected in 16 (8\%) of 200 cases and in 4 (7\%) of 57 controls. Norovirus GII.3 was the strain most commonly detected (40\%: 8/20) and ten other genotypes were also detected.Conclusion: Rotavirus is a common cause of severe gastro-enteritis in children in Ethiopia; however, norovirus occurred with a similar frequency in cases and controls. A globally uncommon strain type, G3P[6], predominated within the rotavirus strains detected.",

keywords = "Children, Diarrhoea, Ethiopia, Norovirus, Rotavirus",

author = "Yassin, \{Mohammed A.\} and Andrew Kirby and Mengistu, \{Abebayehu A.\} and Isabel Arbide and Winifred Dove and Mandy Beyer and Cunliffe, \{Nigel A.\} and Luis Cuevas",

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T1 - Unusual norovirus and rotavirus genotypes in Ethiopia

AU - Yassin, Mohammed A.

AU - Kirby, Andrew

AU - Mengistu, Abebayehu A.

AU - Arbide, Isabel

AU - Dove, Winifred

AU - Beyer, Mandy

AU - Cunliffe, Nigel A.

AU - Cuevas, Luis

PY - 2012/2/1

Y1 - 2012/2/1

N2 - Background: Gastro-enteritis is associated with significant mortality in low- and middle-income countries, and rotavirus and norovirus are the principal viral agents implicated.Aims: To investigate the molecular epidemiology and burden of disease associated with rotavirus and norovirus in children attending a health centre in Ethiopia.Methods: A cross-sectional study was conducted in children attending a health centre in Awassa, southern Ethiopia. Children with diarrhoea (cases) and without diarrhoea (controls) were recruited over a 6-month period from December 2008 to May 2009. Rotavirus was detected by ELISA and genotyped by RT-PCR, and norovirus was amplified by RT-PCR with genotyping by sequence analysis of RT-PCR products.Results: Rotavirus was detected in 44/200 (22%) cases and in none of the controls, with genotypes G3P[6] (48%), G1P[8] (27%) and G2P[4] (7%) being the strains most commonly identified. Norovirus was detected in 16 (8%) of 200 cases and in 4 (7%) of 57 controls. Norovirus GII.3 was the strain most commonly detected (40%: 8/20) and ten other genotypes were also detected.Conclusion: Rotavirus is a common cause of severe gastro-enteritis in children in Ethiopia; however, norovirus occurred with a similar frequency in cases and controls. A globally uncommon strain type, G3P[6], predominated within the rotavirus strains detected.

AB - Background: Gastro-enteritis is associated with significant mortality in low- and middle-income countries, and rotavirus and norovirus are the principal viral agents implicated.Aims: To investigate the molecular epidemiology and burden of disease associated with rotavirus and norovirus in children attending a health centre in Ethiopia.Methods: A cross-sectional study was conducted in children attending a health centre in Awassa, southern Ethiopia. Children with diarrhoea (cases) and without diarrhoea (controls) were recruited over a 6-month period from December 2008 to May 2009. Rotavirus was detected by ELISA and genotyped by RT-PCR, and norovirus was amplified by RT-PCR with genotyping by sequence analysis of RT-PCR products.Results: Rotavirus was detected in 44/200 (22%) cases and in none of the controls, with genotypes G3P[6] (48%), G1P[8] (27%) and G2P[4] (7%) being the strains most commonly identified. Norovirus was detected in 16 (8%) of 200 cases and in 4 (7%) of 57 controls. Norovirus GII.3 was the strain most commonly detected (40%: 8/20) and ten other genotypes were also detected.Conclusion: Rotavirus is a common cause of severe gastro-enteritis in children in Ethiopia; however, norovirus occurred with a similar frequency in cases and controls. A globally uncommon strain type, G3P[6], predominated within the rotavirus strains detected.

KW - Children

KW - Diarrhoea

KW - Ethiopia

KW - Norovirus

KW - Rotavirus

U2 - 10.1179/1465328111y.0000000047

DO - 10.1179/1465328111y.0000000047

M3 - Article

SN - 2046-9047

VL - 32

SP - 51

EP - 55

JO - Paediatrics and International Child Health

JF - Paediatrics and International Child Health

IS - 1

ER -

Unusual norovirus and rotavirus genotypes in Ethiopia

Abstract

Keywords

UN SDGs

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