Undiagnosed West Nile virus lineage 2d infection in a febrile patient from South-west Uganda, 2018.

Timothy Byaruhanga; Stuart Astbury; Jack D. Hill; Theocharis Tsoleridis; Joseph G. Chappell; John T. Kayiwa; Irene J. Ataliba; Annet M. Nankya; Jonathan Ball; Julius J. Lutwama; C. Patrick McClure

doi:10.1016/j.ijregi.2024.100462

Undiagnosed West Nile virus lineage 2d infection in a febrile patient from South-west Uganda, 2018.

Timothy Byaruhanga
, Stuart Astbury
, Jack D. Hill
, Theocharis Tsoleridis
, Joseph G. Chappell
, John T. Kayiwa
, Irene J. Ataliba
, Annet M. Nankya
, Jonathan Ball
, Julius J. Lutwama
, C. Patrick McClure

Tropical Disease Biology

University of Nottingham
Uganda Virus Research Institute
UK Department for Environment, Food and Rural Affairs
Nottingham University Hospitals NHS Trust
Pirbright Institute

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

We report the retrospective identification and subsequent recovery of a near-complete West Nile Virus lineage 2 genomes from a hospitalized patient with acute febrile illness in Uganda, using a combination of degenerate primer polymerase chain reaction screening and a novel 1200bp nanopore-based whole-genome amplicon sequencing scheme. This represents the first West Nile virus genome to be recovered from a human in Uganda since its discovery in 1937. Basic molecular rather than serological surveillance methods could be more widely deployed in the region to better diagnose febrile infections.

Original language	English
Article number	100462
Pages (from-to)	e100462
Journal	IJID Regions
Volume	13
Early online date	26 Sept 2024
DOIs	https://doi.org/10.1016/j.ijregi.2024.100462
Publication status	Published - 26 Sept 2024

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Acute febrile illness
Amplicon sequencing
Flavivirus
Orthoflavivirus
PCR
West Nile virus

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10.1016/j.ijregi.2024.100462Licence: CC BY-NC-ND

PMC11525451Final published version, 595 KBLicence: CC BY-NC-ND

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title = "Undiagnosed West Nile virus lineage 2d infection in a febrile patient from South-west Uganda, 2018.",

abstract = "We report the retrospective identification and subsequent recovery of a near-complete West Nile Virus lineage 2 genomes from a hospitalized patient with acute febrile illness in Uganda, using a combination of degenerate primer polymerase chain reaction screening and a novel 1200bp nanopore-based whole-genome amplicon sequencing scheme. This represents the first West Nile virus genome to be recovered from a human in Uganda since its discovery in 1937. Basic molecular rather than serological surveillance methods could be more widely deployed in the region to better diagnose febrile infections.",

keywords = "Acute febrile illness, Amplicon sequencing, Flavivirus, Orthoflavivirus, PCR, West Nile virus",

author = "Timothy Byaruhanga and Stuart Astbury and Hill, \{Jack D.\} and Theocharis Tsoleridis and Chappell, \{Joseph G.\} and Kayiwa, \{John T.\} and Ataliba, \{Irene J.\} and Nankya, \{Annet M.\} and Jonathan Ball and Lutwama, \{Julius J.\} and McClure, \{C. Patrick\}",

year = "2024",

month = sep,

day = "26",

doi = "10.1016/j.ijregi.2024.100462",

language = "English",

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journal = "IJID Regions",

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TY - JOUR

T1 - Undiagnosed West Nile virus lineage 2d infection in a febrile patient from South-west Uganda, 2018.

AU - Byaruhanga, Timothy

AU - Astbury, Stuart

AU - Hill, Jack D.

AU - Tsoleridis, Theocharis

AU - Chappell, Joseph G.

AU - Kayiwa, John T.

AU - Ataliba, Irene J.

AU - Nankya, Annet M.

AU - Ball, Jonathan

AU - Lutwama, Julius J.

AU - McClure, C. Patrick

PY - 2024/9/26

Y1 - 2024/9/26

N2 - We report the retrospective identification and subsequent recovery of a near-complete West Nile Virus lineage 2 genomes from a hospitalized patient with acute febrile illness in Uganda, using a combination of degenerate primer polymerase chain reaction screening and a novel 1200bp nanopore-based whole-genome amplicon sequencing scheme. This represents the first West Nile virus genome to be recovered from a human in Uganda since its discovery in 1937. Basic molecular rather than serological surveillance methods could be more widely deployed in the region to better diagnose febrile infections.

AB - We report the retrospective identification and subsequent recovery of a near-complete West Nile Virus lineage 2 genomes from a hospitalized patient with acute febrile illness in Uganda, using a combination of degenerate primer polymerase chain reaction screening and a novel 1200bp nanopore-based whole-genome amplicon sequencing scheme. This represents the first West Nile virus genome to be recovered from a human in Uganda since its discovery in 1937. Basic molecular rather than serological surveillance methods could be more widely deployed in the region to better diagnose febrile infections.

KW - Acute febrile illness

KW - Amplicon sequencing

KW - Flavivirus

KW - Orthoflavivirus

KW - PCR

KW - West Nile virus

U2 - 10.1016/j.ijregi.2024.100462

DO - 10.1016/j.ijregi.2024.100462

M3 - Article

SN - 2772-7076

VL - 13

SP - e100462

JO - IJID Regions

JF - IJID Regions

M1 - 100462

ER -

Undiagnosed West Nile virus lineage 2d infection in a febrile patient from South-west Uganda, 2018.

Abstract

UN SDGs

Keywords

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