Transfer of antibiotic resistance by transformation with eDNA within oral biofilms

Saad Hannan; Derren Ready; Azmiza S. Jasni; Michelle Rogers; Jonathan Pratten; Adam Roberts

doi:10.1111/j.1574-695x.2010.00661.x

Transfer of antibiotic resistance by transformation with eDNA within oral biofilms

Saad Hannan
, Derren Ready
, Azmiza S. Jasni
, Michelle Rogers
, Jonathan Pratten
, Adam Roberts

University College London

Research output: Contribution to journal › Article › peer-review

101 Citations (Scopus)

Abstract

We demonstrate that live donor Veillonella dispar cells can transfer the conjugative transposon Tn916 to four different Streptococcus spp. recipients in a multispecies oral consortium growing as a biofilm in a constant depth film fermentor. Additionally, we demonstrate that purified V. dispar DNA can transform Streptococcus mitis to tetracycline resistance in this experimental system. These data show that transfer of conjugative transposon-encoded antibiotic resistance can occur by transformation in addition to conjugation in biofilms.

Original language	English
Pages (from-to)	345-349
Number of pages	5
Journal	Pathogens and Disease
Volume	59
Issue number	3
DOIs	https://doi.org/10.1111/j.1574-695x.2010.00661.x
Publication status	Published - 1 Aug 2010
Externally published	Yes

Keywords

biofilm
conjugation
eDNA
horizontal gene transfer
Tn916
transformation

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10.1111/j.1574-695x.2010.00661.x

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title = "Transfer of antibiotic resistance by transformation with eDNA within oral biofilms",

abstract = "We demonstrate that live donor Veillonella dispar cells can transfer the conjugative transposon Tn916 to four different Streptococcus spp. recipients in a multispecies oral consortium growing as a biofilm in a constant depth film fermentor. Additionally, we demonstrate that purified V. dispar DNA can transform Streptococcus mitis to tetracycline resistance in this experimental system. These data show that transfer of conjugative transposon-encoded antibiotic resistance can occur by transformation in addition to conjugation in biofilms.",

keywords = "biofilm, conjugation, eDNA, horizontal gene transfer, Tn916, transformation",

author = "Saad Hannan and Derren Ready and Jasni, \{Azmiza S.\} and Michelle Rogers and Jonathan Pratten and Adam Roberts",

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AU - Hannan, Saad

AU - Ready, Derren

AU - Jasni, Azmiza S.

AU - Rogers, Michelle

AU - Pratten, Jonathan

AU - Roberts, Adam

PY - 2010/8/1

Y1 - 2010/8/1

N2 - We demonstrate that live donor Veillonella dispar cells can transfer the conjugative transposon Tn916 to four different Streptococcus spp. recipients in a multispecies oral consortium growing as a biofilm in a constant depth film fermentor. Additionally, we demonstrate that purified V. dispar DNA can transform Streptococcus mitis to tetracycline resistance in this experimental system. These data show that transfer of conjugative transposon-encoded antibiotic resistance can occur by transformation in addition to conjugation in biofilms.

AB - We demonstrate that live donor Veillonella dispar cells can transfer the conjugative transposon Tn916 to four different Streptococcus spp. recipients in a multispecies oral consortium growing as a biofilm in a constant depth film fermentor. Additionally, we demonstrate that purified V. dispar DNA can transform Streptococcus mitis to tetracycline resistance in this experimental system. These data show that transfer of conjugative transposon-encoded antibiotic resistance can occur by transformation in addition to conjugation in biofilms.

KW - biofilm

KW - conjugation

KW - eDNA

KW - horizontal gene transfer

KW - Tn916

KW - transformation

U2 - 10.1111/j.1574-695x.2010.00661.x

DO - 10.1111/j.1574-695x.2010.00661.x

M3 - Article

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VL - 59

SP - 345

EP - 349

JO - Pathogens and Disease

JF - Pathogens and Disease

IS - 3

ER -

Transfer of antibiotic resistance by transformation with eDNA within oral biofilms

Abstract

Keywords

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