The use of ELISA demonstrates the absence of Culex organophosphorus-resistance-associated esterases in Anopheles species

Janet Hemingway; Y. Rubio; K. E. Bobrowicz

doi:10.1016/0048-3575(86)90006-4

The use of ELISA demonstrates the absence of Culex organophosphorus-resistance-associated esterases in Anopheles species

Janet Hemingway, Y. Rubio, K. E. Bobrowicz

London School of Hygiene and Tropical Medicine

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

The esterase enzymes Est A′ and Est B are both quantitatively increased in organophosphorus-resistant Culex quinquefasciatus from Monrovia. An enzyme fraction containing Est B and traces of another unidentified esterase, but no Est A′, was produced by centrifugation and size exclusion chromatography. SDS-electrophoresis revealed five protein bands in this fraction. Antibodies raised in Balb-C mice to this fraction gave high levels of cross reactivity, in a peroxidase-linked ELISA test, to both organophosphorus-resistant and susceptible C. quinquefasciatus in fractions containing either Est B or Est A′. Only low levels of reactivity were obtained to a number of organophosphorus-resistant and susceptible Anopheles strains. This suggests that the family of esterases quantitatively changed in Culex are not present in the anophelines tested.

Original language	English
Pages (from-to)	327-335
Number of pages	9
Journal	Pesticide Biochemistry and Physiology
Volume	25
Issue number	3
DOIs	https://doi.org/10.1016/0048-3575(86)90006-4
Publication status	Published - 1 Jun 1986
Externally published	Yes

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title = "The use of ELISA demonstrates the absence of Culex organophosphorus-resistance-associated esterases in Anopheles species",

abstract = "The esterase enzymes Est A′ and Est B are both quantitatively increased in organophosphorus-resistant Culex quinquefasciatus from Monrovia. An enzyme fraction containing Est B and traces of another unidentified esterase, but no Est A′, was produced by centrifugation and size exclusion chromatography. SDS-electrophoresis revealed five protein bands in this fraction. Antibodies raised in Balb-C mice to this fraction gave high levels of cross reactivity, in a peroxidase-linked ELISA test, to both organophosphorus-resistant and susceptible C. quinquefasciatus in fractions containing either Est B or Est A′. Only low levels of reactivity were obtained to a number of organophosphorus-resistant and susceptible Anopheles strains. This suggests that the family of esterases quantitatively changed in Culex are not present in the anophelines tested.",

author = "Janet Hemingway and Y. Rubio and Bobrowicz, \{K. E.\}",

year = "1986",

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T1 - The use of ELISA demonstrates the absence of Culex organophosphorus-resistance-associated esterases in Anopheles species

AU - Hemingway, Janet

AU - Rubio, Y.

AU - Bobrowicz, K. E.

PY - 1986/6/1

Y1 - 1986/6/1

N2 - The esterase enzymes Est A′ and Est B are both quantitatively increased in organophosphorus-resistant Culex quinquefasciatus from Monrovia. An enzyme fraction containing Est B and traces of another unidentified esterase, but no Est A′, was produced by centrifugation and size exclusion chromatography. SDS-electrophoresis revealed five protein bands in this fraction. Antibodies raised in Balb-C mice to this fraction gave high levels of cross reactivity, in a peroxidase-linked ELISA test, to both organophosphorus-resistant and susceptible C. quinquefasciatus in fractions containing either Est B or Est A′. Only low levels of reactivity were obtained to a number of organophosphorus-resistant and susceptible Anopheles strains. This suggests that the family of esterases quantitatively changed in Culex are not present in the anophelines tested.

AB - The esterase enzymes Est A′ and Est B are both quantitatively increased in organophosphorus-resistant Culex quinquefasciatus from Monrovia. An enzyme fraction containing Est B and traces of another unidentified esterase, but no Est A′, was produced by centrifugation and size exclusion chromatography. SDS-electrophoresis revealed five protein bands in this fraction. Antibodies raised in Balb-C mice to this fraction gave high levels of cross reactivity, in a peroxidase-linked ELISA test, to both organophosphorus-resistant and susceptible C. quinquefasciatus in fractions containing either Est B or Est A′. Only low levels of reactivity were obtained to a number of organophosphorus-resistant and susceptible Anopheles strains. This suggests that the family of esterases quantitatively changed in Culex are not present in the anophelines tested.

U2 - 10.1016/0048-3575(86)90006-4

DO - 10.1016/0048-3575(86)90006-4

M3 - Article

SN - 0048-3575

VL - 25

SP - 327

EP - 335

JO - Pesticide Biochemistry and Physiology

JF - Pesticide Biochemistry and Physiology

IS - 3

ER -

The use of ELISA demonstrates the absence of Culex organophosphorus-resistance-associated esterases in Anopheles species

Abstract

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