The impact of pyrethroid-pyriproxyfen and pyrethroid-chlorfenapyr long-lasting insecticidal nets on density of primary malaria vectors Anopheles gambiae s.s. and Anopheles coluzzii in Benin: a secondary analysis of a cluster randomised controlled trial

Boulais Yovogan; Arthur Sovi; Armel Djènontin; Constantin J. Adoha; Bruno Akinro; Manfred Accrombessi; Edouard Dangbénon; Come Z. Koukpo; Zul Kifl Affolabi; Prudenciène A. Agboho; Casimir Dossou Kpanou; Landry Assongba; Antoine Abel Missihoun; Tatchémè Filémon Tokponnon; Clément Agbangla; Germain Gil Padonou; Louisa A. Messenger; Corine Ngufor; Jackie Cook; Martin C. Akogbéto; Natacha Protopopoff

doi:10.1186/s13071-023-06104-5

The impact of pyrethroid-pyriproxyfen and pyrethroid-chlorfenapyr long-lasting insecticidal nets on density of primary malaria vectors Anopheles gambiae s.s. and Anopheles coluzzii in Benin: a secondary analysis of a cluster randomised controlled trial

Boulais Yovogan
, Arthur Sovi
, Armel Djènontin
, Constantin J. Adoha
, Bruno Akinro
, Manfred Accrombessi
, Edouard Dangbénon
, Come Z. Koukpo
, Zul Kifl Affolabi
, Prudenciène A. Agboho
, Casimir Dossou Kpanou
, Landry Assongba
, Antoine Abel Missihoun
, Tatchémè Filémon Tokponnon
, Clément Agbangla
, Germain Gil Padonou
, Louisa A. Messenger
, Corine Ngufor
, Jackie Cook
, Martin C. Akogbéto

Natacha Protopopoff

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Background: Long-lasting insecticidal nets (LLINs) may have different impacts on distinct mosquito vector species. We assessed the efficacy of pyrethroid-pyriproxyfen and pyrethroid-chlorfenapyr LLINs on the density of Anopheles gambiae s.s. and An. coluzzii compared to pyrethroid-only nets in a three-arm cluster randomised control trial in Benin.

Methods: Indoor and outdoor collections of adult mosquitoes took place in 60 clusters using human landing catches at baseline and every 3 months for 2 years. After morphological identification, around 15% of randomly selected samples of An. gambiae s.l. were dissected to determine parity, species (using PCR). Results: Overall, a total of 46,613 mosquito specimens were collected at baseline and 259,250 in the eight quarterly collections post-net distribution. Post-net distribution, approximately 70% of the specimens of An. gambiae s.l. speciated were An. coluzzii, while the rest were mostly composed of An. gambiae s.s. with a small proportion (< 1%) of hybrids (An. gambiae/coluzzii). There was no evidence of a significant reduction in vector density indoors in either primary vector species [An. coluzzii: DR (density ratio) = 0.62 (95% CI 0.21–1.77), p = 0.3683 for the pyrethroid-pyriproxyfen LLIN and DR = 0.56 (95% CI 0.19–1.62), p = 0.2866 for the pyrethroid-chlorfenapyr LLIN, An. gambiae s.s.: DR = 0.52 (95% CI 0.18–1.46), p = 0.2192 for the pyrethroid-pyriproxyfen LLIN and DR = 0.53 (95% CI 0.19–1.46), p = 0.2222 for the pyrethroid-chlorfenapyr]. The same trend was observed outdoors. Parity rates of An. gambiae s.l. were also similar across study arms.

Conclusions: Compared with pyrethroid-only LLINs, pyrethroid-chlorfenapyr LLINs and pyrethroid-pyriproxyfen LLINs performed similarly against the two primary mosquito species An. gambiae s.s. and An. coluzzii in Benin.

Original language	English
Article number	7
Journal	Parasites and Vectors
Volume	17
Issue number	1
DOIs	https://doi.org/10.1186/s13071-023-06104-5
Publication status	Published - 4 Jan 2024
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Anopheles coluzzii
Anopheles gambiae s.s
Benin
Density
Dual active-ingredients LLINs

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Yovogan, B., Sovi, A., Djènontin, A., Adoha, C. J., Akinro, B., Accrombessi, M., Dangbénon, E., Koukpo, C. Z., Affolabi, Z. K., Agboho, P. A., Kpanou, C. D., Assongba, L., Missihoun, A. A., Tokponnon, T. F., Agbangla, C., Padonou, G. G., Messenger, L. A., Ngufor, C., Cook, J., ... Protopopoff, N. (2024). The impact of pyrethroid-pyriproxyfen and pyrethroid-chlorfenapyr long-lasting insecticidal nets on density of primary malaria vectors Anopheles gambiae s.s. and Anopheles coluzzii in Benin: a secondary analysis of a cluster randomised controlled trial. Parasites and Vectors, 17(1), Article 7. https://doi.org/10.1186/s13071-023-06104-5

@article{4de520b110f94ae9ac839408e97cb4a5,

title = "The impact of pyrethroid-pyriproxyfen and pyrethroid-chlorfenapyr long-lasting insecticidal nets on density of primary malaria vectors Anopheles gambiae s.s. and Anopheles coluzzii in Benin: a secondary analysis of a cluster randomised controlled trial",

abstract = "Background: Long-lasting insecticidal nets (LLINs) may have different impacts on distinct mosquito vector species. We assessed the efficacy of pyrethroid-pyriproxyfen and pyrethroid-chlorfenapyr LLINs on the density of Anopheles gambiae s.s. and An. coluzzii compared to pyrethroid-only nets in a three-arm cluster randomised control trial in Benin. Methods: Indoor and outdoor collections of adult mosquitoes took place in 60 clusters using human landing catches at baseline and every 3 months for 2 years. After morphological identification, around 15\% of randomly selected samples of An. gambiae s.l. were dissected to determine parity, species (using PCR). Results: Overall, a total of 46,613 mosquito specimens were collected at baseline and 259,250 in the eight quarterly collections post-net distribution. Post-net distribution, approximately 70\% of the specimens of An. gambiae s.l. speciated were An. coluzzii, while the rest were mostly composed of An. gambiae s.s. with a small proportion (< 1\%) of hybrids (An. gambiae/coluzzii). There was no evidence of a significant reduction in vector density indoors in either primary vector species [An. coluzzii: DR (density ratio) = 0.62 (95\% CI 0.21–1.77), p = 0.3683 for the pyrethroid-pyriproxyfen LLIN and DR = 0.56 (95\% CI 0.19–1.62), p = 0.2866 for the pyrethroid-chlorfenapyr LLIN, An. gambiae s.s.: DR = 0.52 (95\% CI 0.18–1.46), p = 0.2192 for the pyrethroid-pyriproxyfen LLIN and DR = 0.53 (95\% CI 0.19–1.46), p = 0.2222 for the pyrethroid-chlorfenapyr]. The same trend was observed outdoors. Parity rates of An. gambiae s.l. were also similar across study arms. Conclusions: Compared with pyrethroid-only LLINs, pyrethroid-chlorfenapyr LLINs and pyrethroid-pyriproxyfen LLINs performed similarly against the two primary mosquito species An. gambiae s.s. and An. coluzzii in Benin. ",

keywords = "Anopheles coluzzii, Anopheles gambiae s.s, Benin, Density, Dual active-ingredients LLINs",

author = "Boulais Yovogan and Arthur Sovi and Armel Dj{\`e}nontin and Adoha, \{Constantin J.\} and Bruno Akinro and Manfred Accrombessi and Edouard Dangb{\'e}non and Koukpo, \{Come Z.\} and Affolabi, \{Zul Kifl\} and Agboho, \{Prudenci{\`e}ne A.\} and Kpanou, \{Casimir Dossou\} and Landry Assongba and Missihoun, \{Antoine Abel\} and Tokponnon, \{Tatch{\'e}m{\`e} Fil{\'e}mon\} and Cl{\'e}ment Agbangla and Padonou, \{Germain Gil\} and Messenger, \{Louisa A.\} and Corine Ngufor and Jackie Cook and Akogb{\'e}to, \{Martin C.\} and Natacha Protopopoff",

year = "2024",

month = jan,

day = "4",

doi = "10.1186/s13071-023-06104-5",

language = "English",

volume = "17",

journal = "Parasites and Vectors",

issn = "1756-3305",

publisher = "BioMed Central Ltd",

number = "1",

}

Yovogan, B, Sovi, A, Djènontin, A, Adoha, CJ, Akinro, B, Accrombessi, M, Dangbénon, E, Koukpo, CZ, Affolabi, ZK, Agboho, PA, Kpanou, CD, Assongba, L, Missihoun, AA, Tokponnon, TF, Agbangla, C, Padonou, GG, Messenger, LA, Ngufor, C, Cook, J, Akogbéto, MC & Protopopoff, N 2024, 'The impact of pyrethroid-pyriproxyfen and pyrethroid-chlorfenapyr long-lasting insecticidal nets on density of primary malaria vectors Anopheles gambiae s.s. and Anopheles coluzzii in Benin: a secondary analysis of a cluster randomised controlled trial', Parasites and Vectors, vol. 17, no. 1, 7. https://doi.org/10.1186/s13071-023-06104-5

The impact of pyrethroid-pyriproxyfen and pyrethroid-chlorfenapyr long-lasting insecticidal nets on density of primary malaria vectors Anopheles gambiae s.s. and Anopheles coluzzii in Benin: a secondary analysis of a cluster randomised controlled trial. / Yovogan, Boulais; Sovi, Arthur; Djènontin, Armel et al.
In: Parasites and Vectors, Vol. 17, No. 1, 7, 04.01.2024.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - The impact of pyrethroid-pyriproxyfen and pyrethroid-chlorfenapyr long-lasting insecticidal nets on density of primary malaria vectors Anopheles gambiae s.s. and Anopheles coluzzii in Benin: a secondary analysis of a cluster randomised controlled trial

AU - Yovogan, Boulais

AU - Sovi, Arthur

AU - Djènontin, Armel

AU - Adoha, Constantin J.

AU - Akinro, Bruno

AU - Accrombessi, Manfred

AU - Dangbénon, Edouard

AU - Koukpo, Come Z.

AU - Affolabi, Zul Kifl

AU - Agboho, Prudenciène A.

AU - Kpanou, Casimir Dossou

AU - Assongba, Landry

AU - Missihoun, Antoine Abel

AU - Tokponnon, Tatchémè Filémon

AU - Agbangla, Clément

AU - Padonou, Germain Gil

AU - Messenger, Louisa A.

AU - Ngufor, Corine

AU - Cook, Jackie

AU - Akogbéto, Martin C.

AU - Protopopoff, Natacha

PY - 2024/1/4

Y1 - 2024/1/4

N2 - Background: Long-lasting insecticidal nets (LLINs) may have different impacts on distinct mosquito vector species. We assessed the efficacy of pyrethroid-pyriproxyfen and pyrethroid-chlorfenapyr LLINs on the density of Anopheles gambiae s.s. and An. coluzzii compared to pyrethroid-only nets in a three-arm cluster randomised control trial in Benin. Methods: Indoor and outdoor collections of adult mosquitoes took place in 60 clusters using human landing catches at baseline and every 3 months for 2 years. After morphological identification, around 15% of randomly selected samples of An. gambiae s.l. were dissected to determine parity, species (using PCR). Results: Overall, a total of 46,613 mosquito specimens were collected at baseline and 259,250 in the eight quarterly collections post-net distribution. Post-net distribution, approximately 70% of the specimens of An. gambiae s.l. speciated were An. coluzzii, while the rest were mostly composed of An. gambiae s.s. with a small proportion (< 1%) of hybrids (An. gambiae/coluzzii). There was no evidence of a significant reduction in vector density indoors in either primary vector species [An. coluzzii: DR (density ratio) = 0.62 (95% CI 0.21–1.77), p = 0.3683 for the pyrethroid-pyriproxyfen LLIN and DR = 0.56 (95% CI 0.19–1.62), p = 0.2866 for the pyrethroid-chlorfenapyr LLIN, An. gambiae s.s.: DR = 0.52 (95% CI 0.18–1.46), p = 0.2192 for the pyrethroid-pyriproxyfen LLIN and DR = 0.53 (95% CI 0.19–1.46), p = 0.2222 for the pyrethroid-chlorfenapyr]. The same trend was observed outdoors. Parity rates of An. gambiae s.l. were also similar across study arms. Conclusions: Compared with pyrethroid-only LLINs, pyrethroid-chlorfenapyr LLINs and pyrethroid-pyriproxyfen LLINs performed similarly against the two primary mosquito species An. gambiae s.s. and An. coluzzii in Benin.

AB - Background: Long-lasting insecticidal nets (LLINs) may have different impacts on distinct mosquito vector species. We assessed the efficacy of pyrethroid-pyriproxyfen and pyrethroid-chlorfenapyr LLINs on the density of Anopheles gambiae s.s. and An. coluzzii compared to pyrethroid-only nets in a three-arm cluster randomised control trial in Benin. Methods: Indoor and outdoor collections of adult mosquitoes took place in 60 clusters using human landing catches at baseline and every 3 months for 2 years. After morphological identification, around 15% of randomly selected samples of An. gambiae s.l. were dissected to determine parity, species (using PCR). Results: Overall, a total of 46,613 mosquito specimens were collected at baseline and 259,250 in the eight quarterly collections post-net distribution. Post-net distribution, approximately 70% of the specimens of An. gambiae s.l. speciated were An. coluzzii, while the rest were mostly composed of An. gambiae s.s. with a small proportion (< 1%) of hybrids (An. gambiae/coluzzii). There was no evidence of a significant reduction in vector density indoors in either primary vector species [An. coluzzii: DR (density ratio) = 0.62 (95% CI 0.21–1.77), p = 0.3683 for the pyrethroid-pyriproxyfen LLIN and DR = 0.56 (95% CI 0.19–1.62), p = 0.2866 for the pyrethroid-chlorfenapyr LLIN, An. gambiae s.s.: DR = 0.52 (95% CI 0.18–1.46), p = 0.2192 for the pyrethroid-pyriproxyfen LLIN and DR = 0.53 (95% CI 0.19–1.46), p = 0.2222 for the pyrethroid-chlorfenapyr]. The same trend was observed outdoors. Parity rates of An. gambiae s.l. were also similar across study arms. Conclusions: Compared with pyrethroid-only LLINs, pyrethroid-chlorfenapyr LLINs and pyrethroid-pyriproxyfen LLINs performed similarly against the two primary mosquito species An. gambiae s.s. and An. coluzzii in Benin.

KW - Anopheles coluzzii

KW - Anopheles gambiae s.s

KW - Benin

KW - Density

KW - Dual active-ingredients LLINs

U2 - 10.1186/s13071-023-06104-5

DO - 10.1186/s13071-023-06104-5

M3 - Article

C2 - 38178161

AN - SCOPUS:85181448519

SN - 1756-3305

VL - 17

JO - Parasites and Vectors

JF - Parasites and Vectors

IS - 1

M1 - 7

ER -

Yovogan B, Sovi A, Djènontin A, Adoha CJ, Akinro B, Accrombessi M et al. The impact of pyrethroid-pyriproxyfen and pyrethroid-chlorfenapyr long-lasting insecticidal nets on density of primary malaria vectors Anopheles gambiae s.s. and Anopheles coluzzii in Benin: a secondary analysis of a cluster randomised controlled trial. Parasites and Vectors. 2024 Jan 4;17(1):7. doi: 10.1186/s13071-023-06104-5