The humoral immune response of the spiny-tailed agamid lizard (Agama caudospinosum) to injection with Leishmania agamae promastigotes

George A. Ingram; David Molyneux

doi:10.1016/0165-2427(83)90008-9

The humoral immune response of the spiny-tailed agamid lizard (Agama caudospinosum) to injection with Leishmania agamae promastigotes

George A. Ingram, David Molyneux

University of Salford

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

Spiny-tailed agamid lizards (Agama caudospinosum) were given a single intraperitoneal injection of Leishmania agamae promastigotes. Direct agglutinins (DA), indirect haemagglutinins (IHA) and complement-fixing antibodies (CFA) produced against the parasites were non-precipitating, relatively thermostable and dithiothreitol sensitive. Antibodies were also detected by the immobilisation test (IMM) and by enzyme-linked immunosorbent assay (ELISA). The most sensitive method was the ELISA one. Antibodies were detected 7 days post-injection and maximum IMM (2-6), DA (2-8), CFA (2-10), IHA (2-12) and ELISA (2-16) titres were obtained from 35 to 49 days. In all cases, the levels of antibody following antigenic stimulation were significantly different from the controls (P<0.001). Serum lysozyme levels increased three-fold (P<0.001) with the highest value of 46 μg/ml occurring after 42 days.

Original language	English
Pages (from-to)	479-491
Number of pages	13
Journal	Veterinary Immunology and Immunopathology
Volume	4
Issue number	4
DOIs	https://doi.org/10.1016/0165-2427(83)90008-9
Publication status	Published - 1 May 1983
Externally published	Yes

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title = "The humoral immune response of the spiny-tailed agamid lizard (Agama caudospinosum) to injection with Leishmania agamae promastigotes",

abstract = "Spiny-tailed agamid lizards (Agama caudospinosum) were given a single intraperitoneal injection of Leishmania agamae promastigotes. Direct agglutinins (DA), indirect haemagglutinins (IHA) and complement-fixing antibodies (CFA) produced against the parasites were non-precipitating, relatively thermostable and dithiothreitol sensitive. Antibodies were also detected by the immobilisation test (IMM) and by enzyme-linked immunosorbent assay (ELISA). The most sensitive method was the ELISA one. Antibodies were detected 7 days post-injection and maximum IMM (2-6), DA (2-8), CFA (2-10), IHA (2-12) and ELISA (2-16) titres were obtained from 35 to 49 days. In all cases, the levels of antibody following antigenic stimulation were significantly different from the controls (P<0.001). Serum lysozyme levels increased three-fold (P<0.001) with the highest value of 46 μg/ml occurring after 42 days.",

author = "Ingram, \{George A.\} and David Molyneux",

year = "1983",

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T1 - The humoral immune response of the spiny-tailed agamid lizard (Agama caudospinosum) to injection with Leishmania agamae promastigotes

AU - Ingram, George A.

AU - Molyneux, David

PY - 1983/5/1

Y1 - 1983/5/1

N2 - Spiny-tailed agamid lizards (Agama caudospinosum) were given a single intraperitoneal injection of Leishmania agamae promastigotes. Direct agglutinins (DA), indirect haemagglutinins (IHA) and complement-fixing antibodies (CFA) produced against the parasites were non-precipitating, relatively thermostable and dithiothreitol sensitive. Antibodies were also detected by the immobilisation test (IMM) and by enzyme-linked immunosorbent assay (ELISA). The most sensitive method was the ELISA one. Antibodies were detected 7 days post-injection and maximum IMM (2-6), DA (2-8), CFA (2-10), IHA (2-12) and ELISA (2-16) titres were obtained from 35 to 49 days. In all cases, the levels of antibody following antigenic stimulation were significantly different from the controls (P<0.001). Serum lysozyme levels increased three-fold (P<0.001) with the highest value of 46 μg/ml occurring after 42 days.

AB - Spiny-tailed agamid lizards (Agama caudospinosum) were given a single intraperitoneal injection of Leishmania agamae promastigotes. Direct agglutinins (DA), indirect haemagglutinins (IHA) and complement-fixing antibodies (CFA) produced against the parasites were non-precipitating, relatively thermostable and dithiothreitol sensitive. Antibodies were also detected by the immobilisation test (IMM) and by enzyme-linked immunosorbent assay (ELISA). The most sensitive method was the ELISA one. Antibodies were detected 7 days post-injection and maximum IMM (2-6), DA (2-8), CFA (2-10), IHA (2-12) and ELISA (2-16) titres were obtained from 35 to 49 days. In all cases, the levels of antibody following antigenic stimulation were significantly different from the controls (P<0.001). Serum lysozyme levels increased three-fold (P<0.001) with the highest value of 46 μg/ml occurring after 42 days.

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DO - 10.1016/0165-2427(83)90008-9

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SP - 479

EP - 491

JO - Veterinary Immunology and Immunopathology

JF - Veterinary Immunology and Immunopathology

IS - 4

ER -

The humoral immune response of the spiny-tailed agamid lizard (Agama caudospinosum) to injection with Leishmania agamae promastigotes

Abstract

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