Standardized method for the study of antibody neutralization of HCV pseudoparticles (HCVpp)

Justin R. Bailey; Richard A. Urbanowicz; Jonathan Ball; Mansun Law; Steven K.H. Foung

doi:10.1007/978-1-4939-8976-8_30

Standardized method for the study of antibody neutralization of HCV pseudoparticles (HCVpp)

Justin R. Bailey
, Richard A. Urbanowicz
, Jonathan Ball
, Mansun Law
, Steven K.H. Foung

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

21 Citations (Scopus)

Abstract

Hepatitis C virus (HCV) pseudoparticles (HCVpp) are generated by cotransfection of HCV envelope (E1 and E2) genes along with a retroviral packaging/reporter construct into HEK293T cells. Enveloped particles bearing HCV E1E2 proteins on their surface are released through a retroviral budding process into the supernatant. Viral E1E2 glycoproteins facilitate a single round of receptor-mediated entry of HCVpp into hepatoma cells, which can be quantified by reporter gene expression. These HCVpp have been employed to study mechanisms of HCV entry into hepatoma cells, as well as HCV neutralization by immune sera or HCV-specific monoclonal antibodies.

Original language	English
Title of host publication	Methods in Molecular Biology
Pages	441-450
Number of pages	10
DOIs	https://doi.org/10.1007/978-1-4939-8976-8_30
Publication status	Published - 1 Jan 2019
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Flaviviridae
HCV pseudoparticles
Hepatitis C virus
Neutralizing antibodies
Neutralizing breadth

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10.1007/978-1-4939-8976-8_30

Cite this

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title = "Standardized method for the study of antibody neutralization of HCV pseudoparticles (HCVpp)",

abstract = "Hepatitis C virus (HCV) pseudoparticles (HCVpp) are generated by cotransfection of HCV envelope (E1 and E2) genes along with a retroviral packaging/reporter construct into HEK293T cells. Enveloped particles bearing HCV E1E2 proteins on their surface are released through a retroviral budding process into the supernatant. Viral E1E2 glycoproteins facilitate a single round of receptor-mediated entry of HCVpp into hepatoma cells, which can be quantified by reporter gene expression. These HCVpp have been employed to study mechanisms of HCV entry into hepatoma cells, as well as HCV neutralization by immune sera or HCV-specific monoclonal antibodies.",

keywords = "Flaviviridae, HCV pseudoparticles, Hepatitis C virus, Neutralizing antibodies, Neutralizing breadth",

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AU - Ball, Jonathan

AU - Law, Mansun

AU - Foung, Steven K.H.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Hepatitis C virus (HCV) pseudoparticles (HCVpp) are generated by cotransfection of HCV envelope (E1 and E2) genes along with a retroviral packaging/reporter construct into HEK293T cells. Enveloped particles bearing HCV E1E2 proteins on their surface are released through a retroviral budding process into the supernatant. Viral E1E2 glycoproteins facilitate a single round of receptor-mediated entry of HCVpp into hepatoma cells, which can be quantified by reporter gene expression. These HCVpp have been employed to study mechanisms of HCV entry into hepatoma cells, as well as HCV neutralization by immune sera or HCV-specific monoclonal antibodies.

AB - Hepatitis C virus (HCV) pseudoparticles (HCVpp) are generated by cotransfection of HCV envelope (E1 and E2) genes along with a retroviral packaging/reporter construct into HEK293T cells. Enveloped particles bearing HCV E1E2 proteins on their surface are released through a retroviral budding process into the supernatant. Viral E1E2 glycoproteins facilitate a single round of receptor-mediated entry of HCVpp into hepatoma cells, which can be quantified by reporter gene expression. These HCVpp have been employed to study mechanisms of HCV entry into hepatoma cells, as well as HCV neutralization by immune sera or HCV-specific monoclonal antibodies.

KW - Flaviviridae

KW - HCV pseudoparticles

KW - Hepatitis C virus

KW - Neutralizing antibodies

KW - Neutralizing breadth

U2 - 10.1007/978-1-4939-8976-8_30

DO - 10.1007/978-1-4939-8976-8_30

M3 - Chapter

SP - 441

EP - 450

BT - Methods in Molecular Biology

ER -

Standardized method for the study of antibody neutralization of HCV pseudoparticles (HCVpp)

Abstract

UN SDGs

Keywords

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Cite this