Single-Cell Profiling of Ebola Virus Disease In Vivo Reveals Viral and Host Dynamics

Dylan Kotliar; Aaron E. Lin; James Logue; Travis K. Hughes; Nadine M. Khoury; Siddharth S. Raju; Marc H. Wadsworth; Han Chen; Jonathan R. Kurtz; Bonnie Dighero-Kemp; Zach B. Bjornson; Nilanjan Mukherjee; Brian A. Sellers; Nancy Tran; Matthew R. Bauer; Gordon C. Adams; Ricky Adams; John L. Rinn; Marta Melé; Stephen F. Schaffner; Garry P. Nolan; Kayla Barnes; Lisa E. Hensley; David R. McIlwain; Alex K. Shalek; Pardis C. Sabeti; Richard S. Bennett

doi:10.1016/j.cell.2020.10.002

Single-Cell Profiling of Ebola Virus Disease In Vivo Reveals Viral and Host Dynamics

Dylan Kotliar
, Aaron E. Lin
, James Logue
, Travis K. Hughes
, Nadine M. Khoury
, Siddharth S. Raju
, Marc H. Wadsworth
, Han Chen
, Jonathan R. Kurtz
, Bonnie Dighero-Kemp
, Zach B. Bjornson
, Nilanjan Mukherjee
, Brian A. Sellers
, Nancy Tran
, Matthew R. Bauer
, Gordon C. Adams
, Ricky Adams
, John L. Rinn
, Marta Melé
, Stephen F. Schaffner

Garry P. Nolan, Kayla Barnes, Lisa E. Hensley, David R. McIlwain, Alex K. Shalek, Pardis C. Sabeti, Richard S. Bennett

Harvard University
Broad Institute
Massachusetts Institute of Technology
National Institutes of Health
Stanford University
University of Colorado Boulder
Barcelona Supercomputing Center
MRC-University of Glasgow Centre for Virus Research
Howard Hughes Medical Institute

Research output: Contribution to journal › Article › peer-review

99 Citations (Scopus)

Abstract

Single-cell profiling of circulating immune cells during Ebola virus (EBOV) infection in non-human primates resolves molecular correlates of viral tropism, characterizes replication dynamics within infected cells, and distinguishes expression changes that are mediated by viral infection from those due to cytokine signaling.

Original language	English
Pages (from-to)	1383-1401.e19
Journal	Cell
Volume	183
Issue number	5
DOIs	https://doi.org/10.1016/j.cell.2020.10.002
Publication status	Published - 25 Nov 2020
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

bystander cells
CyTOF
Ebola virus
host-virus interactions
interferon
monocytes
scRNA-Seq
Seq-Well
single-cell
viral tropism

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10.1016/j.cell.2020.10.002Licence: CC BY

Cite this

Kotliar, D., Lin, A. E., Logue, J., Hughes, T. K., Khoury, N. M., Raju, S. S., Wadsworth, M. H., Chen, H., Kurtz, J. R., Dighero-Kemp, B., Bjornson, Z. B., Mukherjee, N., Sellers, B. A., Tran, N., Bauer, M. R., Adams, G. C., Adams, R., Rinn, J. L., Melé, M., ... Bennett, R. S. (2020). Single-Cell Profiling of Ebola Virus Disease In Vivo Reveals Viral and Host Dynamics. Cell, 183(5), 1383-1401.e19. https://doi.org/10.1016/j.cell.2020.10.002

@article{dd2d8a3da5b4430e8ff43bbf1168390c,

title = "Single-Cell Profiling of Ebola Virus Disease In Vivo Reveals Viral and Host Dynamics",

abstract = "Single-cell profiling of circulating immune cells during Ebola virus (EBOV) infection in non-human primates resolves molecular correlates of viral tropism, characterizes replication dynamics within infected cells, and distinguishes expression changes that are mediated by viral infection from those due to cytokine signaling.",

keywords = "bystander cells, CyTOF, Ebola virus, host-virus interactions, interferon, monocytes, scRNA-Seq, Seq-Well, single-cell, viral tropism",

author = "Dylan Kotliar and Lin, \{Aaron E.\} and James Logue and Hughes, \{Travis K.\} and Khoury, \{Nadine M.\} and Raju, \{Siddharth S.\} and Wadsworth, \{Marc H.\} and Han Chen and Kurtz, \{Jonathan R.\} and Bonnie Dighero-Kemp and Bjornson, \{Zach B.\} and Nilanjan Mukherjee and Sellers, \{Brian A.\} and Nancy Tran and Bauer, \{Matthew R.\} and Adams, \{Gordon C.\} and Ricky Adams and Rinn, \{John L.\} and Marta Mel{\'e} and Schaffner, \{Stephen F.\} and Nolan, \{Garry P.\} and Kayla Barnes and Hensley, \{Lisa E.\} and McIlwain, \{David R.\} and Shalek, \{Alex K.\} and Sabeti, \{Pardis C.\} and Bennett, \{Richard S.\}",

year = "2020",

month = nov,

day = "25",

doi = "10.1016/j.cell.2020.10.002",

language = "English",

volume = "183",

pages = "1383--1401.e19",

journal = "Cell",

issn = "0092-8674",

publisher = "Elsevier B.V.",

number = "5",

}

Kotliar, D, Lin, AE, Logue, J, Hughes, TK, Khoury, NM, Raju, SS, Wadsworth, MH, Chen, H, Kurtz, JR, Dighero-Kemp, B, Bjornson, ZB, Mukherjee, N, Sellers, BA, Tran, N, Bauer, MR, Adams, GC, Adams, R, Rinn, JL, Melé, M, Schaffner, SF, Nolan, GP, Barnes, K, Hensley, LE, McIlwain, DR, Shalek, AK, Sabeti, PC & Bennett, RS 2020, 'Single-Cell Profiling of Ebola Virus Disease In Vivo Reveals Viral and Host Dynamics', Cell, vol. 183, no. 5, pp. 1383-1401.e19. https://doi.org/10.1016/j.cell.2020.10.002

TY - JOUR

T1 - Single-Cell Profiling of Ebola Virus Disease In Vivo Reveals Viral and Host Dynamics

AU - Kotliar, Dylan

AU - Lin, Aaron E.

AU - Logue, James

AU - Hughes, Travis K.

AU - Khoury, Nadine M.

AU - Raju, Siddharth S.

AU - Wadsworth, Marc H.

AU - Chen, Han

AU - Kurtz, Jonathan R.

AU - Dighero-Kemp, Bonnie

AU - Bjornson, Zach B.

AU - Mukherjee, Nilanjan

AU - Sellers, Brian A.

AU - Tran, Nancy

AU - Bauer, Matthew R.

AU - Adams, Gordon C.

AU - Adams, Ricky

AU - Rinn, John L.

AU - Melé, Marta

AU - Schaffner, Stephen F.

AU - Nolan, Garry P.

AU - Barnes, Kayla

AU - Hensley, Lisa E.

AU - McIlwain, David R.

AU - Shalek, Alex K.

AU - Sabeti, Pardis C.

AU - Bennett, Richard S.

PY - 2020/11/25

Y1 - 2020/11/25

N2 - Single-cell profiling of circulating immune cells during Ebola virus (EBOV) infection in non-human primates resolves molecular correlates of viral tropism, characterizes replication dynamics within infected cells, and distinguishes expression changes that are mediated by viral infection from those due to cytokine signaling.

AB - Single-cell profiling of circulating immune cells during Ebola virus (EBOV) infection in non-human primates resolves molecular correlates of viral tropism, characterizes replication dynamics within infected cells, and distinguishes expression changes that are mediated by viral infection from those due to cytokine signaling.

KW - bystander cells

KW - CyTOF

KW - Ebola virus

KW - host-virus interactions

KW - interferon

KW - monocytes

KW - scRNA-Seq

KW - Seq-Well

KW - single-cell

KW - viral tropism

U2 - 10.1016/j.cell.2020.10.002

DO - 10.1016/j.cell.2020.10.002

M3 - Article

SN - 0092-8674

VL - 183

SP - 1383-1401.e19

JO - Cell

JF - Cell

IS - 5

ER -

Single-Cell Profiling of Ebola Virus Disease In Vivo Reveals Viral and Host Dynamics

Abstract

UN SDGs

Keywords

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