Seroprevalence of Cytomegalovirus Among Pregnant Women at Kawempe National Referral Hospital, Uganda: A Cross-sectional Study: A Cross-sectional Study

Richard V. Katungye; Moses Musooko; Musa Sekikubo; Tobius Mutabazi; Mary Kyohere; Valerie Tusubira; Juliet Nsimire Sendagala; Joseph Peacock; Kirsty Le Doare; Annettee Nakimuli; Abdelmajid Djennad; Agnes Nyamaizi; Agnes Ssali; Alexander Amone; Amusa Wamawobe; Caitlin Farley; Carol Nanyunja; Christine Najuka; Cleophas Komugisha; Dan R. Shelley; Edward A.R. Portal; Ellie Duckworth; Emilie Karafillakis; Geraldine O'hara; Godfrey Matovu; Hannah G. Davies; Janet Seeley; Katie Cowie; Konstantinos Karampatsas; Lauren Hookham; Madeleine Cochet; Margaret Sewegaba; Maxensia Owor; Melanie Etti; Merryn Voysey; Owen B. Spiller; Patience Atuhaire; Paul T. Heath; Philippa Musoke; Phiona Nalubega; Pooja Ravji; Ritah Namugumya; Rosalin Parks; Rose Azuba; Sam Kipyeko; Simon Beach; Stephen Bentley; Tim Old; Vicki Chalker

doi:10.1093/ofid/ofae604

Seroprevalence of Cytomegalovirus Among Pregnant Women at Kawempe National Referral Hospital, Uganda: A Cross-sectional Study: A Cross-sectional Study

Richard V. Katungye, Moses Musooko, Musa Sekikubo, Tobius Mutabazi, Mary Kyohere, Valerie Tusubira, Juliet Nsimire Sendagala, Joseph Peacock, Kirsty Le Doare, Annettee Nakimuli, Abdelmajid Djennad, Agnes Nyamaizi, Agnes Ssali, Alexander Amone, Amusa Wamawobe, Caitlin Farley, Carol Nanyunja, Christine Najuka, Cleophas Komugisha, Dan R. ShelleyEdward A.R. Portal, Ellie Duckworth, Emilie Karafillakis, Geraldine O'hara, Godfrey Matovu, Hannah G. Davies, Janet Seeley, Katie Cowie, Konstantinos Karampatsas, Lauren Hookham, Madeleine Cochet, Margaret Sewegaba, Maxensia Owor, Melanie Etti, Merryn Voysey, Owen B. Spiller, Patience Atuhaire, Paul T. Heath, Philippa Musoke, Phiona Nalubega, Pooja Ravji, Ritah Namugumya, Rosalin Parks, Rose Azuba, Sam Kipyeko, Simon Beach, Stephen Bentley, Tim Old, Vicki Chalker

Liverpool School of Tropical Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Background: Maternal primary cytomegalovirus (CMV) infection is associated with abortion and congenital anomalies. In Uganda, the burden of maternal CMV infection is not well studied. This study thus assessed the seroprevalence and factors associated with CMV infection among pregnant women at Kawempe National Referral Hospital in Kampala. This work forms a part of the PROGRESS study, an observational cohort study undertaken in Kampala, Uganda, between November 2018 and April 2021. Methods: We conducted a cross-sectional study between September 2020 and January 2021 among the 639 pregnant women admitted to the labor ward at a government hospital. Sociodemographic, medical, obstetric, and socioeconomic data were collected. Blood samples from study participants were drawn and analyzed for the presence of CMV immunoglobulin G (IgG) and IgM using enzyme-linked immunosorbent assay-based quantitative assays. Further analysis of all IgM-positive samples was conducted using CMV IgG avidity assays. All infants had a nasal polymerase chain reaction (PCR) on the first day of life to investigate CMV positivity. Logistic regression was performed to determine the factors associated with CMV infection. Results: Seroprevalence of CMV IgG among the 637 women was universal (100%), and that of CMV (IgM) was 5.8% (37/637). CMV (IgM) was associated with being low socioeconomic status (odds ratio, 3.44; 95% CI, 1.05-11.32; P =. 04). Transmission risk was low, and no infant had a positive PCR for CMV at birth. Conclusions: Universally, by the time women in Kampala conceive, they will have been exposed to CMV. Women of lower socioeconomic status were more likely to have recent CMV infection than their more affluent counterparts, highlighting the need for screening guidelines in this setting.

Original language	English
Pages (from-to)	S200-S205
Journal	Open Forum Infectious Diseases
Volume	11
DOIs	https://doi.org/10.1093/ofid/ofae604
Publication status	Published - 1 Dec 2024

Keywords

CMV
infants
pregnant women
seropositivity
Uganda

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Katungye, R. V., Musooko, M., Sekikubo, M., Mutabazi, T., Kyohere, M., Tusubira, V., Sendagala, J. N., Peacock, J., Le Doare, K., Nakimuli, A., Djennad, A., Nyamaizi, A., Ssali, A., Amone, A., Wamawobe, A., Farley, C., Nanyunja, C., Najuka, C., Komugisha, C., ... Chalker, V. (2024). Seroprevalence of Cytomegalovirus Among Pregnant Women at Kawempe National Referral Hospital, Uganda: A Cross-sectional Study: A Cross-sectional Study. Open Forum Infectious Diseases, 11, S200-S205. https://doi.org/10.1093/ofid/ofae604

@article{b0c3fe325aa54db0867b957ab163895c,

title = "Seroprevalence of Cytomegalovirus Among Pregnant Women at Kawempe National Referral Hospital, Uganda: A Cross-sectional Study: A Cross-sectional Study",

abstract = "Background: Maternal primary cytomegalovirus (CMV) infection is associated with abortion and congenital anomalies. In Uganda, the burden of maternal CMV infection is not well studied. This study thus assessed the seroprevalence and factors associated with CMV infection among pregnant women at Kawempe National Referral Hospital in Kampala. This work forms a part of the PROGRESS study, an observational cohort study undertaken in Kampala, Uganda, between November 2018 and April 2021. Methods: We conducted a cross-sectional study between September 2020 and January 2021 among the 639 pregnant women admitted to the labor ward at a government hospital. Sociodemographic, medical, obstetric, and socioeconomic data were collected. Blood samples from study participants were drawn and analyzed for the presence of CMV immunoglobulin G (IgG) and IgM using enzyme-linked immunosorbent assay-based quantitative assays. Further analysis of all IgM-positive samples was conducted using CMV IgG avidity assays. All infants had a nasal polymerase chain reaction (PCR) on the first day of life to investigate CMV positivity. Logistic regression was performed to determine the factors associated with CMV infection. Results: Seroprevalence of CMV IgG among the 637 women was universal (100\%), and that of CMV (IgM) was 5.8\% (37/637). CMV (IgM) was associated with being low socioeconomic status (odds ratio, 3.44; 95\% CI, 1.05-11.32; P =. 04). Transmission risk was low, and no infant had a positive PCR for CMV at birth. Conclusions: Universally, by the time women in Kampala conceive, they will have been exposed to CMV. Women of lower socioeconomic status were more likely to have recent CMV infection than their more affluent counterparts, highlighting the need for screening guidelines in this setting.",

keywords = "CMV, infants, pregnant women, seropositivity, Uganda",

author = "Katungye, \{Richard V.\} and Moses Musooko and Musa Sekikubo and Tobius Mutabazi and Mary Kyohere and Valerie Tusubira and Sendagala, \{Juliet Nsimire\} and Joseph Peacock and \{Le Doare\}, Kirsty and Annettee Nakimuli and Abdelmajid Djennad and Agnes Nyamaizi and Agnes Ssali and Alexander Amone and Amusa Wamawobe and Caitlin Farley and Carol Nanyunja and Christine Najuka and Cleophas Komugisha and Shelley, \{Dan R.\} and Portal, \{Edward A.R.\} and Ellie Duckworth and Emilie Karafillakis and Geraldine O'hara and Godfrey Matovu and Davies, \{Hannah G.\} and Janet Seeley and Katie Cowie and Konstantinos Karampatsas and Lauren Hookham and Madeleine Cochet and Margaret Sewegaba and Maxensia Owor and Melanie Etti and Merryn Voysey and Spiller, \{Owen B.\} and Patience Atuhaire and Heath, \{Paul T.\} and Philippa Musoke and Phiona Nalubega and Pooja Ravji and Ritah Namugumya and Rosalin Parks and Rose Azuba and Sam Kipyeko and Simon Beach and Stephen Bentley and Tim Old and Vicki Chalker",

year = "2024",

month = dec,

day = "1",

doi = "10.1093/ofid/ofae604",

language = "English",

volume = "11",

pages = "S200--S205",

journal = "Open Forum Infectious Diseases",

issn = "2328-8957",

publisher = "Oxford University Press",

}

Katungye, RV, Musooko, M, Sekikubo, M, Mutabazi, T, Kyohere, M, Tusubira, V, Sendagala, JN, Peacock, J, Le Doare, K, Nakimuli, A, Djennad, A, Nyamaizi, A, Ssali, A, Amone, A, Wamawobe, A, Farley, C, Nanyunja, C, Najuka, C, Komugisha, C, Shelley, DR, Portal, EAR, Duckworth, E, Karafillakis, E, O'hara, G, Matovu, G, Davies, HG, Seeley, J, Cowie, K, Karampatsas, K, Hookham, L, Cochet, M, Sewegaba, M, Owor, M, Etti, M, Voysey, M, Spiller, OB, Atuhaire, P, Heath, PT, Musoke, P, Nalubega, P, Ravji, P, Namugumya, R, Parks, R, Azuba, R, Kipyeko, S, Beach, S, Bentley, S, Old, T & Chalker, V 2024, 'Seroprevalence of Cytomegalovirus Among Pregnant Women at Kawempe National Referral Hospital, Uganda: A Cross-sectional Study: A Cross-sectional Study', Open Forum Infectious Diseases, vol. 11, pp. S200-S205. https://doi.org/10.1093/ofid/ofae604

TY - JOUR

T1 - Seroprevalence of Cytomegalovirus Among Pregnant Women at Kawempe National Referral Hospital, Uganda: A Cross-sectional Study: A Cross-sectional Study

AU - Katungye, Richard V.

AU - Musooko, Moses

AU - Sekikubo, Musa

AU - Mutabazi, Tobius

AU - Kyohere, Mary

AU - Tusubira, Valerie

AU - Sendagala, Juliet Nsimire

AU - Peacock, Joseph

AU - Le Doare, Kirsty

AU - Nakimuli, Annettee

AU - Djennad, Abdelmajid

AU - Nyamaizi, Agnes

AU - Ssali, Agnes

AU - Amone, Alexander

AU - Wamawobe, Amusa

AU - Farley, Caitlin

AU - Nanyunja, Carol

AU - Najuka, Christine

AU - Komugisha, Cleophas

AU - Shelley, Dan R.

AU - Portal, Edward A.R.

AU - Duckworth, Ellie

AU - Karafillakis, Emilie

AU - O'hara, Geraldine

AU - Matovu, Godfrey

AU - Davies, Hannah G.

AU - Seeley, Janet

AU - Cowie, Katie

AU - Karampatsas, Konstantinos

AU - Hookham, Lauren

AU - Cochet, Madeleine

AU - Sewegaba, Margaret

AU - Owor, Maxensia

AU - Etti, Melanie

AU - Voysey, Merryn

AU - Spiller, Owen B.

AU - Atuhaire, Patience

AU - Heath, Paul T.

AU - Musoke, Philippa

AU - Nalubega, Phiona

AU - Ravji, Pooja

AU - Namugumya, Ritah

AU - Parks, Rosalin

AU - Azuba, Rose

AU - Kipyeko, Sam

AU - Beach, Simon

AU - Bentley, Stephen

AU - Old, Tim

AU - Chalker, Vicki

PY - 2024/12/1

Y1 - 2024/12/1

N2 - Background: Maternal primary cytomegalovirus (CMV) infection is associated with abortion and congenital anomalies. In Uganda, the burden of maternal CMV infection is not well studied. This study thus assessed the seroprevalence and factors associated with CMV infection among pregnant women at Kawempe National Referral Hospital in Kampala. This work forms a part of the PROGRESS study, an observational cohort study undertaken in Kampala, Uganda, between November 2018 and April 2021. Methods: We conducted a cross-sectional study between September 2020 and January 2021 among the 639 pregnant women admitted to the labor ward at a government hospital. Sociodemographic, medical, obstetric, and socioeconomic data were collected. Blood samples from study participants were drawn and analyzed for the presence of CMV immunoglobulin G (IgG) and IgM using enzyme-linked immunosorbent assay-based quantitative assays. Further analysis of all IgM-positive samples was conducted using CMV IgG avidity assays. All infants had a nasal polymerase chain reaction (PCR) on the first day of life to investigate CMV positivity. Logistic regression was performed to determine the factors associated with CMV infection. Results: Seroprevalence of CMV IgG among the 637 women was universal (100%), and that of CMV (IgM) was 5.8% (37/637). CMV (IgM) was associated with being low socioeconomic status (odds ratio, 3.44; 95% CI, 1.05-11.32; P =. 04). Transmission risk was low, and no infant had a positive PCR for CMV at birth. Conclusions: Universally, by the time women in Kampala conceive, they will have been exposed to CMV. Women of lower socioeconomic status were more likely to have recent CMV infection than their more affluent counterparts, highlighting the need for screening guidelines in this setting.

AB - Background: Maternal primary cytomegalovirus (CMV) infection is associated with abortion and congenital anomalies. In Uganda, the burden of maternal CMV infection is not well studied. This study thus assessed the seroprevalence and factors associated with CMV infection among pregnant women at Kawempe National Referral Hospital in Kampala. This work forms a part of the PROGRESS study, an observational cohort study undertaken in Kampala, Uganda, between November 2018 and April 2021. Methods: We conducted a cross-sectional study between September 2020 and January 2021 among the 639 pregnant women admitted to the labor ward at a government hospital. Sociodemographic, medical, obstetric, and socioeconomic data were collected. Blood samples from study participants were drawn and analyzed for the presence of CMV immunoglobulin G (IgG) and IgM using enzyme-linked immunosorbent assay-based quantitative assays. Further analysis of all IgM-positive samples was conducted using CMV IgG avidity assays. All infants had a nasal polymerase chain reaction (PCR) on the first day of life to investigate CMV positivity. Logistic regression was performed to determine the factors associated with CMV infection. Results: Seroprevalence of CMV IgG among the 637 women was universal (100%), and that of CMV (IgM) was 5.8% (37/637). CMV (IgM) was associated with being low socioeconomic status (odds ratio, 3.44; 95% CI, 1.05-11.32; P =. 04). Transmission risk was low, and no infant had a positive PCR for CMV at birth. Conclusions: Universally, by the time women in Kampala conceive, they will have been exposed to CMV. Women of lower socioeconomic status were more likely to have recent CMV infection than their more affluent counterparts, highlighting the need for screening guidelines in this setting.

KW - CMV

KW - infants

KW - pregnant women

KW - seropositivity

KW - Uganda

U2 - 10.1093/ofid/ofae604

DO - 10.1093/ofid/ofae604

M3 - Article

SN - 2328-8957

VL - 11

SP - S200-S205

JO - Open Forum Infectious Diseases

JF - Open Forum Infectious Diseases

ER -

Seroprevalence of Cytomegalovirus Among Pregnant Women at Kawempe National Referral Hospital, Uganda: A Cross-sectional Study: A Cross-sectional Study

Abstract

Keywords

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