Serological evidence of Zika virus infection in non-human primates in Zambia

Christida Wastika; Michihito Sasaki; Kentaro Yoshii; Paulina D. Anindita; Bernard M. Hang’ombe; Aaron S. Mweene; Shintaro Kobayashi; Hiroaki Kariwa; Michael J. Carr; William W. Hall; Yuki Eshita; Yasuko Orba; Hirofumi Sawa

doi:10.1007/s00705-019-04302-0

Serological evidence of Zika virus infection in non-human primates in Zambia

Christida Wastika, Michihito Sasaki, Kentaro Yoshii, Paulina D. Anindita, Bernard M. Hang’ombe, Aaron S. Mweene, Shintaro Kobayashi, Hiroaki Kariwa, Michael J. Carr, William W. Hall, Yuki Eshita, Yasuko Orba, Hirofumi Sawa

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Abstract

Zika virus (ZIKV) circulation occurs between non-human primates (NHPs) in a sylvatic transmission cycle. To investigate evidence of flavivirus infection in NHPs in Zambia, we performed a plaque reduction neutralization test (PRNT) to quantify neutralizing antibodies. PRNT revealed that sera from NHPs (African green monkeys and baboons) exhibited neutralizing activity against ZIKV (34.4%; 33/96), whereas a PRNT for yellow fever virus using NHP sera showed no neutralization activity. ZIKV genomic RNA was not detected in splenic tissues from NHPs, suggesting that the presence of anti-ZIKV neutralizing antibodies represented resolved infections. Our evidence suggests that ZIKV is maintained in NHP reservoirs in Zambia.

Original language	English
Pages (from-to)	2165-2170
Number of pages	6
Journal	Archives of Virology
Volume	164
Issue number	8
DOIs	https://doi.org/10.1007/s00705-019-04302-0
Publication status	Published - 1 Aug 2019
Externally published	Yes

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Cite this

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title = "Serological evidence of Zika virus infection in non-human primates in Zambia",

abstract = "Zika virus (ZIKV) circulation occurs between non-human primates (NHPs) in a sylvatic transmission cycle. To investigate evidence of flavivirus infection in NHPs in Zambia, we performed a plaque reduction neutralization test (PRNT) to quantify neutralizing antibodies. PRNT revealed that sera from NHPs (African green monkeys and baboons) exhibited neutralizing activity against ZIKV (34.4\%; 33/96), whereas a PRNT for yellow fever virus using NHP sera showed no neutralization activity. ZIKV genomic RNA was not detected in splenic tissues from NHPs, suggesting that the presence of anti-ZIKV neutralizing antibodies represented resolved infections. Our evidence suggests that ZIKV is maintained in NHP reservoirs in Zambia.",

author = "Christida Wastika and Michihito Sasaki and Kentaro Yoshii and Anindita, \{Paulina D.\} and Hang{\textquoteright}ombe, \{Bernard M.\} and Mweene, \{Aaron S.\} and Shintaro Kobayashi and Hiroaki Kariwa and Carr, \{Michael J.\} and Hall, \{William W.\} and Yuki Eshita and Yasuko Orba and Hirofumi Sawa",

year = "2019",

month = aug,

day = "1",

doi = "10.1007/s00705-019-04302-0",

language = "English",

volume = "164",

pages = "2165--2170",

journal = "Archives of Virology",

issn = "0304-8608",

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number = "8",

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TY - JOUR

T1 - Serological evidence of Zika virus infection in non-human primates in Zambia

AU - Wastika, Christida

AU - Sasaki, Michihito

AU - Yoshii, Kentaro

AU - Anindita, Paulina D.

AU - Hang’ombe, Bernard M.

AU - Mweene, Aaron S.

AU - Kobayashi, Shintaro

AU - Kariwa, Hiroaki

AU - Carr, Michael J.

AU - Hall, William W.

AU - Eshita, Yuki

AU - Orba, Yasuko

AU - Sawa, Hirofumi

PY - 2019/8/1

Y1 - 2019/8/1

N2 - Zika virus (ZIKV) circulation occurs between non-human primates (NHPs) in a sylvatic transmission cycle. To investigate evidence of flavivirus infection in NHPs in Zambia, we performed a plaque reduction neutralization test (PRNT) to quantify neutralizing antibodies. PRNT revealed that sera from NHPs (African green monkeys and baboons) exhibited neutralizing activity against ZIKV (34.4%; 33/96), whereas a PRNT for yellow fever virus using NHP sera showed no neutralization activity. ZIKV genomic RNA was not detected in splenic tissues from NHPs, suggesting that the presence of anti-ZIKV neutralizing antibodies represented resolved infections. Our evidence suggests that ZIKV is maintained in NHP reservoirs in Zambia.

AB - Zika virus (ZIKV) circulation occurs between non-human primates (NHPs) in a sylvatic transmission cycle. To investigate evidence of flavivirus infection in NHPs in Zambia, we performed a plaque reduction neutralization test (PRNT) to quantify neutralizing antibodies. PRNT revealed that sera from NHPs (African green monkeys and baboons) exhibited neutralizing activity against ZIKV (34.4%; 33/96), whereas a PRNT for yellow fever virus using NHP sera showed no neutralization activity. ZIKV genomic RNA was not detected in splenic tissues from NHPs, suggesting that the presence of anti-ZIKV neutralizing antibodies represented resolved infections. Our evidence suggests that ZIKV is maintained in NHP reservoirs in Zambia.

U2 - 10.1007/s00705-019-04302-0

DO - 10.1007/s00705-019-04302-0

M3 - Article

SN - 0304-8608

VL - 164

SP - 2165

EP - 2170

JO - Archives of Virology

JF - Archives of Virology

IS - 8

ER -

Serological evidence of Zika virus infection in non-human primates in Zambia

Abstract

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