Serological evidence of Zika virus infection in non-human primates in Zambia

Christida Wastika; Michihito Sasaki; Kentaro Yoshii; Paulina D. Anindita; Bernard M. Hang’ombe; Aaron S. Mweene; Shintaro Kobayashi; Hiroaki Kariwa; Michael J. Carr; William W. Hall; Yuki Eshita; Yasuko Orba; Hirofumi Sawa

doi:10.1007/s00705-019-04302-0

Serological evidence of Zika virus infection in non-human primates in Zambia

Christida Wastika
, Michihito Sasaki
, Kentaro Yoshii
, Paulina D. Anindita
, Bernard M. Hang’ombe
, Aaron S. Mweene
, Shintaro Kobayashi
, Hiroaki Kariwa
, Michael J. Carr
, William W. Hall
, Yuki Eshita
, Yasuko Orba
, Hirofumi Sawa

Hokkaido University
University of Zambia
Africa Centre of Excellence for Infectious Diseases of Humans and Animals
University College Dublin
Global Virus Network

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

Zika virus (ZIKV) circulation occurs between non-human primates (NHPs) in a sylvatic transmission cycle. To investigate evidence of flavivirus infection in NHPs in Zambia, we performed a plaque reduction neutralization test (PRNT) to quantify neutralizing antibodies. PRNT revealed that sera from NHPs (African green monkeys and baboons) exhibited neutralizing activity against ZIKV (34.4%; 33/96), whereas a PRNT for yellow fever virus using NHP sera showed no neutralization activity. ZIKV genomic RNA was not detected in splenic tissues from NHPs, suggesting that the presence of anti-ZIKV neutralizing antibodies represented resolved infections. Our evidence suggests that ZIKV is maintained in NHP reservoirs in Zambia.

Original language	English
Pages (from-to)	2165-2170
Number of pages	6
Journal	Archives of Virology
Volume	164
Issue number	8
DOIs	https://doi.org/10.1007/s00705-019-04302-0
Publication status	Published - 1 Aug 2019
Externally published	Yes

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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10.1007/s00705-019-04302-0

Cite this

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title = "Serological evidence of Zika virus infection in non-human primates in Zambia",

abstract = "Zika virus (ZIKV) circulation occurs between non-human primates (NHPs) in a sylvatic transmission cycle. To investigate evidence of flavivirus infection in NHPs in Zambia, we performed a plaque reduction neutralization test (PRNT) to quantify neutralizing antibodies. PRNT revealed that sera from NHPs (African green monkeys and baboons) exhibited neutralizing activity against ZIKV (34.4\%; 33/96), whereas a PRNT for yellow fever virus using NHP sera showed no neutralization activity. ZIKV genomic RNA was not detected in splenic tissues from NHPs, suggesting that the presence of anti-ZIKV neutralizing antibodies represented resolved infections. Our evidence suggests that ZIKV is maintained in NHP reservoirs in Zambia.",

author = "Christida Wastika and Michihito Sasaki and Kentaro Yoshii and Anindita, \{Paulina D.\} and Hang{\textquoteright}ombe, \{Bernard M.\} and Mweene, \{Aaron S.\} and Shintaro Kobayashi and Hiroaki Kariwa and Carr, \{Michael J.\} and Hall, \{William W.\} and Yuki Eshita and Yasuko Orba and Hirofumi Sawa",

year = "2019",

month = aug,

day = "1",

doi = "10.1007/s00705-019-04302-0",

language = "English",

volume = "164",

pages = "2165--2170",

journal = "Archives of Virology",

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TY - JOUR

T1 - Serological evidence of Zika virus infection in non-human primates in Zambia

AU - Wastika, Christida

AU - Sasaki, Michihito

AU - Yoshii, Kentaro

AU - Anindita, Paulina D.

AU - Hang’ombe, Bernard M.

AU - Mweene, Aaron S.

AU - Kobayashi, Shintaro

AU - Kariwa, Hiroaki

AU - Carr, Michael J.

AU - Hall, William W.

AU - Eshita, Yuki

AU - Orba, Yasuko

AU - Sawa, Hirofumi

PY - 2019/8/1

Y1 - 2019/8/1

N2 - Zika virus (ZIKV) circulation occurs between non-human primates (NHPs) in a sylvatic transmission cycle. To investigate evidence of flavivirus infection in NHPs in Zambia, we performed a plaque reduction neutralization test (PRNT) to quantify neutralizing antibodies. PRNT revealed that sera from NHPs (African green monkeys and baboons) exhibited neutralizing activity against ZIKV (34.4%; 33/96), whereas a PRNT for yellow fever virus using NHP sera showed no neutralization activity. ZIKV genomic RNA was not detected in splenic tissues from NHPs, suggesting that the presence of anti-ZIKV neutralizing antibodies represented resolved infections. Our evidence suggests that ZIKV is maintained in NHP reservoirs in Zambia.

AB - Zika virus (ZIKV) circulation occurs between non-human primates (NHPs) in a sylvatic transmission cycle. To investigate evidence of flavivirus infection in NHPs in Zambia, we performed a plaque reduction neutralization test (PRNT) to quantify neutralizing antibodies. PRNT revealed that sera from NHPs (African green monkeys and baboons) exhibited neutralizing activity against ZIKV (34.4%; 33/96), whereas a PRNT for yellow fever virus using NHP sera showed no neutralization activity. ZIKV genomic RNA was not detected in splenic tissues from NHPs, suggesting that the presence of anti-ZIKV neutralizing antibodies represented resolved infections. Our evidence suggests that ZIKV is maintained in NHP reservoirs in Zambia.

U2 - 10.1007/s00705-019-04302-0

DO - 10.1007/s00705-019-04302-0

M3 - Article

SN - 0304-8608

VL - 164

SP - 2165

EP - 2170

JO - Archives of Virology

JF - Archives of Virology

IS - 8

ER -

Serological evidence of Zika virus infection in non-human primates in Zambia

Abstract

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