Sequence of the cDNA coding for the lethal neurotoxin Tx1 from the Brazilian "armed" spider Phoneutria nigriventer predicts the synthesis and processing of a preprotoxin

Marcelo R.V. Diniz; Mark J.I. Paine; Carlos R. Diniz; R. David G. Theakston; Julian M. Crampton

doi:10.1016/s0021-9258(18)82260-8

Sequence of the cDNA coding for the lethal neurotoxin Tx1 from the Brazilian "armed" spider Phoneutria nigriventer predicts the synthesis and processing of a preprotoxin

Marcelo R.V. Diniz
, Mark J.I. Paine
, Carlos R. Diniz
, R. David G. Theakston
, Julian M. Crampton

Vector Biology

Fundação Ezequiel Dias
Liverpool School of Tropical Medicine
Snake Venom Research Unit
Wolfson Unit of Molecular Genetics

Research output: Contribution to journal › Article › peer-review

45 Citations (Scopus)

Abstract

A cDNA library was constructed from the venom glands of the Brazilian "armed" spider, Phoneutria nigriventer, and a clone coding for Tx1, a lethal toxin, was identified and sequenced. The sequence data derived from this cDNA clone combined with the previously determined amino acid sequence predict that Tx1 is initially synthesized as a preprotoxin. Four segments (comprising the signal sequence, a short, 15-amino acid, glutamate-rich sequence, the functional toxin, and 2 glycine residues) can be distinguished. The structure of the preprotoxin and the proposed processing steps required to form the mature Tx1 toxin show similarities with the synthesis and processing of ω-agatoxin IA.

Original language	English
Pages (from-to)	15340-15342
Number of pages	3
Journal	Journal of Biological Chemistry
Volume	268
Issue number	21
DOIs	https://doi.org/10.1016/s0021-9258(18)82260-8
Publication status	Published - 25 Jul 1993

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10.1016/s0021-9258(18)82260-8

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title = "Sequence of the cDNA coding for the lethal neurotoxin Tx1 from the Brazilian {"}armed{"} spider Phoneutria nigriventer predicts the synthesis and processing of a preprotoxin",

abstract = "A cDNA library was constructed from the venom glands of the Brazilian {"}armed{"} spider, Phoneutria nigriventer, and a clone coding for Tx1, a lethal toxin, was identified and sequenced. The sequence data derived from this cDNA clone combined with the previously determined amino acid sequence predict that Tx1 is initially synthesized as a preprotoxin. Four segments (comprising the signal sequence, a short, 15-amino acid, glutamate-rich sequence, the functional toxin, and 2 glycine residues) can be distinguished. The structure of the preprotoxin and the proposed processing steps required to form the mature Tx1 toxin show similarities with the synthesis and processing of ω-agatoxin IA.",

author = "Diniz, \{Marcelo R.V.\} and Paine, \{Mark J.I.\} and Diniz, \{Carlos R.\} and Theakston, \{R. David G.\} and Crampton, \{Julian M.\}",

year = "1993",

month = jul,

day = "25",

doi = "10.1016/s0021-9258(18)82260-8",

language = "English",

volume = "268",

pages = "15340--15342",

journal = "Journal of Biological Chemistry",

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publisher = "American Society for Biochemistry and Molecular Biology Inc.",

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Sequence of the cDNA coding for the lethal neurotoxin Tx1 from the Brazilian "armed" spider Phoneutria nigriventer predicts the synthesis and processing of a preprotoxin. / Diniz, Marcelo R.V.; Paine, Mark J.I.; Diniz, Carlos R. et al.
In: Journal of Biological Chemistry, Vol. 268, No. 21, 25.07.1993, p. 15340-15342.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Sequence of the cDNA coding for the lethal neurotoxin Tx1 from the Brazilian "armed" spider Phoneutria nigriventer predicts the synthesis and processing of a preprotoxin

AU - Diniz, Marcelo R.V.

AU - Paine, Mark J.I.

AU - Diniz, Carlos R.

AU - Theakston, R. David G.

AU - Crampton, Julian M.

PY - 1993/7/25

Y1 - 1993/7/25

N2 - A cDNA library was constructed from the venom glands of the Brazilian "armed" spider, Phoneutria nigriventer, and a clone coding for Tx1, a lethal toxin, was identified and sequenced. The sequence data derived from this cDNA clone combined with the previously determined amino acid sequence predict that Tx1 is initially synthesized as a preprotoxin. Four segments (comprising the signal sequence, a short, 15-amino acid, glutamate-rich sequence, the functional toxin, and 2 glycine residues) can be distinguished. The structure of the preprotoxin and the proposed processing steps required to form the mature Tx1 toxin show similarities with the synthesis and processing of ω-agatoxin IA.

AB - A cDNA library was constructed from the venom glands of the Brazilian "armed" spider, Phoneutria nigriventer, and a clone coding for Tx1, a lethal toxin, was identified and sequenced. The sequence data derived from this cDNA clone combined with the previously determined amino acid sequence predict that Tx1 is initially synthesized as a preprotoxin. Four segments (comprising the signal sequence, a short, 15-amino acid, glutamate-rich sequence, the functional toxin, and 2 glycine residues) can be distinguished. The structure of the preprotoxin and the proposed processing steps required to form the mature Tx1 toxin show similarities with the synthesis and processing of ω-agatoxin IA.

U2 - 10.1016/s0021-9258(18)82260-8

DO - 10.1016/s0021-9258(18)82260-8

M3 - Article

C2 - 8340362

AN - SCOPUS:0027234680

SN - 0021-9258

VL - 268

SP - 15340

EP - 15342

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 21

ER -

Sequence of the cDNA coding for the lethal neurotoxin Tx1 from the Brazilian "armed" spider Phoneutria nigriventer predicts the synthesis and processing of a preprotoxin

Abstract

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