TY - JOUR
T1 - Sequence of the cDNA coding for the lethal neurotoxin Tx1 from the Brazilian "armed" spider Phoneutria nigriventer predicts the synthesis and processing of a preprotoxin
AU - Diniz, Marcelo R.V.
AU - Paine, Mark J.I.
AU - Diniz, Carlos R.
AU - Theakston, R. David G.
AU - Crampton, Julian M.
PY - 1993/7/25
Y1 - 1993/7/25
N2 - A cDNA library was constructed from the venom glands of the Brazilian "armed" spider, Phoneutria nigriventer, and a clone coding for Tx1, a lethal toxin, was identified and sequenced. The sequence data derived from this cDNA clone combined with the previously determined amino acid sequence predict that Tx1 is initially synthesized as a preprotoxin. Four segments (comprising the signal sequence, a short, 15-amino acid, glutamate-rich sequence, the functional toxin, and 2 glycine residues) can be distinguished. The structure of the preprotoxin and the proposed processing steps required to form the mature Tx1 toxin show similarities with the synthesis and processing of ω-agatoxin IA.
AB - A cDNA library was constructed from the venom glands of the Brazilian "armed" spider, Phoneutria nigriventer, and a clone coding for Tx1, a lethal toxin, was identified and sequenced. The sequence data derived from this cDNA clone combined with the previously determined amino acid sequence predict that Tx1 is initially synthesized as a preprotoxin. Four segments (comprising the signal sequence, a short, 15-amino acid, glutamate-rich sequence, the functional toxin, and 2 glycine residues) can be distinguished. The structure of the preprotoxin and the proposed processing steps required to form the mature Tx1 toxin show similarities with the synthesis and processing of ω-agatoxin IA.
U2 - 10.1016/s0021-9258(18)82260-8
DO - 10.1016/s0021-9258(18)82260-8
M3 - Article
C2 - 8340362
AN - SCOPUS:0027234680
SN - 0021-9258
VL - 268
SP - 15340
EP - 15342
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 21
ER -