Semen-Derived Amyloid Fibrils Drastically Enhance HIV Infection

Jan Münch; Elke Rücker; Ludger Ständker; Knut Adermann; Christine Goffinet; Michael Schindler; Steffen Wildum; Raghavan Chinnadurai; Devi Rajan; Anke Specht; Guillermo Giménez-Gallego; Pedro Cuevas Sánchez; Douglas M. Fowler; Atanas Koulov; Jeffery W. Kelly; Walther Mothes; Jean Charles Grivel; Leonid Margolis; Oliver T. Keppler; Wolf Georg Forssmann; Frank Kirchhoff

doi:10.1016/j.cell.2007.10.014

Semen-Derived Amyloid Fibrils Drastically Enhance HIV Infection

Jan Münch, Elke Rücker, Ludger Ständker, Knut Adermann, Christine Goffinet, Michael Schindler, Steffen Wildum, Raghavan Chinnadurai, Devi Rajan, Anke Specht, Guillermo Giménez-Gallego, Pedro Cuevas Sánchez, Douglas M. Fowler, Atanas Koulov, Jeffery W. Kelly, Walther Mothes, Jean Charles Grivel, Leonid Margolis, Oliver T. Keppler, Wolf Georg ForssmannFrank Kirchhoff

Research output: Contribution to journal › Article › peer-review

479 Citations (Scopus)

Abstract

Sexual intercourse is the major route of HIV transmission. To identify endogenous factors that affect the efficiency of sexual viral transmission, we screened a complex peptide/protein library derived from human semen. We show that naturally occurring fragments of the abundant semen marker prostatic acidic phosphatase (PAP) form amyloid fibrils. These fibrils, termed Semen-derived Enhancer of Virus Infection (SEVI), capture HIV virions and promote their attachment to target cells, thereby enhancing the infectious virus titer by several orders of magnitude. Physiological concentrations of SEVI amplified HIV infection of T cells, macrophages, ex vivo human tonsillar tissues, and transgenic rats in vivo, as well as trans-HIV infection of T cells by dendritic or epithelial cells. Amyloidogenic PAP fragments are abundant in seminal fluid and boost semen-mediated enhancement of HIV infection. Thus, they may play an important role in sexual transmission of HIV and could represent new targets for its prevention.

Original language	English
Pages (from-to)	1059-1071
Number of pages	13
Journal	Cell
Volume	131
Issue number	6
DOIs	https://doi.org/10.1016/j.cell.2007.10.014
Publication status	Published - 14 Dec 2007
Externally published	Yes

Keywords

HUMDISEASE
PROTEINS

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.cell.2007.10.014

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Münch, J., Rücker, E., Ständker, L., Adermann, K., Goffinet, C., Schindler, M., Wildum, S., Chinnadurai, R., Rajan, D., Specht, A., Giménez-Gallego, G., Sánchez, P. C., Fowler, D. M., Koulov, A., Kelly, J. W., Mothes, W., Grivel, J. C., Margolis, L., Keppler, O. T., ... Kirchhoff, F. (2007). Semen-Derived Amyloid Fibrils Drastically Enhance HIV Infection. Cell, 131(6), 1059-1071. https://doi.org/10.1016/j.cell.2007.10.014

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title = "Semen-Derived Amyloid Fibrils Drastically Enhance HIV Infection",

abstract = "Sexual intercourse is the major route of HIV transmission. To identify endogenous factors that affect the efficiency of sexual viral transmission, we screened a complex peptide/protein library derived from human semen. We show that naturally occurring fragments of the abundant semen marker prostatic acidic phosphatase (PAP) form amyloid fibrils. These fibrils, termed Semen-derived Enhancer of Virus Infection (SEVI), capture HIV virions and promote their attachment to target cells, thereby enhancing the infectious virus titer by several orders of magnitude. Physiological concentrations of SEVI amplified HIV infection of T cells, macrophages, ex vivo human tonsillar tissues, and transgenic rats in vivo, as well as trans-HIV infection of T cells by dendritic or epithelial cells. Amyloidogenic PAP fragments are abundant in seminal fluid and boost semen-mediated enhancement of HIV infection. Thus, they may play an important role in sexual transmission of HIV and could represent new targets for its prevention.",

keywords = "HUMDISEASE, PROTEINS",

author = "Jan M{\"u}nch and Elke R{\"u}cker and Ludger St{\"a}ndker and Knut Adermann and Christine Goffinet and Michael Schindler and Steffen Wildum and Raghavan Chinnadurai and Devi Rajan and Anke Specht and Guillermo Gim{\'e}nez-Gallego and S{\'a}nchez, \{Pedro Cuevas\} and Fowler, \{Douglas M.\} and Atanas Koulov and Kelly, \{Jeffery W.\} and Walther Mothes and Grivel, \{Jean Charles\} and Leonid Margolis and Keppler, \{Oliver T.\} and Forssmann, \{Wolf Georg\} and Frank Kirchhoff",

year = "2007",

month = dec,

day = "14",

doi = "10.1016/j.cell.2007.10.014",

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Münch, J, Rücker, E, Ständker, L, Adermann, K, Goffinet, C, Schindler, M, Wildum, S, Chinnadurai, R, Rajan, D, Specht, A, Giménez-Gallego, G, Sánchez, PC, Fowler, DM, Koulov, A, Kelly, JW, Mothes, W, Grivel, JC, Margolis, L, Keppler, OT, Forssmann, WG & Kirchhoff, F 2007, 'Semen-Derived Amyloid Fibrils Drastically Enhance HIV Infection', Cell, vol. 131, no. 6, pp. 1059-1071. https://doi.org/10.1016/j.cell.2007.10.014

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AU - Münch, Jan

AU - Rücker, Elke

AU - Ständker, Ludger

AU - Adermann, Knut

AU - Goffinet, Christine

AU - Schindler, Michael

AU - Wildum, Steffen

AU - Chinnadurai, Raghavan

AU - Rajan, Devi

AU - Specht, Anke

AU - Giménez-Gallego, Guillermo

AU - Sánchez, Pedro Cuevas

AU - Fowler, Douglas M.

AU - Koulov, Atanas

AU - Kelly, Jeffery W.

AU - Mothes, Walther

AU - Grivel, Jean Charles

AU - Margolis, Leonid

AU - Keppler, Oliver T.

AU - Forssmann, Wolf Georg

AU - Kirchhoff, Frank

PY - 2007/12/14

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N2 - Sexual intercourse is the major route of HIV transmission. To identify endogenous factors that affect the efficiency of sexual viral transmission, we screened a complex peptide/protein library derived from human semen. We show that naturally occurring fragments of the abundant semen marker prostatic acidic phosphatase (PAP) form amyloid fibrils. These fibrils, termed Semen-derived Enhancer of Virus Infection (SEVI), capture HIV virions and promote their attachment to target cells, thereby enhancing the infectious virus titer by several orders of magnitude. Physiological concentrations of SEVI amplified HIV infection of T cells, macrophages, ex vivo human tonsillar tissues, and transgenic rats in vivo, as well as trans-HIV infection of T cells by dendritic or epithelial cells. Amyloidogenic PAP fragments are abundant in seminal fluid and boost semen-mediated enhancement of HIV infection. Thus, they may play an important role in sexual transmission of HIV and could represent new targets for its prevention.

AB - Sexual intercourse is the major route of HIV transmission. To identify endogenous factors that affect the efficiency of sexual viral transmission, we screened a complex peptide/protein library derived from human semen. We show that naturally occurring fragments of the abundant semen marker prostatic acidic phosphatase (PAP) form amyloid fibrils. These fibrils, termed Semen-derived Enhancer of Virus Infection (SEVI), capture HIV virions and promote their attachment to target cells, thereby enhancing the infectious virus titer by several orders of magnitude. Physiological concentrations of SEVI amplified HIV infection of T cells, macrophages, ex vivo human tonsillar tissues, and transgenic rats in vivo, as well as trans-HIV infection of T cells by dendritic or epithelial cells. Amyloidogenic PAP fragments are abundant in seminal fluid and boost semen-mediated enhancement of HIV infection. Thus, they may play an important role in sexual transmission of HIV and could represent new targets for its prevention.

KW - HUMDISEASE

KW - PROTEINS

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DO - 10.1016/j.cell.2007.10.014

M3 - Article

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VL - 131

SP - 1059

EP - 1071

JO - Cell

JF - Cell

IS - 6

ER -

Semen-Derived Amyloid Fibrils Drastically Enhance HIV Infection

Abstract

Keywords

UN SDGs

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