Selective determination, in plasma, of artemether and its major metabolite, dihydroartemisinin, by high-performance liquid chromatography with ultraviolet detection

C. G. Thomas; Steve Ward; G. Edwards

doi:10.1016/0378-4347(92)80355-t

Selective determination, in plasma, of artemether and its major metabolite, dihydroartemisinin, by high-performance liquid chromatography with ultraviolet detection

C. G. Thomas
, Steve Ward
, G. Edwards

Liverpool School of Tropical Medicine

Research output: Contribution to journal › Article › peer-review

68 Citations (Scopus)

Abstract

A sensitive and selective reversed-phase high-performance liquid chromatographic method for the determination of artemether and its major metabolite dihydroartemisinin in plasma has been developed. It involves extraction of plasma with dichloromethane, solid-phase separation of the two analytes and acid decomposition prior to chromatography on a C18 Spherisorb column with a mobile phase of acetonitrile-water (50:50, v/v). Run time is 30 min. The assay satisfies all of the criteria required for use in clinical pharmacokinetic studies.

Original language	English
Pages (from-to)	131-136
Number of pages	6
Journal	Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
Volume	583
Issue number	1
DOIs	https://doi.org/10.1016/0378-4347(92)80355-t
Publication status	Published - 27 Nov 1992

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title = "Selective determination, in plasma, of artemether and its major metabolite, dihydroartemisinin, by high-performance liquid chromatography with ultraviolet detection",

abstract = "A sensitive and selective reversed-phase high-performance liquid chromatographic method for the determination of artemether and its major metabolite dihydroartemisinin in plasma has been developed. It involves extraction of plasma with dichloromethane, solid-phase separation of the two analytes and acid decomposition prior to chromatography on a C18 Spherisorb column with a mobile phase of acetonitrile-water (50:50, v/v). Run time is 30 min. The assay satisfies all of the criteria required for use in clinical pharmacokinetic studies.",

author = "Thomas, \{C. G.\} and Steve Ward and G. Edwards",

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language = "English",

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Thomas, CG, Ward, S & Edwards, G 1992, 'Selective determination, in plasma, of artemether and its major metabolite, dihydroartemisinin, by high-performance liquid chromatography with ultraviolet detection', Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, vol. 583, no. 1, pp. 131-136. https://doi.org/10.1016/0378-4347(92)80355-t

Selective determination, in plasma, of artemether and its major metabolite, dihydroartemisinin, by high-performance liquid chromatography with ultraviolet detection. / Thomas, C. G.; Ward, Steve; Edwards, G.
In: Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, Vol. 583, No. 1, 27.11.1992, p. 131-136.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Selective determination, in plasma, of artemether and its major metabolite, dihydroartemisinin, by high-performance liquid chromatography with ultraviolet detection

AU - Thomas, C. G.

AU - Ward, Steve

AU - Edwards, G.

PY - 1992/11/27

Y1 - 1992/11/27

N2 - A sensitive and selective reversed-phase high-performance liquid chromatographic method for the determination of artemether and its major metabolite dihydroartemisinin in plasma has been developed. It involves extraction of plasma with dichloromethane, solid-phase separation of the two analytes and acid decomposition prior to chromatography on a C18 Spherisorb column with a mobile phase of acetonitrile-water (50:50, v/v). Run time is 30 min. The assay satisfies all of the criteria required for use in clinical pharmacokinetic studies.

AB - A sensitive and selective reversed-phase high-performance liquid chromatographic method for the determination of artemether and its major metabolite dihydroartemisinin in plasma has been developed. It involves extraction of plasma with dichloromethane, solid-phase separation of the two analytes and acid decomposition prior to chromatography on a C18 Spherisorb column with a mobile phase of acetonitrile-water (50:50, v/v). Run time is 30 min. The assay satisfies all of the criteria required for use in clinical pharmacokinetic studies.

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DO - 10.1016/0378-4347(92)80355-t

M3 - Article

VL - 583

SP - 131

EP - 136

JO - Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences

IS - 1

ER -

Selective determination, in plasma, of artemether and its major metabolite, dihydroartemisinin, by high-performance liquid chromatography with ultraviolet detection

Abstract

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