TY - JOUR
T1 - Selective determination, in plasma, of artemether and its major metabolite, dihydroartemisinin, by high-performance liquid chromatography with ultraviolet detection
AU - Thomas, C. G.
AU - Ward, Steve
AU - Edwards, G.
PY - 1992/11/27
Y1 - 1992/11/27
N2 - A sensitive and selective reversed-phase high-performance liquid chromatographic method for the determination of artemether and its major metabolite dihydroartemisinin in plasma has been developed. It involves extraction of plasma with dichloromethane, solid-phase separation of the two analytes and acid decomposition prior to chromatography on a C18 Spherisorb column with a mobile phase of acetonitrile-water (50:50, v/v). Run time is 30 min. The assay satisfies all of the criteria required for use in clinical pharmacokinetic studies.
AB - A sensitive and selective reversed-phase high-performance liquid chromatographic method for the determination of artemether and its major metabolite dihydroartemisinin in plasma has been developed. It involves extraction of plasma with dichloromethane, solid-phase separation of the two analytes and acid decomposition prior to chromatography on a C18 Spherisorb column with a mobile phase of acetonitrile-water (50:50, v/v). Run time is 30 min. The assay satisfies all of the criteria required for use in clinical pharmacokinetic studies.
U2 - 10.1016/0378-4347(92)80355-t
DO - 10.1016/0378-4347(92)80355-t
M3 - Article
VL - 583
SP - 131
EP - 136
JO - Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
IS - 1
ER -