Abstract
A sensitive and selective reversed-phase high-performance liquid chromatographic method for the determination of albendazole and its active metabolite albendazole sulphoxide in plasma has been developed. It involves single-step extraction of plasma with dichloromethane, evaporation of the solvent and chromatography on a μBondapak phenyl column with a mobile phase of water containing 1% (v/v) triethylamine-methanol-acetonitrile (70:10:20, v/v) at pH 3.1. Run time is 12 min. The assay satisfies all of the criteria required for use in clinical pharmacokinetic studies and possesses important advantages, notably speed and expense, over current methods.
| Original language | English |
|---|---|
| Pages (from-to) | 244-249 |
| Number of pages | 6 |
| Journal | Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences |
| Volume | 566 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 3 May 1991 |