PWE-130 The human gut mucosal cognate cellular response to live oral typhoid vaccination

Shaun Pennington; Ameeka Thompson; Adam Wright; Daniela Ferreira; Angela Wright; Brian Faragher; Stephen Gordon; M. A. Gordon

doi:10.1136/gutjnl-2012-302514d.130

PWE-130 The human gut mucosal cognate cellular response to live oral typhoid vaccination

Shaun Pennington, Ameeka Thompson, Adam Wright, Daniela Ferreira, Angela Wright, Brian Faragher, Stephen Gordon, M. A. Gordon

Liverpool School of Tropical Medicine

Research output: Contribution to conference › Paper

Abstract

Introduction: The human gut mucosal cellular response to oral vaccination has never been directly assessed. We studied the cognate cellular immune response to the live oral typhoid Ty21a vaccine in the gut mucosa of human volunteers, and compared it with that seen in peripheral blood.

Methods: 27 healthy volunteers were randomly assigned to a vaccinated (n=14) or a control (n=13) group for Ty21a typhoid vaccine. Peripheral blood was collected from all volunteers prior to vaccination and 18 days following immunisation or recruitment. Mucosal samples (15 jumbo biopsies from duodenum (n=25) ± colon (n=18)) were collected from all volunteers at gastroscopy +/− sigmoidoscopy on day 18. Mononuclear cells were isolated from mucosal tissue by disruption and collagenase digestion, and from blood by differential centrifugation. Cells were stimulated with Ty21a or control antigens, and stained for surface phenotype and intracellular cytokine production. Antigen-specific IFN-γ, TNF-α, and IL-2 production was determined

Original language	English
Pages	A349-A350
DOIs	https://doi.org/10.1136/gutjnl-2012-302514d.130
Publication status	Published - 1 Jul 2012

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title = "PWE-130 The human gut mucosal cognate cellular response to live oral typhoid vaccination",

abstract = "Introduction: The human gut mucosal cellular response to oral vaccination has never been directly assessed. We studied the cognate cellular immune response to the live oral typhoid Ty21a vaccine in the gut mucosa of human volunteers, and compared it with that seen in peripheral blood.Methods: 27 healthy volunteers were randomly assigned to a vaccinated (n=14) or a control (n=13) group for Ty21a typhoid vaccine. Peripheral blood was collected from all volunteers prior to vaccination and 18 days following immunisation or recruitment. Mucosal samples (15 jumbo biopsies from duodenum (n=25) ± colon (n=18)) were collected from all volunteers at gastroscopy +/− sigmoidoscopy on day 18. Mononuclear cells were isolated from mucosal tissue by disruption and collagenase digestion, and from blood by differential centrifugation. Cells were stimulated with Ty21a or control antigens, and stained for surface phenotype and intracellular cytokine production. Antigen-specific IFN-γ, TNF-α, and IL-2 production was determined",

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T1 - PWE-130 The human gut mucosal cognate cellular response to live oral typhoid vaccination

AU - Pennington, Shaun

AU - Thompson, Ameeka

AU - Wright, Adam

AU - Ferreira, Daniela

AU - Wright, Angela

AU - Faragher, Brian

AU - Gordon, Stephen

AU - Gordon, M. A.

PY - 2012/7/1

Y1 - 2012/7/1

N2 - Introduction: The human gut mucosal cellular response to oral vaccination has never been directly assessed. We studied the cognate cellular immune response to the live oral typhoid Ty21a vaccine in the gut mucosa of human volunteers, and compared it with that seen in peripheral blood.Methods: 27 healthy volunteers were randomly assigned to a vaccinated (n=14) or a control (n=13) group for Ty21a typhoid vaccine. Peripheral blood was collected from all volunteers prior to vaccination and 18 days following immunisation or recruitment. Mucosal samples (15 jumbo biopsies from duodenum (n=25) ± colon (n=18)) were collected from all volunteers at gastroscopy +/− sigmoidoscopy on day 18. Mononuclear cells were isolated from mucosal tissue by disruption and collagenase digestion, and from blood by differential centrifugation. Cells were stimulated with Ty21a or control antigens, and stained for surface phenotype and intracellular cytokine production. Antigen-specific IFN-γ, TNF-α, and IL-2 production was determined

AB - Introduction: The human gut mucosal cellular response to oral vaccination has never been directly assessed. We studied the cognate cellular immune response to the live oral typhoid Ty21a vaccine in the gut mucosa of human volunteers, and compared it with that seen in peripheral blood.Methods: 27 healthy volunteers were randomly assigned to a vaccinated (n=14) or a control (n=13) group for Ty21a typhoid vaccine. Peripheral blood was collected from all volunteers prior to vaccination and 18 days following immunisation or recruitment. Mucosal samples (15 jumbo biopsies from duodenum (n=25) ± colon (n=18)) were collected from all volunteers at gastroscopy +/− sigmoidoscopy on day 18. Mononuclear cells were isolated from mucosal tissue by disruption and collagenase digestion, and from blood by differential centrifugation. Cells were stimulated with Ty21a or control antigens, and stained for surface phenotype and intracellular cytokine production. Antigen-specific IFN-γ, TNF-α, and IL-2 production was determined

U2 - 10.1136/gutjnl-2012-302514d.130

DO - 10.1136/gutjnl-2012-302514d.130

M3 - Paper

SP - A349-A350

ER -

PWE-130 The human gut mucosal cognate cellular response to live oral typhoid vaccination

Abstract

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