Putative pleiotropic effects of the knockdown resistance (L1014F) allele on the life-history traits of Anopheles gambiae.

Adandé A Medjigbodo; Luc Djogbenou; Oswald Y Djihinto; Romaric B Akoton; Emmanuella Abbey; Rosaria M Kakossou; Eric G Sonounameto; Esther B J Salavi; Laurette Djossou; Athanase Badolo

doi:10.1186/s12936-021-04005-5

Putative pleiotropic effects of the knockdown resistance (L1014F) allele on the life-history traits of Anopheles gambiae.

Adandé A Medjigbodo, Luc Djogbenou, Oswald Y Djihinto, Romaric B Akoton, Emmanuella Abbey, Rosaria M Kakossou, Eric G Sonounameto, Esther B J Salavi, Laurette Djossou, Athanase Badolo

Vector Biology

Liverpool School of Tropical Medicine

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Background

Existing mechanisms of insecticide resistance are known to help the survival of mosquitoes following contact with chemical compounds, even though they could negatively affect the life-history traits of resistant malaria vectors. In West Africa, the knockdown resistance mechanism kdrR (L1014F) is the most common. However, little knowledge is available on its effects on mosquito life-history traits. The fitness effects associated with this knockdown resistance allele in Anopheles gambiae sensu stricto (s.s.) were investigated in an insecticide-free laboratory environment.

Methods

The life-history traits of Kisumu (susceptible) and KisKdr (kdr resistant) strains of An. gambiae s.s. were compared. Larval survivorship and pupation rate were assessed as well as fecundity and fertility of adult females. Female mosquitoes of both strains were directly blood fed through artificial membrane assays and then the blood-feeding success, blood volume and adult survivorship post-blood meal were assessed.

Results

The An. gambiae mosquitoes carrying the kdrR allele (KisKdr) laid a reduced number of eggs. The mean number of larvae in the susceptible strain Kisumu was three-fold overall higher than that seen in the KisKdr strain with a significant difference in hatching rates (81.89% in Kisumu vs 72.89% in KisKdr). The KisKdr larvae had a significant higher survivorship than that of Kisumu. The blood-feeding success was significantly higher in the resistant mosquitoes (84%) compared to the susceptible ones (34.75%). However, the mean blood volume was 1.36 µL/mg, 1.45 µL/mg and 1.68 µL/mg in Kisumu, homozygote and heterozygote KisKdr mosquitoes, respectively. After blood-feeding, the heterozygote KisKdr mosquitoes displayed highest survivorship when compared to that of Kisumu.

Conclusions

The presence of the knockdown resistance allele appears to impact the life-history traits, such as fecundity, fertility, larval survivorship, and blood-feeding behaviour in An. gambiae. These data could help to guide the implementation of more reliable strategies for the control of malaria vectors.

Original language	English
Article number	480
Pages (from-to)	e480
Journal	Malaria Journal
Volume	20
Issue number	1
Early online date	20 Dec 2021
DOIs	https://doi.org/10.1186/s12936-021-04005-5
Publication status	E-pub ahead of print - 20 Dec 2021

Keywords

Anopheles gambiae
Fitness effects
Kdr allele
Life-history traits
Malaria

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1186/s12936-021-04005-5Licence: CC BY

PMC8686585Final published version, 1.73 MBLicence: CC BY

Cite this

Medjigbodo, A. A., Djogbenou, L., Djihinto, O. Y., Akoton, R. B., Abbey, E., Kakossou, R. M., Sonounameto, E. G., Salavi, E. B. J., Djossou, L., & Badolo, A. (2021). Putative pleiotropic effects of the knockdown resistance (L1014F) allele on the life-history traits of Anopheles gambiae. Malaria Journal, 20(1), e480. Article 480. Advance online publication. https://doi.org/10.1186/s12936-021-04005-5

@article{126cd016cfa94015a4884c6ed3ccbc67,

title = "Putative pleiotropic effects of the knockdown resistance (L1014F) allele on the life-history traits of Anopheles gambiae.",

abstract = "BackgroundExisting mechanisms of insecticide resistance are known to help the survival of mosquitoes following contact with chemical compounds, even though they could negatively affect the life-history traits of resistant malaria vectors. In West Africa, the knockdown resistance mechanism kdrR (L1014F) is the most common. However, little knowledge is available on its effects on mosquito life-history traits. The fitness effects associated with this knockdown resistance allele in Anopheles gambiae sensu stricto (s.s.) were investigated in an insecticide-free laboratory environment.MethodsThe life-history traits of Kisumu (susceptible) and KisKdr (kdr resistant) strains of An. gambiae s.s. were compared. Larval survivorship and pupation rate were assessed as well as fecundity and fertility of adult females. Female mosquitoes of both strains were directly blood fed through artificial membrane assays and then the blood-feeding success, blood volume and adult survivorship post-blood meal were assessed.ResultsThe An. gambiae mosquitoes carrying the kdrR allele (KisKdr) laid a reduced number of eggs. The mean number of larvae in the susceptible strain Kisumu was three-fold overall higher than that seen in the KisKdr strain with a significant difference in hatching rates (81.89\% in Kisumu vs 72.89\% in KisKdr). The KisKdr larvae had a significant higher survivorship than that of Kisumu. The blood-feeding success was significantly higher in the resistant mosquitoes (84\%) compared to the susceptible ones (34.75\%). However, the mean blood volume was 1.36 µL/mg, 1.45 µL/mg and 1.68 µL/mg in Kisumu, homozygote and heterozygote KisKdr mosquitoes, respectively. After blood-feeding, the heterozygote KisKdr mosquitoes displayed highest survivorship when compared to that of Kisumu.ConclusionsThe presence of the knockdown resistance allele appears to impact the life-history traits, such as fecundity, fertility, larval survivorship, and blood-feeding behaviour in An. gambiae. These data could help to guide the implementation of more reliable strategies for the control of malaria vectors.",

keywords = "Anopheles gambiae, Fitness effects, Kdr allele, Life-history traits, Malaria",

author = "Medjigbodo, \{Adand{\'e} A\} and Luc Djogbenou and Djihinto, \{Oswald Y\} and Akoton, \{Romaric B\} and Emmanuella Abbey and Kakossou, \{Rosaria M\} and Sonounameto, \{Eric G\} and Salavi, \{Esther B J\} and Laurette Djossou and Athanase Badolo",

year = "2021",

month = dec,

day = "20",

doi = "10.1186/s12936-021-04005-5",

language = "English",

volume = "20",

pages = "e480",

journal = "Malaria Journal",

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publisher = "BioMed Central Ltd",

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T1 - Putative pleiotropic effects of the knockdown resistance (L1014F) allele on the life-history traits of Anopheles gambiae.

AU - Medjigbodo, Adandé A

AU - Djogbenou, Luc

AU - Djihinto, Oswald Y

AU - Akoton, Romaric B

AU - Abbey, Emmanuella

AU - Kakossou, Rosaria M

AU - Sonounameto, Eric G

AU - Salavi, Esther B J

AU - Djossou, Laurette

AU - Badolo, Athanase

PY - 2021/12/20

Y1 - 2021/12/20

N2 - BackgroundExisting mechanisms of insecticide resistance are known to help the survival of mosquitoes following contact with chemical compounds, even though they could negatively affect the life-history traits of resistant malaria vectors. In West Africa, the knockdown resistance mechanism kdrR (L1014F) is the most common. However, little knowledge is available on its effects on mosquito life-history traits. The fitness effects associated with this knockdown resistance allele in Anopheles gambiae sensu stricto (s.s.) were investigated in an insecticide-free laboratory environment.MethodsThe life-history traits of Kisumu (susceptible) and KisKdr (kdr resistant) strains of An. gambiae s.s. were compared. Larval survivorship and pupation rate were assessed as well as fecundity and fertility of adult females. Female mosquitoes of both strains were directly blood fed through artificial membrane assays and then the blood-feeding success, blood volume and adult survivorship post-blood meal were assessed.ResultsThe An. gambiae mosquitoes carrying the kdrR allele (KisKdr) laid a reduced number of eggs. The mean number of larvae in the susceptible strain Kisumu was three-fold overall higher than that seen in the KisKdr strain with a significant difference in hatching rates (81.89% in Kisumu vs 72.89% in KisKdr). The KisKdr larvae had a significant higher survivorship than that of Kisumu. The blood-feeding success was significantly higher in the resistant mosquitoes (84%) compared to the susceptible ones (34.75%). However, the mean blood volume was 1.36 µL/mg, 1.45 µL/mg and 1.68 µL/mg in Kisumu, homozygote and heterozygote KisKdr mosquitoes, respectively. After blood-feeding, the heterozygote KisKdr mosquitoes displayed highest survivorship when compared to that of Kisumu.ConclusionsThe presence of the knockdown resistance allele appears to impact the life-history traits, such as fecundity, fertility, larval survivorship, and blood-feeding behaviour in An. gambiae. These data could help to guide the implementation of more reliable strategies for the control of malaria vectors.

AB - BackgroundExisting mechanisms of insecticide resistance are known to help the survival of mosquitoes following contact with chemical compounds, even though they could negatively affect the life-history traits of resistant malaria vectors. In West Africa, the knockdown resistance mechanism kdrR (L1014F) is the most common. However, little knowledge is available on its effects on mosquito life-history traits. The fitness effects associated with this knockdown resistance allele in Anopheles gambiae sensu stricto (s.s.) were investigated in an insecticide-free laboratory environment.MethodsThe life-history traits of Kisumu (susceptible) and KisKdr (kdr resistant) strains of An. gambiae s.s. were compared. Larval survivorship and pupation rate were assessed as well as fecundity and fertility of adult females. Female mosquitoes of both strains were directly blood fed through artificial membrane assays and then the blood-feeding success, blood volume and adult survivorship post-blood meal were assessed.ResultsThe An. gambiae mosquitoes carrying the kdrR allele (KisKdr) laid a reduced number of eggs. The mean number of larvae in the susceptible strain Kisumu was three-fold overall higher than that seen in the KisKdr strain with a significant difference in hatching rates (81.89% in Kisumu vs 72.89% in KisKdr). The KisKdr larvae had a significant higher survivorship than that of Kisumu. The blood-feeding success was significantly higher in the resistant mosquitoes (84%) compared to the susceptible ones (34.75%). However, the mean blood volume was 1.36 µL/mg, 1.45 µL/mg and 1.68 µL/mg in Kisumu, homozygote and heterozygote KisKdr mosquitoes, respectively. After blood-feeding, the heterozygote KisKdr mosquitoes displayed highest survivorship when compared to that of Kisumu.ConclusionsThe presence of the knockdown resistance allele appears to impact the life-history traits, such as fecundity, fertility, larval survivorship, and blood-feeding behaviour in An. gambiae. These data could help to guide the implementation of more reliable strategies for the control of malaria vectors.

KW - Anopheles gambiae

KW - Fitness effects

KW - Kdr allele

KW - Life-history traits

KW - Malaria

U2 - 10.1186/s12936-021-04005-5

DO - 10.1186/s12936-021-04005-5

M3 - Article

SN - 1475-2875

VL - 20

SP - e480

JO - Malaria Journal

JF - Malaria Journal

IS - 1

M1 - 480

ER -

Putative pleiotropic effects of the knockdown resistance (L1014F) allele on the life-history traits of Anopheles gambiae.

Abstract

Keywords

UN SDGs

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