Proportions of circulating follicular helper T cells are reduced and correlate with memory B cells in HIV-infected children

Daniel M. Muema; Gladys N. Macharia; Babatunde A. Olusola; Amin S. Hassan; Greg W. Fegan; James A. Berkley; Britta Urban; Eunice W. Nduati

doi:10.1371/journal.pone.0175570

Proportions of circulating follicular helper T cells are reduced and correlate with memory B cells in HIV-infected children

Daniel M. Muema, Gladys N. Macharia, Babatunde A. Olusola, Amin S. Hassan, Greg W. Fegan, James A. Berkley, Britta Urban, Eunice W. Nduati

Tropical Disease Biology

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Introduction

HIV causes defects in memory B cells in children, but the mechanisms of those defects have not been fully elucidated. One possible mechanism is the lack of T-cell help to B cells during immune reactions. However, few studies have assessed the effect of HIV on follicular helper T cells (TFH cells) in children.

Methods

In this study, follicular-homing CD4 T cells and memory B cells were assessed in HIV-infected children and compared with children from the community. CXCR5 and CD45RO were used as markers of follicular-homing T cells and memory T cells, respectively. Memory TFH cells were identified as CD3+CD8-CD4+CXCR5+CD45RO+PD1+. Central memory T cells were identified based on CCR7 expression. Relationship between the proportions of follicular-homing CD4 T cells and memory B cells were determined in multivariable regression models.

Results

Highly viremic HIV-infected children had lower proportions of memory TFH cells when compared with community control children. In multivariable analyses, high proportions of memory TFH cells were associated with increased percentages of resting memory B cells after adjusting for other covariates.

Conclusion

The impact of HIV on follicular helper T cells could influence the accumulation of memory B cells in HIV-infected children.

Original language	English
Article number	e0175570
Pages (from-to)	e0175570
Journal	PLoS ONE
Volume	12
Issue number	4
DOIs	https://doi.org/10.1371/journal.pone.0175570
Publication status	Published - 26 Apr 2017

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Plos ONE 12 4 e0175570Final published version, 929 KBLicence: CC BY

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@article{c4aaac62ccea45959cbee17a39310f5a,

title = "Proportions of circulating follicular helper T cells are reduced and correlate with memory B cells in HIV-infected children",

abstract = "Introduction HIV causes defects in memory B cells in children, but the mechanisms of those defects have not been fully elucidated. One possible mechanism is the lack of T-cell help to B cells during immune reactions. However, few studies have assessed the effect of HIV on follicular helper T cells (TFH cells) in children. Methods In this study, follicular-homing CD4 T cells and memory B cells were assessed in HIV-infected children and compared with children from the community. CXCR5 and CD45RO were used as markers of follicular-homing T cells and memory T cells, respectively. Memory TFH cells were identified as CD3+CD8-CD4+CXCR5+CD45RO+PD1+. Central memory T cells were identified based on CCR7 expression. Relationship between the proportions of follicular-homing CD4 T cells and memory B cells were determined in multivariable regression models. Results Highly viremic HIV-infected children had lower proportions of memory TFH cells when compared with community control children. In multivariable analyses, high proportions of memory TFH cells were associated with increased percentages of resting memory B cells after adjusting for other covariates. Conclusion The impact of HIV on follicular helper T cells could influence the accumulation of memory B cells in HIV-infected children.",

author = "Muema, \{Daniel M.\} and Macharia, \{Gladys N.\} and Olusola, \{Babatunde A.\} and Hassan, \{Amin S.\} and Fegan, \{Greg W.\} and Berkley, \{James A.\} and Britta Urban and Nduati, \{Eunice W.\}",

year = "2017",

month = apr,

day = "26",

doi = "10.1371/journal.pone.0175570",

language = "English",

volume = "12",

pages = "e0175570",

journal = "PLoS ONE",

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TY - JOUR

T1 - Proportions of circulating follicular helper T cells are reduced and correlate with memory B cells in HIV-infected children

AU - Muema, Daniel M.

AU - Macharia, Gladys N.

AU - Olusola, Babatunde A.

AU - Hassan, Amin S.

AU - Fegan, Greg W.

AU - Berkley, James A.

AU - Urban, Britta

AU - Nduati, Eunice W.

PY - 2017/4/26

Y1 - 2017/4/26

N2 - Introduction HIV causes defects in memory B cells in children, but the mechanisms of those defects have not been fully elucidated. One possible mechanism is the lack of T-cell help to B cells during immune reactions. However, few studies have assessed the effect of HIV on follicular helper T cells (TFH cells) in children. Methods In this study, follicular-homing CD4 T cells and memory B cells were assessed in HIV-infected children and compared with children from the community. CXCR5 and CD45RO were used as markers of follicular-homing T cells and memory T cells, respectively. Memory TFH cells were identified as CD3+CD8-CD4+CXCR5+CD45RO+PD1+. Central memory T cells were identified based on CCR7 expression. Relationship between the proportions of follicular-homing CD4 T cells and memory B cells were determined in multivariable regression models. Results Highly viremic HIV-infected children had lower proportions of memory TFH cells when compared with community control children. In multivariable analyses, high proportions of memory TFH cells were associated with increased percentages of resting memory B cells after adjusting for other covariates. Conclusion The impact of HIV on follicular helper T cells could influence the accumulation of memory B cells in HIV-infected children.

AB - Introduction HIV causes defects in memory B cells in children, but the mechanisms of those defects have not been fully elucidated. One possible mechanism is the lack of T-cell help to B cells during immune reactions. However, few studies have assessed the effect of HIV on follicular helper T cells (TFH cells) in children. Methods In this study, follicular-homing CD4 T cells and memory B cells were assessed in HIV-infected children and compared with children from the community. CXCR5 and CD45RO were used as markers of follicular-homing T cells and memory T cells, respectively. Memory TFH cells were identified as CD3+CD8-CD4+CXCR5+CD45RO+PD1+. Central memory T cells were identified based on CCR7 expression. Relationship between the proportions of follicular-homing CD4 T cells and memory B cells were determined in multivariable regression models. Results Highly viremic HIV-infected children had lower proportions of memory TFH cells when compared with community control children. In multivariable analyses, high proportions of memory TFH cells were associated with increased percentages of resting memory B cells after adjusting for other covariates. Conclusion The impact of HIV on follicular helper T cells could influence the accumulation of memory B cells in HIV-infected children.

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DO - 10.1371/journal.pone.0175570

M3 - Article

VL - 12

SP - e0175570

JO - PLoS ONE

JF - PLoS ONE

IS - 4

M1 - e0175570

ER -

Proportions of circulating follicular helper T cells are reduced and correlate with memory B cells in HIV-infected children

Abstract

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