Poor Diagnostic Accuracy of Commercial Antibody-Based Assays for the Diagnosis of Acute Chikungunya Infection

Stuart D. Blacksell; Ampai Tanganuchitcharnchai; Richard G. Jarman; Robert V. Gibbons; Daniel H. Paris; Mark S. Bailey; Nicholas P.J. Day; Ranjan Premaratna; David Lalloo; H. Janaka De Silva

doi:10.1128/cvi.05288-11

Poor Diagnostic Accuracy of Commercial Antibody-Based Assays for the Diagnosis of Acute Chikungunya Infection

Stuart D. Blacksell, Ampai Tanganuchitcharnchai, Richard G. Jarman, Robert V. Gibbons, Daniel H. Paris, Mark S. Bailey, Nicholas P.J. Day, Ranjan Premaratna, David Lalloo, H. Janaka De Silva

Clinical Sciences

Research output: Contribution to journal › Article › peer-review

42 Citations (Scopus)

Abstract

A Sri Lankan fever cohort (n = 292 patients; 17.8% prevalence) was used to assess two standard diagnostic Chikungunya IgM tests. The immunochromatographic test (ICT) acute sample sensitivity (SN) was 1.9 to 3.9%, and specificity (SP) was 92.5 to 95.0%. The enzyme-linked immunosorbent assay (ELISA) gave an acute sample SN of 3.9% and an SP of 92.5% and a convalescent sample SN of 84% and an SP of 91%. These assays are not suitable for the acute diagnosis of Chikungunya virus infection.

Original language	English
Pages (from-to)	1773-1775
Number of pages	3
Journal	Clinical and Vaccine Immunology
Volume	18
Issue number	10
DOIs	https://doi.org/10.1128/cvi.05288-11
Publication status	Published - 1 Oct 2011

Access to Document

10.1128/cvi.05288-11

Cite this

Blacksell, S. D., Tanganuchitcharnchai, A., Jarman, R. G., Gibbons, R. V., Paris, D. H., Bailey, M. S., Day, N. P. J., Premaratna, R., Lalloo, D., & De Silva, H. J. (2011). Poor Diagnostic Accuracy of Commercial Antibody-Based Assays for the Diagnosis of Acute Chikungunya Infection. Clinical and Vaccine Immunology, 18(10), 1773-1775. https://doi.org/10.1128/cvi.05288-11

@article{3423e549558940dba4201bfeb02d8f47,

title = "Poor Diagnostic Accuracy of Commercial Antibody-Based Assays for the Diagnosis of Acute Chikungunya Infection",

abstract = "A Sri Lankan fever cohort (n = 292 patients; 17.8\% prevalence) was used to assess two standard diagnostic Chikungunya IgM tests. The immunochromatographic test (ICT) acute sample sensitivity (SN) was 1.9 to 3.9\%, and specificity (SP) was 92.5 to 95.0\%. The enzyme-linked immunosorbent assay (ELISA) gave an acute sample SN of 3.9\% and an SP of 92.5\% and a convalescent sample SN of 84\% and an SP of 91\%. These assays are not suitable for the acute diagnosis of Chikungunya virus infection.",

author = "Blacksell, \{Stuart D.\} and Ampai Tanganuchitcharnchai and Jarman, \{Richard G.\} and Gibbons, \{Robert V.\} and Paris, \{Daniel H.\} and Bailey, \{Mark S.\} and Day, \{Nicholas P.J.\} and Ranjan Premaratna and David Lalloo and \{De Silva\}, \{H. Janaka\}",

year = "2011",

month = oct,

day = "1",

doi = "10.1128/cvi.05288-11",

language = "English",

volume = "18",

pages = "1773--1775",

journal = "Clinical and Vaccine Immunology",

issn = "1556-6811",

publisher = "American Society for Microbiology",

number = "10",

}

TY - JOUR

T1 - Poor Diagnostic Accuracy of Commercial Antibody-Based Assays for the Diagnosis of Acute Chikungunya Infection

AU - Blacksell, Stuart D.

AU - Tanganuchitcharnchai, Ampai

AU - Jarman, Richard G.

AU - Gibbons, Robert V.

AU - Paris, Daniel H.

AU - Bailey, Mark S.

AU - Day, Nicholas P.J.

AU - Premaratna, Ranjan

AU - Lalloo, David

AU - De Silva, H. Janaka

PY - 2011/10/1

Y1 - 2011/10/1

N2 - A Sri Lankan fever cohort (n = 292 patients; 17.8% prevalence) was used to assess two standard diagnostic Chikungunya IgM tests. The immunochromatographic test (ICT) acute sample sensitivity (SN) was 1.9 to 3.9%, and specificity (SP) was 92.5 to 95.0%. The enzyme-linked immunosorbent assay (ELISA) gave an acute sample SN of 3.9% and an SP of 92.5% and a convalescent sample SN of 84% and an SP of 91%. These assays are not suitable for the acute diagnosis of Chikungunya virus infection.

AB - A Sri Lankan fever cohort (n = 292 patients; 17.8% prevalence) was used to assess two standard diagnostic Chikungunya IgM tests. The immunochromatographic test (ICT) acute sample sensitivity (SN) was 1.9 to 3.9%, and specificity (SP) was 92.5 to 95.0%. The enzyme-linked immunosorbent assay (ELISA) gave an acute sample SN of 3.9% and an SP of 92.5% and a convalescent sample SN of 84% and an SP of 91%. These assays are not suitable for the acute diagnosis of Chikungunya virus infection.

U2 - 10.1128/cvi.05288-11

DO - 10.1128/cvi.05288-11

M3 - Article

SN - 1556-6811

VL - 18

SP - 1773

EP - 1775

JO - Clinical and Vaccine Immunology

JF - Clinical and Vaccine Immunology

IS - 10

ER -

Poor Diagnostic Accuracy of Commercial Antibody-Based Assays for the Diagnosis of Acute Chikungunya Infection

Abstract

Access to Document

Fingerprint

Cite this