Poor Diagnostic Accuracy of Commercial Antibody-Based Assays for the Diagnosis of Acute Chikungunya Infection

Stuart D. Blacksell; Ampai Tanganuchitcharnchai; Richard G. Jarman; Robert V. Gibbons; Daniel H. Paris; Mark S. Bailey; Nicholas P.J. Day; Ranjan Premaratna; David Lalloo; H. Janaka De Silva

doi:10.1128/cvi.05288-11

Poor Diagnostic Accuracy of Commercial Antibody-Based Assays for the Diagnosis of Acute Chikungunya Infection

Stuart D. Blacksell
, Ampai Tanganuchitcharnchai
, Richard G. Jarman
, Robert V. Gibbons
, Daniel H. Paris
, Mark S. Bailey
, Nicholas P.J. Day
, Ranjan Premaratna
, David Lalloo
, H. Janaka De Silva

Clinical Sciences

Research output: Contribution to journal › Article › peer-review

42 Citations (Scopus)

Abstract

A Sri Lankan fever cohort (n = 292 patients; 17.8% prevalence) was used to assess two standard diagnostic Chikungunya IgM tests. The immunochromatographic test (ICT) acute sample sensitivity (SN) was 1.9 to 3.9%, and specificity (SP) was 92.5 to 95.0%. The enzyme-linked immunosorbent assay (ELISA) gave an acute sample SN of 3.9% and an SP of 92.5% and a convalescent sample SN of 84% and an SP of 91%. These assays are not suitable for the acute diagnosis of Chikungunya virus infection.

Original language	English
Pages (from-to)	1773-1775
Number of pages	3
Journal	Clinical and Vaccine Immunology
Volume	18
Issue number	10
DOIs	https://doi.org/10.1128/cvi.05288-11
Publication status	Published - 1 Oct 2011

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Blacksell, S. D., Tanganuchitcharnchai, A., Jarman, R. G., Gibbons, R. V., Paris, D. H., Bailey, M. S., Day, N. P. J., Premaratna, R., Lalloo, D., & De Silva, H. J. (2011). Poor Diagnostic Accuracy of Commercial Antibody-Based Assays for the Diagnosis of Acute Chikungunya Infection. Clinical and Vaccine Immunology, 18(10), 1773-1775. https://doi.org/10.1128/cvi.05288-11

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title = "Poor Diagnostic Accuracy of Commercial Antibody-Based Assays for the Diagnosis of Acute Chikungunya Infection",

abstract = "A Sri Lankan fever cohort (n = 292 patients; 17.8\% prevalence) was used to assess two standard diagnostic Chikungunya IgM tests. The immunochromatographic test (ICT) acute sample sensitivity (SN) was 1.9 to 3.9\%, and specificity (SP) was 92.5 to 95.0\%. The enzyme-linked immunosorbent assay (ELISA) gave an acute sample SN of 3.9\% and an SP of 92.5\% and a convalescent sample SN of 84\% and an SP of 91\%. These assays are not suitable for the acute diagnosis of Chikungunya virus infection.",

author = "Blacksell, \{Stuart D.\} and Ampai Tanganuchitcharnchai and Jarman, \{Richard G.\} and Gibbons, \{Robert V.\} and Paris, \{Daniel H.\} and Bailey, \{Mark S.\} and Day, \{Nicholas P.J.\} and Ranjan Premaratna and David Lalloo and \{De Silva\}, \{H. Janaka\}",

year = "2011",

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doi = "10.1128/cvi.05288-11",

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AU - Blacksell, Stuart D.

AU - Tanganuchitcharnchai, Ampai

AU - Jarman, Richard G.

AU - Gibbons, Robert V.

AU - Paris, Daniel H.

AU - Bailey, Mark S.

AU - Day, Nicholas P.J.

AU - Premaratna, Ranjan

AU - Lalloo, David

AU - De Silva, H. Janaka

PY - 2011/10/1

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N2 - A Sri Lankan fever cohort (n = 292 patients; 17.8% prevalence) was used to assess two standard diagnostic Chikungunya IgM tests. The immunochromatographic test (ICT) acute sample sensitivity (SN) was 1.9 to 3.9%, and specificity (SP) was 92.5 to 95.0%. The enzyme-linked immunosorbent assay (ELISA) gave an acute sample SN of 3.9% and an SP of 92.5% and a convalescent sample SN of 84% and an SP of 91%. These assays are not suitable for the acute diagnosis of Chikungunya virus infection.

AB - A Sri Lankan fever cohort (n = 292 patients; 17.8% prevalence) was used to assess two standard diagnostic Chikungunya IgM tests. The immunochromatographic test (ICT) acute sample sensitivity (SN) was 1.9 to 3.9%, and specificity (SP) was 92.5 to 95.0%. The enzyme-linked immunosorbent assay (ELISA) gave an acute sample SN of 3.9% and an SP of 92.5% and a convalescent sample SN of 84% and an SP of 91%. These assays are not suitable for the acute diagnosis of Chikungunya virus infection.

U2 - 10.1128/cvi.05288-11

DO - 10.1128/cvi.05288-11

M3 - Article

SN - 1556-6811

VL - 18

SP - 1773

EP - 1775

JO - Clinical and Vaccine Immunology

JF - Clinical and Vaccine Immunology

IS - 10

ER -

Poor Diagnostic Accuracy of Commercial Antibody-Based Assays for the Diagnosis of Acute Chikungunya Infection

Abstract

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