Performance of the Xpert® MTB/RIF assay in an active case-finding strategy: a pilot study from Tanzania [Short communication]

Nyanda Elias Ntinginya; Bertie Squire; Kerry Millington; B. Mtafya; E. Saathoff; N. Heinrich; G. Rojas-Ponce; D. Kowuor; L. Maboko; K. Reither; P. Clowes; M. Hoelscher; A. Rachow

doi:10.5588/ijtld.12.0127

Performance of the Xpert® MTB/RIF assay in an active case-finding strategy: a pilot study from Tanzania [Short communication]

Nyanda Elias Ntinginya, Bertie Squire, Kerry Millington, B. Mtafya, E. Saathoff, N. Heinrich, G. Rojas-Ponce, D. Kowuor, L. Maboko, K. Reither, P. Clowes, M. Hoelscher, A. Rachow

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

In this pilot study, we evaluated the Xpert® MTB/RIF assay in an active case-finding strategy, using two spot sputum samples collected within a 1-hour interval from household contacts of smear-positive TB index cases. Tuberculosis (TB) confirmed by culture served as the reference standard. Among 219 enrolled contacts, the yield of active TB was 2.3%. While the sensitivity of smear microscopy was 60% (95%CI 14.7–94.7), Xpert MTB/RIF achieved a sensitivity of 100% (95%CI 47.81–100.0). All culture-confirmed cases tested positive by Xpert MTB/RIF on the first submitted sample, suggesting that the evaluation of only one sample could be sufficient for TB diagnosis in this context.

Original language	English
Pages (from-to)	1468-1470
Number of pages	3
Journal	International Journal of Tuberculosis and Lung Disease
Volume	16
Issue number	11
DOIs	https://doi.org/10.5588/ijtld.12.0127
Publication status	Published - 7 Sept 2012

Keywords

Household contacts
Index cases
Spot-spot sputum samples

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.5588/ijtld.12.0127

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Ntinginya, N. E., Squire, B., Millington, K., Mtafya, B., Saathoff, E., Heinrich, N., Rojas-Ponce, G., Kowuor, D., Maboko, L., Reither, K., Clowes, P., Hoelscher, M., & Rachow, A. (2012). Performance of the Xpert® MTB/RIF assay in an active case-finding strategy: a pilot study from Tanzania [Short communication]. International Journal of Tuberculosis and Lung Disease, 16(11), 1468-1470. https://doi.org/10.5588/ijtld.12.0127

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title = "Performance of the Xpert{\textregistered} MTB/RIF assay in an active case-finding strategy: a pilot study from Tanzania [Short communication]",

abstract = "In this pilot study, we evaluated the Xpert{\textregistered} MTB/RIF assay in an active case-finding strategy, using two spot sputum samples collected within a 1-hour interval from household contacts of smear-positive TB index cases. Tuberculosis (TB) confirmed by culture served as the reference standard. Among 219 enrolled contacts, the yield of active TB was 2.3\%. While the sensitivity of smear microscopy was 60\% (95\%CI 14.7–94.7), Xpert MTB/RIF achieved a sensitivity of 100\% (95\%CI 47.81–100.0). All culture-confirmed cases tested positive by Xpert MTB/RIF on the first submitted sample, suggesting that the evaluation of only one sample could be sufficient for TB diagnosis in this context.",

keywords = "Household contacts, Index cases, Spot-spot sputum samples",

author = "Ntinginya, \{Nyanda Elias\} and Bertie Squire and Kerry Millington and B. Mtafya and E. Saathoff and N. Heinrich and G. Rojas-Ponce and D. Kowuor and L. Maboko and K. Reither and P. Clowes and M. Hoelscher and A. Rachow",

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month = sep,

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doi = "10.5588/ijtld.12.0127",

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Ntinginya, NE, Squire, B , Millington, K, Mtafya, B, Saathoff, E, Heinrich, N, Rojas-Ponce, G, Kowuor, D, Maboko, L, Reither, K, Clowes, P, Hoelscher, M & Rachow, A 2012, 'Performance of the Xpert® MTB/RIF assay in an active case-finding strategy: a pilot study from Tanzania [Short communication]', International Journal of Tuberculosis and Lung Disease, vol. 16, no. 11, pp. 1468-1470. https://doi.org/10.5588/ijtld.12.0127

TY - JOUR

T1 - Performance of the Xpert® MTB/RIF assay in an active case-finding strategy: a pilot study from Tanzania [Short communication]

AU - Ntinginya, Nyanda Elias

AU - Squire, Bertie

AU - Millington, Kerry

AU - Mtafya, B.

AU - Saathoff, E.

AU - Heinrich, N.

AU - Rojas-Ponce, G.

AU - Kowuor, D.

AU - Maboko, L.

AU - Reither, K.

AU - Clowes, P.

AU - Hoelscher, M.

AU - Rachow, A.

PY - 2012/9/7

Y1 - 2012/9/7

N2 - In this pilot study, we evaluated the Xpert® MTB/RIF assay in an active case-finding strategy, using two spot sputum samples collected within a 1-hour interval from household contacts of smear-positive TB index cases. Tuberculosis (TB) confirmed by culture served as the reference standard. Among 219 enrolled contacts, the yield of active TB was 2.3%. While the sensitivity of smear microscopy was 60% (95%CI 14.7–94.7), Xpert MTB/RIF achieved a sensitivity of 100% (95%CI 47.81–100.0). All culture-confirmed cases tested positive by Xpert MTB/RIF on the first submitted sample, suggesting that the evaluation of only one sample could be sufficient for TB diagnosis in this context.

AB - In this pilot study, we evaluated the Xpert® MTB/RIF assay in an active case-finding strategy, using two spot sputum samples collected within a 1-hour interval from household contacts of smear-positive TB index cases. Tuberculosis (TB) confirmed by culture served as the reference standard. Among 219 enrolled contacts, the yield of active TB was 2.3%. While the sensitivity of smear microscopy was 60% (95%CI 14.7–94.7), Xpert MTB/RIF achieved a sensitivity of 100% (95%CI 47.81–100.0). All culture-confirmed cases tested positive by Xpert MTB/RIF on the first submitted sample, suggesting that the evaluation of only one sample could be sufficient for TB diagnosis in this context.

KW - Household contacts

KW - Index cases

KW - Spot-spot sputum samples

U2 - 10.5588/ijtld.12.0127

DO - 10.5588/ijtld.12.0127

M3 - Article

SN - 1027-3719

VL - 16

SP - 1468

EP - 1470

JO - International Journal of Tuberculosis and Lung Disease

JF - International Journal of Tuberculosis and Lung Disease

IS - 11

ER -

Performance of the Xpert® MTB/RIF assay in an active case-finding strategy: a pilot study from Tanzania [Short communication]

Abstract

Keywords

UN SDGs

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