Neospora caninum: oocyst challenge of pregnant cows

Alexander J. Trees; M. M. McAllister; C. S. Guy; J.W. McGarry; R. F. Smith; Diana J.L. Williams

doi:10.1016/s0304-4017(02)00234-0

Neospora caninum: oocyst challenge of pregnant cows

Alexander J. Trees, M. M. McAllister, C. S. Guy, J.W. McGarry, R. F. Smith, Diana J.L. Williams

Research output: Contribution to journal › Article › peer-review

68 Citations (Scopus)

Abstract

Three pregnant cows were each orally challenged at 10 weeks of gestation with 600 sporulated oocysts of Neospora caninum. The number of oocysts was limited by those available. In concurrent bioassays, one oocyst per os infected each of two gerbils. Challenged cattle developed Neospora-specific antibody, cell proliferation and gamma-interferon responses. N. caninum specific PCR demonstrated persisting infection in the brains of cows 4 months after calving. Abortion was not induced and there was no evidence of transplacental infection in the healthy calves born at full-term. This experiment suggests that the dose threshold for induction of abortion exceeds 600 oocysts. (C) 2002 Elsevier Science B.V. All rights reserved.

Original language	English
Pages (from-to)	147-154
Number of pages	8
Journal	Veterinary Parasitology
Volume	109
Issue number	1-2
Early online date	28 Aug 2002
DOIs	https://doi.org/10.1016/s0304-4017(02)00234-0
Publication status	E-pub ahead of print - 28 Aug 2002
Externally published	Yes

Keywords

Cattle
Gestation
Neospora caninum
Oocysts

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10.1016/s0304-4017(02)00234-0

Cite this

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title = "Neospora caninum: oocyst challenge of pregnant cows",

abstract = "Three pregnant cows were each orally challenged at 10 weeks of gestation with 600 sporulated oocysts of Neospora caninum. The number of oocysts was limited by those available. In concurrent bioassays, one oocyst per os infected each of two gerbils. Challenged cattle developed Neospora-specific antibody, cell proliferation and gamma-interferon responses. N. caninum specific PCR demonstrated persisting infection in the brains of cows 4 months after calving. Abortion was not induced and there was no evidence of transplacental infection in the healthy calves born at full-term. This experiment suggests that the dose threshold for induction of abortion exceeds 600 oocysts. (C) 2002 Elsevier Science B.V. All rights reserved.",

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T1 - Neospora caninum: oocyst challenge of pregnant cows

AU - Trees, Alexander J.

AU - McAllister, M. M.

AU - Guy, C. S.

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AU - Smith, R. F.

AU - Williams, Diana J.L.

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N2 - Three pregnant cows were each orally challenged at 10 weeks of gestation with 600 sporulated oocysts of Neospora caninum. The number of oocysts was limited by those available. In concurrent bioassays, one oocyst per os infected each of two gerbils. Challenged cattle developed Neospora-specific antibody, cell proliferation and gamma-interferon responses. N. caninum specific PCR demonstrated persisting infection in the brains of cows 4 months after calving. Abortion was not induced and there was no evidence of transplacental infection in the healthy calves born at full-term. This experiment suggests that the dose threshold for induction of abortion exceeds 600 oocysts. (C) 2002 Elsevier Science B.V. All rights reserved.

AB - Three pregnant cows were each orally challenged at 10 weeks of gestation with 600 sporulated oocysts of Neospora caninum. The number of oocysts was limited by those available. In concurrent bioassays, one oocyst per os infected each of two gerbils. Challenged cattle developed Neospora-specific antibody, cell proliferation and gamma-interferon responses. N. caninum specific PCR demonstrated persisting infection in the brains of cows 4 months after calving. Abortion was not induced and there was no evidence of transplacental infection in the healthy calves born at full-term. This experiment suggests that the dose threshold for induction of abortion exceeds 600 oocysts. (C) 2002 Elsevier Science B.V. All rights reserved.

KW - Cattle

KW - Gestation

KW - Neospora caninum

KW - Oocysts

U2 - 10.1016/s0304-4017(02)00234-0

DO - 10.1016/s0304-4017(02)00234-0

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VL - 109

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EP - 154

JO - Veterinary Parasitology

JF - Veterinary Parasitology

IS - 1-2

ER -

Neospora caninum: oocyst challenge of pregnant cows

Abstract

Keywords

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