Multiplex PCR to Differentiate Monkeypox Virus Clades

The International Severe Acute Respiratory and Emerging Infection Consortium Investigators; Christopher T. Williams; Alessandra Romero-Ramirez; Adeleye Adesola Semiu; Samuel Oluwafunmbi Ifabumuyi; Caitlin Greenland-Bews; Susan Gould; Dominic Wooding; Collette Allen; Anushri Somasundaran; Nicodemus Nnabuike Mkpuma; Dorcas Gado; Jolly Amoche Adole; Abdulakeem Eniola Amoo; Abisola Ajoke Adeyemi; Laure Bosquillon de Jarcy; Christine Goffinet; Jake Dunning; Malcolm G. Semple; Esto Bahizire; Afolabi Akinpelu; Thomas E. Fletcher; Ana I. Cubas-Atienzar; Cristina Leggio; Adeyinka Adedeji; Adesuyi A. Omoare; Thomas Edwards

doi:10.3201/eid3202.250686

Multiplex PCR to Differentiate Monkeypox Virus Clades

The International Severe Acute Respiratory and Emerging Infection Consortium Investigators
, Christopher T. Williams
, Alessandra Romero-Ramirez
, Adeleye Adesola Semiu
, Samuel Oluwafunmbi Ifabumuyi
, Caitlin Greenland-Bews
, Susan Gould
, Dominic Wooding
, Collette Allen
, Anushri Somasundaran
, Nicodemus Nnabuike Mkpuma
, Dorcas Gado
, Jolly Amoche Adole
, Abdulakeem Eniola Amoo
, Abisola Ajoke Adeyemi
, Laure Bosquillon de Jarcy
, Christine Goffinet
, Jake Dunning
, Malcolm G. Semple
, Esto Bahizire

Afolabi Akinpelu, Thomas E. Fletcher, Ana I. Cubas-Atienzar, Cristina Leggio, Adeyinka Adedeji, Adesuyi A. Omoare, Thomas Edwards

Liverpool School of Tropical Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

We designed a multiplex quantitative PCR to differentiate monkeypox virus clades. For clinical samples collected in the United Kingdom and Nigeria, sensitivity was 78% (95% CI 67.67%-86.14%) and specificity 94% (95% CI 80.84%-99.30%); for samples with cycle thresholds <35, sensitivity was 98% (95% CI 91.72%-99.96%) and specificity 94% (95% CI 80.84%-99.30%).

Original language	English
Pages (from-to)	250-254
Number of pages	5
Journal	Emerging Infectious Diseases
Volume	32
Issue number	2
DOIs	https://doi.org/10.3201/eid3202.250686
Publication status	Published - 28 Feb 2026

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

diagnostics
monkeypox virus
mpox
MPXV
Nigeria
PCR
United Kingdom
viruses
zoonoses

Themes

Emerging and Re-Emerging Diseases

Access to Document

10.3201/eid3202.250686

Williams et al mpox qPCR EIDAccepted author manuscript, 340 KBLicence: CC BY

Cite this

The International Severe Acute Respiratory and Emerging Infection Consortium Investigators, Williams, C. T., Romero-Ramirez, A., Semiu, A. A., Ifabumuyi, S. O., Greenland-Bews, C., Gould, S., Wooding, D., Allen, C., Somasundaran, A., Mkpuma, N. N., Gado, D., Adole, J. A., Amoo, A. E., Adeyemi, A. A., de Jarcy, L. B., Goffinet, C., Dunning, J., Semple, M. G., ... Edwards, T. (2026). Multiplex PCR to Differentiate Monkeypox Virus Clades. Emerging Infectious Diseases, 32(2), 250-254. https://doi.org/10.3201/eid3202.250686

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title = "Multiplex PCR to Differentiate Monkeypox Virus Clades",

abstract = "We designed a multiplex quantitative PCR to differentiate monkeypox virus clades. For clinical samples collected in the United Kingdom and Nigeria, sensitivity was 78\% (95\% CI 67.67\%-86.14\%) and specificity 94\% (95\% CI 80.84\%-99.30\%); for samples with cycle thresholds <35, sensitivity was 98\% (95\% CI 91.72\%-99.96\%) and specificity 94\% (95\% CI 80.84\%-99.30\%).",

keywords = "diagnostics, monkeypox virus, mpox, MPXV, Nigeria, PCR, United Kingdom, viruses, zoonoses",

author = "\{The International Severe Acute Respiratory and Emerging Infection Consortium Investigators\} and Williams, \{Christopher T.\} and Alessandra Romero-Ramirez and Semiu, \{Adeleye Adesola\} and Ifabumuyi, \{Samuel Oluwafunmbi\} and Caitlin Greenland-Bews and Susan Gould and Dominic Wooding and Collette Allen and Anushri Somasundaran and Mkpuma, \{Nicodemus Nnabuike\} and Dorcas Gado and Adole, \{Jolly Amoche\} and Amoo, \{Abdulakeem Eniola\} and Adeyemi, \{Abisola Ajoke\} and \{de Jarcy\}, \{Laure Bosquillon\} and Christine Goffinet and Jake Dunning and Semple, \{Malcolm G.\} and Esto Bahizire and Afolabi Akinpelu and Fletcher, \{Thomas E.\} and Cubas-Atienzar, \{Ana I.\} and Cristina Leggio and Adeyinka Adedeji and Omoare, \{Adesuyi A.\} and Thomas Edwards",

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doi = "10.3201/eid3202.250686",

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The International Severe Acute Respiratory and Emerging Infection Consortium Investigators, Williams, CT, Romero-Ramirez, A, Semiu, AA, Ifabumuyi, SO, Greenland-Bews, C , Gould, S , Wooding, D, Allen, C, Somasundaran, A, Mkpuma, NN, Gado, D, Adole, JA, Amoo, AE, Adeyemi, AA, de Jarcy, LB, Goffinet, C, Dunning, J, Semple, MG, Bahizire, E, Akinpelu, A, Fletcher, TE , Cubas-Atienzar, AI, Leggio, C, Adedeji, A, Omoare, AA & Edwards, T 2026, 'Multiplex PCR to Differentiate Monkeypox Virus Clades', Emerging Infectious Diseases, vol. 32, no. 2, pp. 250-254. https://doi.org/10.3201/eid3202.250686

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T1 - Multiplex PCR to Differentiate Monkeypox Virus Clades

AU - The International Severe Acute Respiratory and Emerging Infection Consortium Investigators

AU - Williams, Christopher T.

AU - Romero-Ramirez, Alessandra

AU - Semiu, Adeleye Adesola

AU - Ifabumuyi, Samuel Oluwafunmbi

AU - Greenland-Bews, Caitlin

AU - Gould, Susan

AU - Wooding, Dominic

AU - Allen, Collette

AU - Somasundaran, Anushri

AU - Mkpuma, Nicodemus Nnabuike

AU - Gado, Dorcas

AU - Adole, Jolly Amoche

AU - Amoo, Abdulakeem Eniola

AU - Adeyemi, Abisola Ajoke

AU - de Jarcy, Laure Bosquillon

AU - Goffinet, Christine

AU - Dunning, Jake

AU - Semple, Malcolm G.

AU - Bahizire, Esto

AU - Akinpelu, Afolabi

AU - Fletcher, Thomas E.

AU - Cubas-Atienzar, Ana I.

AU - Leggio, Cristina

AU - Adedeji, Adeyinka

AU - Omoare, Adesuyi A.

AU - Edwards, Thomas

PY - 2026/2/28

Y1 - 2026/2/28

N2 - We designed a multiplex quantitative PCR to differentiate monkeypox virus clades. For clinical samples collected in the United Kingdom and Nigeria, sensitivity was 78% (95% CI 67.67%-86.14%) and specificity 94% (95% CI 80.84%-99.30%); for samples with cycle thresholds <35, sensitivity was 98% (95% CI 91.72%-99.96%) and specificity 94% (95% CI 80.84%-99.30%).

AB - We designed a multiplex quantitative PCR to differentiate monkeypox virus clades. For clinical samples collected in the United Kingdom and Nigeria, sensitivity was 78% (95% CI 67.67%-86.14%) and specificity 94% (95% CI 80.84%-99.30%); for samples with cycle thresholds <35, sensitivity was 98% (95% CI 91.72%-99.96%) and specificity 94% (95% CI 80.84%-99.30%).

KW - diagnostics

KW - monkeypox virus

KW - mpox

KW - MPXV

KW - Nigeria

KW - PCR

KW - United Kingdom

KW - viruses

KW - zoonoses

U2 - 10.3201/eid3202.250686

DO - 10.3201/eid3202.250686

M3 - Article

C2 - 41663215

AN - SCOPUS:105030762339

SN - 1080-6040

VL - 32

SP - 250

EP - 254

JO - Emerging Infectious Diseases

JF - Emerging Infectious Diseases

IS - 2

ER -

Multiplex PCR to Differentiate Monkeypox Virus Clades

Abstract

UN SDGs

Keywords

Themes

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