Mosquito transgenic technologies to reduce plasmodium transmission

Silke Fuchs; Tony Nolan; Andrea Crisanti

doi:10.1007/978-1-62703-26-7_41

Mosquito transgenic technologies to reduce plasmodium transmission

Silke Fuchs, Tony Nolan, Andrea Crisanti

Liverpool School of Tropical Medicine

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

39 Citations (Scopus)

Abstract

The ability to introduce genetic constructs of choice into the genome of Anopheles mosquitoes provides a valuable tool to study the molecular interactions between the Plasmodium parasite and its insect host. In the long term, this technology could potentially offer new ways to control vector-borne diseases through the suppression of target mosquito populations or through the introgression of traits that preclude pathogen transmission. Here, we describe in detail protocols for the generation of transgenic Anopheles gambiae mosquitoes based on germ-line transformation using either modified transposable elements or the sitespecific PhiC31 recombinase .

Original language	English
Title of host publication	Methods in Molecular Biology
Pages	601-622
Number of pages	22
DOIs	https://doi.org/10.1007/978-1-62703-26-7_41
Publication status	Published - 1 Jan 2013

Keywords

Effector gene
Embryo injection
Genetic modification
Mosquito
PhiC31
PiggyBac
Plasmodium
Transgenic
Transposable element

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10.1007/978-1-62703-26-7_41

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KW - Embryo injection

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KW - Mosquito

KW - PhiC31

KW - PiggyBac

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KW - Transgenic

KW - Transposable element

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Mosquito transgenic technologies to reduce plasmodium transmission

Abstract

Keywords

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