Molecular cloning of a gene encoding a 20S proteasome β subunit from Plasmodium falciparum

Gao De Li; Ji Liang Li; Mathirut Mugthin; Steve Ward

doi:10.1016/s0020-7519(00)00046-1

Molecular cloning of a gene encoding a 20S proteasome β subunit from Plasmodium falciparum

Gao De Li, Ji Liang Li, Mathirut Mugthin, Steve Ward

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

A novel gene was cloned from Plasmodium falciparum. Database searches indicated this gene to be a member of the 20S proteasome β-subunit family. Comparison of the gene's genomic DNA sequence with cDNA sequence revealed a 156-bp intron 85 bp downstream from the start codon. The nucleotide sequence of the gene contains one open reading frame encoding 265 amino acids with a predicted molecular mass of 30.9 kDa and a pI of 6.2. Northern blot analysis showed the transcript size to be approximately 1.6 kb indicating that some 800 bp of the transcript is non-coding. (C) 2000 Australian Society for Parasitology Inc.

Original language	English
Pages (from-to)	729-733
Number of pages	5
Journal	International Journal for Parasitology
Volume	30
Issue number	6
DOIs	https://doi.org/10.1016/s0020-7519(00)00046-1
Publication status	Published - 1 May 2000
Externally published	Yes

Keywords

Beta-subunit
Multicatalytic protease complex
Plasmodium falciparum
Proteasome

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10.1016/s0020-7519(00)00046-1

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title = "Molecular cloning of a gene encoding a 20S proteasome β subunit from Plasmodium falciparum",

abstract = "A novel gene was cloned from Plasmodium falciparum. Database searches indicated this gene to be a member of the 20S proteasome β-subunit family. Comparison of the gene's genomic DNA sequence with cDNA sequence revealed a 156-bp intron 85 bp downstream from the start codon. The nucleotide sequence of the gene contains one open reading frame encoding 265 amino acids with a predicted molecular mass of 30.9 kDa and a pI of 6.2. Northern blot analysis showed the transcript size to be approximately 1.6 kb indicating that some 800 bp of the transcript is non-coding. (C) 2000 Australian Society for Parasitology Inc.",

keywords = "Beta-subunit, Multicatalytic protease complex, Plasmodium falciparum, Proteasome",

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year = "2000",

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doi = "10.1016/s0020-7519(00)00046-1",

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T1 - Molecular cloning of a gene encoding a 20S proteasome β subunit from Plasmodium falciparum

AU - Li, Gao De

AU - Li, Ji Liang

AU - Mugthin, Mathirut

AU - Ward, Steve

PY - 2000/5/1

Y1 - 2000/5/1

N2 - A novel gene was cloned from Plasmodium falciparum. Database searches indicated this gene to be a member of the 20S proteasome β-subunit family. Comparison of the gene's genomic DNA sequence with cDNA sequence revealed a 156-bp intron 85 bp downstream from the start codon. The nucleotide sequence of the gene contains one open reading frame encoding 265 amino acids with a predicted molecular mass of 30.9 kDa and a pI of 6.2. Northern blot analysis showed the transcript size to be approximately 1.6 kb indicating that some 800 bp of the transcript is non-coding. (C) 2000 Australian Society for Parasitology Inc.

AB - A novel gene was cloned from Plasmodium falciparum. Database searches indicated this gene to be a member of the 20S proteasome β-subunit family. Comparison of the gene's genomic DNA sequence with cDNA sequence revealed a 156-bp intron 85 bp downstream from the start codon. The nucleotide sequence of the gene contains one open reading frame encoding 265 amino acids with a predicted molecular mass of 30.9 kDa and a pI of 6.2. Northern blot analysis showed the transcript size to be approximately 1.6 kb indicating that some 800 bp of the transcript is non-coding. (C) 2000 Australian Society for Parasitology Inc.

KW - Beta-subunit

KW - Multicatalytic protease complex

KW - Plasmodium falciparum

KW - Proteasome

U2 - 10.1016/s0020-7519(00)00046-1

DO - 10.1016/s0020-7519(00)00046-1

M3 - Article

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VL - 30

SP - 729

EP - 733

JO - International Journal for Parasitology

JF - International Journal for Parasitology

IS - 6

ER -

Molecular cloning of a gene encoding a 20S proteasome β subunit from Plasmodium falciparum

Abstract

Keywords

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