Molecular analysis of multiple cytochrome P450 genes from the malaria vector, Anopheles gambiae

Hilary Ranson; D. Nikou; M. Hutchinson; X. Wang; C. W. Roth; Janet Hemingway; F. H. Collins

doi:10.1046/j.1365-2583.2002.00350.x

Molecular analysis of multiple cytochrome P450 genes from the malaria vector, Anopheles gambiae

Hilary Ranson, D. Nikou, M. Hutchinson, X. Wang, C. W. Roth, Janet Hemingway, F. H. Collins

Vector Biology

Research output: Contribution to journal › Article › peer-review

59 Citations (Scopus)

Abstract

Cytochrome P450s are a superfamily of haemoproteins, important in the metabolism of endogenous compounds and xenobiotics. As a first step to elucidating the role of this family in insecticide resistance in the malaria mosquito, Anopheles gambiae , we have cloned and mapped multiple P450 genes. Sixteen cDNAs encoding full-length P450s were cloned and physically mapped to the mosquito's polytene chromosomes. Fourteen of these encode putative CYP6 proteins and two encode P450s belonging to the CYP9 class. Eighteen new A. gambiae Cyp4 P450 genes were identified using degenerate PCR primers, cDNAs were detected for ten and in situ locations for thirteen members of this gene family.

Original language	English
Pages (from-to)	409-418
Number of pages	10
Journal	Insect Molecular Biology
Volume	11
Issue number	5
DOIs	https://doi.org/10.1046/j.1365-2583.2002.00350.x
Publication status	Published - 1 Oct 2002

Keywords

Gene clusters
Insecticide resistance
Monooxygenases
Mosquito
Polytene chromosomes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1046/j.1365-2583.2002.00350.x

Cite this

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title = "Molecular analysis of multiple cytochrome P450 genes from the malaria vector, Anopheles gambiae",

abstract = "Cytochrome P450s are a superfamily of haemoproteins, important in the metabolism of endogenous compounds and xenobiotics. As a first step to elucidating the role of this family in insecticide resistance in the malaria mosquito, Anopheles gambiae , we have cloned and mapped multiple P450 genes. Sixteen cDNAs encoding full-length P450s were cloned and physically mapped to the mosquito's polytene chromosomes. Fourteen of these encode putative CYP6 proteins and two encode P450s belonging to the CYP9 class. Eighteen new A. gambiae Cyp4 P450 genes were identified using degenerate PCR primers, cDNAs were detected for ten and in situ locations for thirteen members of this gene family.",

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T1 - Molecular analysis of multiple cytochrome P450 genes from the malaria vector, Anopheles gambiae

AU - Ranson, Hilary

AU - Nikou, D.

AU - Hutchinson, M.

AU - Wang, X.

AU - Roth, C. W.

AU - Hemingway, Janet

AU - Collins, F. H.

PY - 2002/10/1

Y1 - 2002/10/1

N2 - Cytochrome P450s are a superfamily of haemoproteins, important in the metabolism of endogenous compounds and xenobiotics. As a first step to elucidating the role of this family in insecticide resistance in the malaria mosquito, Anopheles gambiae , we have cloned and mapped multiple P450 genes. Sixteen cDNAs encoding full-length P450s were cloned and physically mapped to the mosquito's polytene chromosomes. Fourteen of these encode putative CYP6 proteins and two encode P450s belonging to the CYP9 class. Eighteen new A. gambiae Cyp4 P450 genes were identified using degenerate PCR primers, cDNAs were detected for ten and in situ locations for thirteen members of this gene family.

AB - Cytochrome P450s are a superfamily of haemoproteins, important in the metabolism of endogenous compounds and xenobiotics. As a first step to elucidating the role of this family in insecticide resistance in the malaria mosquito, Anopheles gambiae , we have cloned and mapped multiple P450 genes. Sixteen cDNAs encoding full-length P450s were cloned and physically mapped to the mosquito's polytene chromosomes. Fourteen of these encode putative CYP6 proteins and two encode P450s belonging to the CYP9 class. Eighteen new A. gambiae Cyp4 P450 genes were identified using degenerate PCR primers, cDNAs were detected for ten and in situ locations for thirteen members of this gene family.

KW - Gene clusters

KW - Insecticide resistance

KW - Monooxygenases

KW - Mosquito

KW - Polytene chromosomes

U2 - 10.1046/j.1365-2583.2002.00350.x

DO - 10.1046/j.1365-2583.2002.00350.x

M3 - Article

SN - 0962-1075

VL - 11

SP - 409

EP - 418

JO - Insect Molecular Biology

JF - Insect Molecular Biology

IS - 5

ER -

Molecular analysis of multiple cytochrome P450 genes from the malaria vector, Anopheles gambiae

Abstract

Keywords

UN SDGs

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