Midgut transcriptomic responses to dengue and chikungunya viruses in the vectors Aedes albopictus and Aedes malayensis

Cassie Modahl; Avisha Chowdhury; Dolyce H.W. Low; Menchie C. Manuel; Dorothée Missé; R. Manjunatha Kini; Ian H. Mendenhall; Julien Pompon

doi:10.1038/s41598-023-38354-9

Midgut transcriptomic responses to dengue and chikungunya viruses in the vectors Aedes albopictus and Aedes malayensis

Cassie Modahl, Avisha Chowdhury, Dolyce H.W. Low, Menchie C. Manuel, Dorothée Missé, R. Manjunatha Kini, Ian H. Mendenhall, Julien Pompon

Tropical Disease Biology

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Dengue (DENV) and chikungunya (CHIKV) viruses are among the most preponderant arboviruses. Although primarily transmitted through the bite of Aedes aegypti mosquitoes, Aedes albopictus and Aedes malayensis are competent vectors and have an impact on arbovirus epidemiology. Here, to fill the gap in our understanding of the molecular interactions between secondary vectors and arboviruses, we used transcriptomics to profile the whole-genome responses of A. albopictus to CHIKV and of A. malayensis to CHIKV and DENV at 1 and 4 days post-infection (dpi) in midguts. In A. albopictus, 1793 and 339 genes were significantly regulated by CHIKV at 1 and 4 dpi, respectively. In A. malayensis, 943 and 222 genes upon CHIKV infection, and 74 and 69 genes upon DENV infection were significantly regulated at 1 and 4 dpi, respectively. We reported 81 genes that were consistently differentially regulated in all the CHIKV-infected conditions, identifying a CHIKV-induced signature. We identified expressed immune genes in both mosquito species, using a de novo assembled midgut transcriptome for A. malayensis, and described the immune architectures. We found the JNK pathway activated in all conditions, generalizing its antiviral function to Aedines. Our comprehensive study provides insight into arbovirus transmission by multiple Aedes vectors.

Original language	English
Article number	11271
Pages (from-to)	e11271
Journal	Scientific Reports
Volume	13
Issue number	1
DOIs	https://doi.org/10.1038/s41598-023-38354-9
Publication status	Published - 12 Jul 2023

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title = "Midgut transcriptomic responses to dengue and chikungunya viruses in the vectors Aedes albopictus and Aedes malayensis",

abstract = "Dengue (DENV) and chikungunya (CHIKV) viruses are among the most preponderant arboviruses. Although primarily transmitted through the bite of Aedes aegypti mosquitoes, Aedes albopictus and Aedes malayensis are competent vectors and have an impact on arbovirus epidemiology. Here, to fill the gap in our understanding of the molecular interactions between secondary vectors and arboviruses, we used transcriptomics to profile the whole-genome responses of A. albopictus to CHIKV and of A. malayensis to CHIKV and DENV at 1 and 4 days post-infection (dpi) in midguts. In A. albopictus, 1793 and 339 genes were significantly regulated by CHIKV at 1 and 4 dpi, respectively. In A. malayensis, 943 and 222 genes upon CHIKV infection, and 74 and 69 genes upon DENV infection were significantly regulated at 1 and 4 dpi, respectively. We reported 81 genes that were consistently differentially regulated in all the CHIKV-infected conditions, identifying a CHIKV-induced signature. We identified expressed immune genes in both mosquito species, using a de novo assembled midgut transcriptome for A. malayensis, and described the immune architectures. We found the JNK pathway activated in all conditions, generalizing its antiviral function to Aedines. Our comprehensive study provides insight into arbovirus transmission by multiple Aedes vectors.",

author = "Cassie Modahl and Avisha Chowdhury and Low, \{Dolyce H.W.\} and Manuel, \{Menchie C.\} and Doroth{\'e}e Miss{\'e} and Kini, \{R. Manjunatha\} and Mendenhall, \{Ian H.\} and Julien Pompon",

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AU - Modahl, Cassie

AU - Chowdhury, Avisha

AU - Low, Dolyce H.W.

AU - Manuel, Menchie C.

AU - Missé, Dorothée

AU - Kini, R. Manjunatha

AU - Mendenhall, Ian H.

AU - Pompon, Julien

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DO - 10.1038/s41598-023-38354-9

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Midgut transcriptomic responses to dengue and chikungunya viruses in the vectors Aedes albopictus and Aedes malayensis

Abstract

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