TY - JOUR
T1 - Microscopic observation drug susceptibility assay (MODS) for early diagnosis of tuberculosis in children
AU - Ha, Dang Thi Minh
AU - Lan, Nguyen Thi Ngoc
AU - Wolbers, Marcel
AU - Duong, Tran Ngoc
AU - Quang, Nguyen Dang
AU - van Thinh, Tran Thi
AU - Ngoc, Le Thi Hong
AU - Anh, Nguyen Thi Ngoc
AU - van Quyet, Tran
AU - Tuyen, Nguyen Thi Bich
AU - Ha, Vo Thi
AU - Day, Jeremy
AU - Hoang, Thi Thanh Hang
AU - Kiet, Vo Sy
AU - Nho, Nguyen Thi
AU - Hoa, Dai Viet
AU - Dung, Nguyen Huy
AU - Lan, Nguyen Huu
AU - Farrar, Jeremy
AU - Caws, Maxine
PY - 2009/1/1
Y1 - 2009/1/1
N2 - MODS is a novel liquid culture based technique that has been shown to be effective and rapid for early diagnosis of tuberculosis (TB). We evaluated the MODS assay for diagnosis of TB in children in Viet Nam. 217 consecutive samples including sputum (n=132), gastric fluid (n=50), CSF (n= 32) and pleural fluid (n=3) collected from 96 children with suspected TB, were tested by smear, MODS and MGIT. When test results were aggregated by patient, the sensitivity and specificity of smear, MGIT and MODS against ''clinical diagnosis'' (confirmed and probable groups) as the gold standard were 28.2% and 100%, 42.3% and 100%, 39.7% and 94.4%, respectively. The sensitivity of MGIT and MODS was not significantly different in this analysis (P= 0.5), but MGIT was more sensitive than MODS when analysed on the sample level using a marginal model (P =0.03). The median time to detection of MODS and MGIT were 8 days and 13 days, respectively, and the time to detection was significantly shorter for MODS in samples where both tests were positive (P,0.001). An analysis of time-dependent sensitivity showed that the detection rates were significantly higher for MODS than for MGIT by day 7 or day 14 (P,0.001 and P =0.04), respectively. MODS is a rapid and sensitive alternative method for the isolation of M.tuberculosis from children.
AB - MODS is a novel liquid culture based technique that has been shown to be effective and rapid for early diagnosis of tuberculosis (TB). We evaluated the MODS assay for diagnosis of TB in children in Viet Nam. 217 consecutive samples including sputum (n=132), gastric fluid (n=50), CSF (n= 32) and pleural fluid (n=3) collected from 96 children with suspected TB, were tested by smear, MODS and MGIT. When test results were aggregated by patient, the sensitivity and specificity of smear, MGIT and MODS against ''clinical diagnosis'' (confirmed and probable groups) as the gold standard were 28.2% and 100%, 42.3% and 100%, 39.7% and 94.4%, respectively. The sensitivity of MGIT and MODS was not significantly different in this analysis (P= 0.5), but MGIT was more sensitive than MODS when analysed on the sample level using a marginal model (P =0.03). The median time to detection of MODS and MGIT were 8 days and 13 days, respectively, and the time to detection was significantly shorter for MODS in samples where both tests were positive (P,0.001). An analysis of time-dependent sensitivity showed that the detection rates were significantly higher for MODS than for MGIT by day 7 or day 14 (P,0.001 and P =0.04), respectively. MODS is a rapid and sensitive alternative method for the isolation of M.tuberculosis from children.
U2 - 10.1371/journal.pone.0008341
DO - 10.1371/journal.pone.0008341
M3 - Article
VL - 4
JO - PLoS ONE
JF - PLoS ONE
IS - 12
M1 - e8341
ER -
