Mass spectrometry identification of age-associated proteins from the malaria mosquitoes Anopheles gambiae s.s. and Anopheles stephensi

Maggy T. Sikulu; James Monkman; Keyur A. Dave; Marcus L. Hastie; Patricia E. Dale; Roger L. Kitching; Gerry Killeen; Brian H. Kay; Jeffry J. Gorman; Leon E. Hugo

doi:10.1016/j.dib.2015.07.007

Mass spectrometry identification of age-associated proteins from the malaria mosquitoes Anopheles gambiae s.s. and Anopheles stephensi

Maggy T. Sikulu, James Monkman, Keyur A. Dave, Marcus L. Hastie, Patricia E. Dale, Roger L. Kitching, Gerry Killeen, Brian H. Kay, Jeffry J. Gorman, Leon E. Hugo

Vector Biology

Liverpool School of Tropical Medicine

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

This study investigated proteomic changes occurring in Anopheles gambiae and Anopheles stephensi during adult mosquito aging. These changes were evaluated using two-dimensional difference gel electrophoresis (2D-DIGE) and the identities of aging related proteins were determined using capillary high-pressure liquid chromatography (capHPLC) coupled with a linear ion-trap (LTQ)-Orbitrap XL hybrid mass spectrometry (MS). Here, we have described the techniques used to determine age associated proteomic changes occurring in heads and thoraces across three age groups; 1, 9 and 17 d old A. gambiae and 4 age groups; 1, 9, 17 and 34 d old A. stephensi. We have provided normalised spot volume raw data for all protein spots that were visible on 2D-DIGE images for both species and processed Orbitrap mass spectrometry data. For public access, mass spectrometry raw data are available via ProteomeXchange with identifier PXD002153. A detailed description of this study has been described elsewhere [1].

Original language	English
Pages (from-to)	461-467
Number of pages	7
Journal	Data in Brief
Volume	4
DOIs	https://doi.org/10.1016/j.dib.2015.07.007
Publication status	Published - 16 Jul 2015

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Data Brief 4 461-467Final published version, 3.03 MBLicence: CC BY

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@article{82fe6ddae63946d4ae8f8582bb205299,

title = "Mass spectrometry identification of age-associated proteins from the malaria mosquitoes Anopheles gambiae s.s. and Anopheles stephensi",

abstract = "This study investigated proteomic changes occurring in Anopheles gambiae and Anopheles stephensi during adult mosquito aging. These changes were evaluated using two-dimensional difference gel electrophoresis (2D-DIGE) and the identities of aging related proteins were determined using capillary high-pressure liquid chromatography (capHPLC) coupled with a linear ion-trap (LTQ)-Orbitrap XL hybrid mass spectrometry (MS). Here, we have described the techniques used to determine age associated proteomic changes occurring in heads and thoraces across three age groups; 1, 9 and 17 d old A. gambiae and 4 age groups; 1, 9, 17 and 34 d old A. stephensi. We have provided normalised spot volume raw data for all protein spots that were visible on 2D-DIGE images for both species and processed Orbitrap mass spectrometry data. For public access, mass spectrometry raw data are available via ProteomeXchange with identifier PXD002153. A detailed description of this study has been described elsewhere [1].",

author = "Sikulu, \{Maggy T.\} and James Monkman and Dave, \{Keyur A.\} and Hastie, \{Marcus L.\} and Dale, \{Patricia E.\} and Kitching, \{Roger L.\} and Gerry Killeen and Kay, \{Brian H.\} and Gorman, \{Jeffry J.\} and Hugo, \{Leon E.\}",

year = "2015",

month = jul,

day = "16",

doi = "10.1016/j.dib.2015.07.007",

language = "English",

volume = "4",

pages = "461--467",

journal = "Data in Brief",

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T1 - Mass spectrometry identification of age-associated proteins from the malaria mosquitoes Anopheles gambiae s.s. and Anopheles stephensi

AU - Sikulu, Maggy T.

AU - Monkman, James

AU - Dave, Keyur A.

AU - Hastie, Marcus L.

AU - Dale, Patricia E.

AU - Kitching, Roger L.

AU - Killeen, Gerry

AU - Kay, Brian H.

AU - Gorman, Jeffry J.

AU - Hugo, Leon E.

PY - 2015/7/16

Y1 - 2015/7/16

N2 - This study investigated proteomic changes occurring in Anopheles gambiae and Anopheles stephensi during adult mosquito aging. These changes were evaluated using two-dimensional difference gel electrophoresis (2D-DIGE) and the identities of aging related proteins were determined using capillary high-pressure liquid chromatography (capHPLC) coupled with a linear ion-trap (LTQ)-Orbitrap XL hybrid mass spectrometry (MS). Here, we have described the techniques used to determine age associated proteomic changes occurring in heads and thoraces across three age groups; 1, 9 and 17 d old A. gambiae and 4 age groups; 1, 9, 17 and 34 d old A. stephensi. We have provided normalised spot volume raw data for all protein spots that were visible on 2D-DIGE images for both species and processed Orbitrap mass spectrometry data. For public access, mass spectrometry raw data are available via ProteomeXchange with identifier PXD002153. A detailed description of this study has been described elsewhere [1].

AB - This study investigated proteomic changes occurring in Anopheles gambiae and Anopheles stephensi during adult mosquito aging. These changes were evaluated using two-dimensional difference gel electrophoresis (2D-DIGE) and the identities of aging related proteins were determined using capillary high-pressure liquid chromatography (capHPLC) coupled with a linear ion-trap (LTQ)-Orbitrap XL hybrid mass spectrometry (MS). Here, we have described the techniques used to determine age associated proteomic changes occurring in heads and thoraces across three age groups; 1, 9 and 17 d old A. gambiae and 4 age groups; 1, 9, 17 and 34 d old A. stephensi. We have provided normalised spot volume raw data for all protein spots that were visible on 2D-DIGE images for both species and processed Orbitrap mass spectrometry data. For public access, mass spectrometry raw data are available via ProteomeXchange with identifier PXD002153. A detailed description of this study has been described elsewhere [1].

U2 - 10.1016/j.dib.2015.07.007

DO - 10.1016/j.dib.2015.07.007

M3 - Article

SN - 2352-3409

VL - 4

SP - 461

EP - 467

JO - Data in Brief

JF - Data in Brief

ER -

Mass spectrometry identification of age-associated proteins from the malaria mosquitoes Anopheles gambiae s.s. and Anopheles stephensi

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