MAIT cells are activated during human viral infections

Bonnie Van Wilgenburg; Iris Scherwitzl; Edward C. Hutchinson; Tianqi Leng; Ayako Kurioka; Corinna Kulicke; Catherine De Lara; Suzanne Cole; Sirijitt Vasanawathana; Wannee Limpitikul; Prida Malasit; Duncan Young; Laura Denney; Michael D. Moore; Paolo Fabris; Maria Teresa Giordani; Ye Htun Oo; Stephen M. Laidlaw; Lynn B. Dustin; Ling Pei Ho; Fiona M. Thompson; Narayan Ramamurthy; Juthathip Mongkolsapaya; Christian B. Willberg; Gavin R. Screaton; Paul Klenerman; Eleanor Barnes; Jonathan Ball; Gary Burgess; Graham Cooke; John Dillon; Charles Gore; Graham Foster; Neil Guha; Rachel Halford; Cham Herath; Chris Holmes; Anita Howe; Emma Hudson; William Irving; Salim Khakoo; Diana Koletzki; Natasha Martin; Tamyo Mbisa; Jane McKeating; John McLauchlan; Alec Miners; Andrea Murray; Peter Shaw; Peter Simmonds

doi:10.1038/ncomms11653

MAIT cells are activated during human viral infections

Bonnie Van Wilgenburg
, Iris Scherwitzl
, Edward C. Hutchinson
, Tianqi Leng
, Ayako Kurioka
, Corinna Kulicke
, Catherine De Lara
, Suzanne Cole
, Sirijitt Vasanawathana
, Wannee Limpitikul
, Prida Malasit
, Duncan Young
, Laura Denney
, Michael D. Moore
, Paolo Fabris
, Maria Teresa Giordani
, Ye Htun Oo
, Stephen M. Laidlaw
, Lynn B. Dustin
, Ling Pei Ho

Fiona M. Thompson, Narayan Ramamurthy, Juthathip Mongkolsapaya, Christian B. Willberg, Gavin R. Screaton, Paul Klenerman, Eleanor Barnes, Jonathan Ball, Gary Burgess, Graham Cooke, John Dillon, Charles Gore, Graham Foster, Neil Guha, Rachel Halford, Cham Herath, Chris Holmes, Anita Howe, Emma Hudson, William Irving, Salim Khakoo, Diana Koletzki, Natasha Martin, Tamyo Mbisa, Jane McKeating, John McLauchlan, Alec Miners, Andrea Murray, Peter Shaw, Peter Simmonds

University of Oxford
Imperial College London
Thailand Ministry of Public Health
National Science and Technology Development Agency Thailand
Mahidol University
John Radcliffe Hospital
Azienda Sanitaria Ulss 6 Vicenza
University of Birmingham
University of Nottingham
Histogen, Inc.
University of Dundee
Hepatitis C Trust
Queen Mary University of London
Gilead Sciences
Providence Health Care Canada
University of Southampton
Johnson & Johnson
University of California at San Diego
UK Health Security Agency
University of Glasgow
London School of Hygiene and Tropical Medicine
City Hospital Campus
Merck

Research output: Contribution to journal › Article › peer-review

442 Citations (Scopus)

Abstract

Mucosal-associated invariant T (MAIT) cells are abundant in humans and recognize bacterial ligands. Here, we demonstrate that MAIT cells are also activated during human viral infections in vivo. MAIT cells activation was observed during infection with dengue virus, hepatitis C virus and influenza virus. This activation - driving cytokine release and Granzyme B upregulation - is TCR-independent but dependent on IL-18 in synergy with IL-12, IL-15 and/or interferon-α/β. IL-18 levels and MAIT cell activation correlate with disease severity in acute dengue infection. Furthermore, HCV treatment with interferon-α leads to specific MAIT cell activation in vivo in parallel with an enhanced therapeutic response. Moreover, TCR-independent activation of MAIT cells leads to a reduction of HCV replication in vitro mediated by IFN-γ. Together these data demonstrate MAIT cells are activated following viral infections, and suggest a potential role in both host defence and immunopathology.

Original language	English
Article number	11653
Journal	Nature Communications
Volume	7
DOIs	https://doi.org/10.1038/ncomms11653
Publication status	Published - 23 Jun 2016
Externally published	Yes

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SDG 3 Good Health and Well-being

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Van Wilgenburg, B., Scherwitzl, I., Hutchinson, E. C., Leng, T., Kurioka, A., Kulicke, C., De Lara, C., Cole, S., Vasanawathana, S., Limpitikul, W., Malasit, P., Young, D., Denney, L., Moore, M. D., Fabris, P., Giordani, M. T., Oo, Y. H., Laidlaw, S. M., Dustin, L. B., ... Simmonds, P. (2016). MAIT cells are activated during human viral infections. Nature Communications, 7, Article 11653. https://doi.org/10.1038/ncomms11653

@article{6888fc569e7342c6ba7cec566850bac8,

title = "MAIT cells are activated during human viral infections",

abstract = "Mucosal-associated invariant T (MAIT) cells are abundant in humans and recognize bacterial ligands. Here, we demonstrate that MAIT cells are also activated during human viral infections in vivo. MAIT cells activation was observed during infection with dengue virus, hepatitis C virus and influenza virus. This activation - driving cytokine release and Granzyme B upregulation - is TCR-independent but dependent on IL-18 in synergy with IL-12, IL-15 and/or interferon-α/β. IL-18 levels and MAIT cell activation correlate with disease severity in acute dengue infection. Furthermore, HCV treatment with interferon-α leads to specific MAIT cell activation in vivo in parallel with an enhanced therapeutic response. Moreover, TCR-independent activation of MAIT cells leads to a reduction of HCV replication in vitro mediated by IFN-γ. Together these data demonstrate MAIT cells are activated following viral infections, and suggest a potential role in both host defence and immunopathology.",

author = "\{Van Wilgenburg\}, Bonnie and Iris Scherwitzl and Hutchinson, \{Edward C.\} and Tianqi Leng and Ayako Kurioka and Corinna Kulicke and \{De Lara\}, Catherine and Suzanne Cole and Sirijitt Vasanawathana and Wannee Limpitikul and Prida Malasit and Duncan Young and Laura Denney and Moore, \{Michael D.\} and Paolo Fabris and Giordani, \{Maria Teresa\} and Oo, \{Ye Htun\} and Laidlaw, \{Stephen M.\} and Dustin, \{Lynn B.\} and Ho, \{Ling Pei\} and Thompson, \{Fiona M.\} and Narayan Ramamurthy and Juthathip Mongkolsapaya and Willberg, \{Christian B.\} and Screaton, \{Gavin R.\} and Paul Klenerman and Eleanor Barnes and Jonathan Ball and Gary Burgess and Graham Cooke and John Dillon and Charles Gore and Graham Foster and Neil Guha and Rachel Halford and Cham Herath and Chris Holmes and Anita Howe and Emma Hudson and William Irving and Salim Khakoo and Diana Koletzki and Natasha Martin and Tamyo Mbisa and Jane McKeating and John McLauchlan and Alec Miners and Andrea Murray and Peter Shaw and Peter Simmonds",

year = "2016",

month = jun,

day = "23",

doi = "10.1038/ncomms11653",

language = "English",

volume = "7",

journal = "Nature Communications",

issn = "2041-1723",

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Van Wilgenburg, B, Scherwitzl, I, Hutchinson, EC, Leng, T, Kurioka, A, Kulicke, C, De Lara, C, Cole, S, Vasanawathana, S, Limpitikul, W, Malasit, P, Young, D, Denney, L, Moore, MD, Fabris, P, Giordani, MT, Oo, YH, Laidlaw, SM, Dustin, LB, Ho, LP, Thompson, FM, Ramamurthy, N, Mongkolsapaya, J, Willberg, CB, Screaton, GR, Klenerman, P, Barnes, E, Ball, J, Burgess, G, Cooke, G, Dillon, J, Gore, C, Foster, G, Guha, N, Halford, R, Herath, C, Holmes, C, Howe, A, Hudson, E, Irving, W, Khakoo, S, Koletzki, D, Martin, N, Mbisa, T, McKeating, J, McLauchlan, J, Miners, A, Murray, A, Shaw, P & Simmonds, P 2016, 'MAIT cells are activated during human viral infections', Nature Communications, vol. 7, 11653. https://doi.org/10.1038/ncomms11653

TY - JOUR

T1 - MAIT cells are activated during human viral infections

AU - Van Wilgenburg, Bonnie

AU - Scherwitzl, Iris

AU - Hutchinson, Edward C.

AU - Leng, Tianqi

AU - Kurioka, Ayako

AU - Kulicke, Corinna

AU - De Lara, Catherine

AU - Cole, Suzanne

AU - Vasanawathana, Sirijitt

AU - Limpitikul, Wannee

AU - Malasit, Prida

AU - Young, Duncan

AU - Denney, Laura

AU - Moore, Michael D.

AU - Fabris, Paolo

AU - Giordani, Maria Teresa

AU - Oo, Ye Htun

AU - Laidlaw, Stephen M.

AU - Dustin, Lynn B.

AU - Ho, Ling Pei

AU - Thompson, Fiona M.

AU - Ramamurthy, Narayan

AU - Mongkolsapaya, Juthathip

AU - Willberg, Christian B.

AU - Screaton, Gavin R.

AU - Klenerman, Paul

AU - Barnes, Eleanor

AU - Ball, Jonathan

AU - Burgess, Gary

AU - Cooke, Graham

AU - Dillon, John

AU - Gore, Charles

AU - Foster, Graham

AU - Guha, Neil

AU - Halford, Rachel

AU - Herath, Cham

AU - Holmes, Chris

AU - Howe, Anita

AU - Hudson, Emma

AU - Irving, William

AU - Khakoo, Salim

AU - Koletzki, Diana

AU - Martin, Natasha

AU - Mbisa, Tamyo

AU - McKeating, Jane

AU - McLauchlan, John

AU - Miners, Alec

AU - Murray, Andrea

AU - Shaw, Peter

AU - Simmonds, Peter

PY - 2016/6/23

Y1 - 2016/6/23

N2 - Mucosal-associated invariant T (MAIT) cells are abundant in humans and recognize bacterial ligands. Here, we demonstrate that MAIT cells are also activated during human viral infections in vivo. MAIT cells activation was observed during infection with dengue virus, hepatitis C virus and influenza virus. This activation - driving cytokine release and Granzyme B upregulation - is TCR-independent but dependent on IL-18 in synergy with IL-12, IL-15 and/or interferon-α/β. IL-18 levels and MAIT cell activation correlate with disease severity in acute dengue infection. Furthermore, HCV treatment with interferon-α leads to specific MAIT cell activation in vivo in parallel with an enhanced therapeutic response. Moreover, TCR-independent activation of MAIT cells leads to a reduction of HCV replication in vitro mediated by IFN-γ. Together these data demonstrate MAIT cells are activated following viral infections, and suggest a potential role in both host defence and immunopathology.

AB - Mucosal-associated invariant T (MAIT) cells are abundant in humans and recognize bacterial ligands. Here, we demonstrate that MAIT cells are also activated during human viral infections in vivo. MAIT cells activation was observed during infection with dengue virus, hepatitis C virus and influenza virus. This activation - driving cytokine release and Granzyme B upregulation - is TCR-independent but dependent on IL-18 in synergy with IL-12, IL-15 and/or interferon-α/β. IL-18 levels and MAIT cell activation correlate with disease severity in acute dengue infection. Furthermore, HCV treatment with interferon-α leads to specific MAIT cell activation in vivo in parallel with an enhanced therapeutic response. Moreover, TCR-independent activation of MAIT cells leads to a reduction of HCV replication in vitro mediated by IFN-γ. Together these data demonstrate MAIT cells are activated following viral infections, and suggest a potential role in both host defence and immunopathology.

U2 - 10.1038/ncomms11653

DO - 10.1038/ncomms11653

M3 - Article

SN - 2041-1723

VL - 7

JO - Nature Communications

JF - Nature Communications

M1 - 11653

ER -

MAIT cells are activated during human viral infections

Abstract

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