Loop-mediated isothermal amplification test for detection of neisseria gonorrhoeae in urine samples and tolerance of the assay to the presence of urea

Thomas Edwards; Patricia A. Burke; Helen B. Smalley; Liz Gillies; Glyn Hobbs

doi:10.1128/jcm.00314-14

Loop-mediated isothermal amplification test for detection of neisseria gonorrhoeae in urine samples and tolerance of the assay to the presence of urea

Thomas Edwards
, Patricia A. Burke
, Helen B. Smalley
, Liz Gillies
, Glyn Hobbs

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

A loop-mediated isothermal amplification (LAMP) assay for open reading frame 1 (ORF1) of the glutamine synthetase gene of Neisseria gonorrhoeae was able to tolerate urea concentrations of<1.8 M, compared with a PCR assay that was functional at concentrations of<100 mM. The LAMP assay was as sensitive as the PCR assay while being faster and simpler to perform.

Original language	English
Pages (from-to)	2163-2165
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	52
Issue number	6
DOIs	https://doi.org/10.1128/jcm.00314-14
Publication status	Published - 1 Jun 2014
Externally published	Yes

Access to Document

10.1128/jcm.00314-14

Cite this

@article{257c52f2d45e4e798403694776960050,

title = "Loop-mediated isothermal amplification test for detection of neisseria gonorrhoeae in urine samples and tolerance of the assay to the presence of urea",

abstract = "A loop-mediated isothermal amplification (LAMP) assay for open reading frame 1 (ORF1) of the glutamine synthetase gene of Neisseria gonorrhoeae was able to tolerate urea concentrations of<1.8 M, compared with a PCR assay that was functional at concentrations of<100 mM. The LAMP assay was as sensitive as the PCR assay while being faster and simpler to perform.",

author = "Thomas Edwards and Burke, \{Patricia A.\} and Smalley, \{Helen B.\} and Liz Gillies and Glyn Hobbs",

year = "2014",

month = jun,

day = "1",

doi = "10.1128/jcm.00314-14",

language = "English",

volume = "52",

pages = "2163--2165",

journal = "Journal of Clinical Microbiology",

issn = "0095-1137",

publisher = "American Society for Microbiology",

number = "6",

}

TY - JOUR

T1 - Loop-mediated isothermal amplification test for detection of neisseria gonorrhoeae in urine samples and tolerance of the assay to the presence of urea

AU - Edwards, Thomas

AU - Burke, Patricia A.

AU - Smalley, Helen B.

AU - Gillies, Liz

AU - Hobbs, Glyn

PY - 2014/6/1

Y1 - 2014/6/1

N2 - A loop-mediated isothermal amplification (LAMP) assay for open reading frame 1 (ORF1) of the glutamine synthetase gene of Neisseria gonorrhoeae was able to tolerate urea concentrations of<1.8 M, compared with a PCR assay that was functional at concentrations of<100 mM. The LAMP assay was as sensitive as the PCR assay while being faster and simpler to perform.

AB - A loop-mediated isothermal amplification (LAMP) assay for open reading frame 1 (ORF1) of the glutamine synthetase gene of Neisseria gonorrhoeae was able to tolerate urea concentrations of<1.8 M, compared with a PCR assay that was functional at concentrations of<100 mM. The LAMP assay was as sensitive as the PCR assay while being faster and simpler to perform.

U2 - 10.1128/jcm.00314-14

DO - 10.1128/jcm.00314-14

M3 - Article

SN - 0095-1137

VL - 52

SP - 2163

EP - 2165

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 6

ER -

Loop-mediated isothermal amplification test for detection of neisseria gonorrhoeae in urine samples and tolerance of the assay to the presence of urea

Abstract

Access to Document

Fingerprint

Cite this