Long chain poly-unsaturated fatty acids attenuate the IL-1β-induced proinflammatory response in human fetal intestinal epithelial cells

Vasuki Wijendran; J.T. Brenna; Dong Hao Wang; Weishu Zhu; Di Meng; Kriston Ganguli; Kumar S.D. Kothapalli; Pilar Requena; Sheila Innis; W. Allan Walker

doi:10.1038/pr.2015.154

Long chain poly-unsaturated fatty acids attenuate the IL-1β-induced proinflammatory response in human fetal intestinal epithelial cells

Vasuki Wijendran, J.T. Brenna, Dong Hao Wang, Weishu Zhu, Di Meng, Kriston Ganguli, Kumar S.D. Kothapalli, Pilar Requena, Sheila Innis, W. Allan Walker

Tropical Disease Biology

Liverpool School of Tropical Medicine

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

Background

Evidence suggests that excessive inflammation of the immature intestine may predispose premature infants to necrotizing enterocolitis (NEC). We investigated the anti-inflammatory effects of docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), and arachidonic acid (ARA) in human fetal and adult intestinal epithelial cells (IEC) in primary culture.

Methods

Human fetal IEC in culture were derived from a healthy fetal small intestine (H4) or resected small intestine of a neonate with NEC (NEC-IEC). Intestinal cell lines Caco2 and NCM460 in culture were used as models for mature IEC. IEC in culture were pretreated with 100 µmol/l palmitic acid (PAL), DHA, EPA, ARA, or ARA+DHA for 48 h and then stimulated with proinflammatory IL-1β

Results.

DHA significantly attenuated IL-1β induced proinflammatory IL-8 and IL-6 protein and mRNA in fetal H4, NEC-IEC, and mature Caco2, NCM460 IEC, compared to control and PAL treatment. DHA downregulated IL-1R1 (IL-1β receptor) and NFk β1 mRNA expression in fetal and adult IEC. ARA had potent anti-inflammatory effects with lower IL-8 and IL-6 (protein and mRNA) in fetal H4 but not in NEC-IEC or adult IEC.

Conclusion

The present study provides evidence that DHA and ARA may have important anti-inflammatory functions for prevention of NEC in premature infants.

Original language	English
Pages (from-to)	626-633
Number of pages	8
Journal	Pediatric Research
Volume	78
Issue number	6
DOIs	https://doi.org/10.1038/pr.2015.154
Publication status	Published - 23 Sept 2015

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title = "Long chain poly-unsaturated fatty acids attenuate the IL-1β-induced proinflammatory response in human fetal intestinal epithelial cells",

abstract = "BackgroundEvidence suggests that excessive inflammation of the immature intestine may predispose premature infants to necrotizing enterocolitis (NEC). We investigated the anti-inflammatory effects of docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), and arachidonic acid (ARA) in human fetal and adult intestinal epithelial cells (IEC) in primary culture.MethodsHuman fetal IEC in culture were derived from a healthy fetal small intestine (H4) or resected small intestine of a neonate with NEC (NEC-IEC). Intestinal cell lines Caco2 and NCM460 in culture were used as models for mature IEC. IEC in culture were pretreated with 100 µmol/l palmitic acid (PAL), DHA, EPA, ARA, or ARA+DHA for 48 h and then stimulated with proinflammatory IL-1βResults.DHA significantly attenuated IL-1β induced proinflammatory IL-8 and IL-6 protein and mRNA in fetal H4, NEC-IEC, and mature Caco2, NCM460 IEC, compared to control and PAL treatment. DHA downregulated IL-1R1 (IL-1β receptor) and NFk β1 mRNA expression in fetal and adult IEC. ARA had potent anti-inflammatory effects with lower IL-8 and IL-6 (protein and mRNA) in fetal H4 but not in NEC-IEC or adult IEC.ConclusionThe present study provides evidence that DHA and ARA may have important anti-inflammatory functions for prevention of NEC in premature infants.",

author = "Vasuki Wijendran and J.T. Brenna and Wang, \{Dong Hao\} and Weishu Zhu and Di Meng and Kriston Ganguli and Kothapalli, \{Kumar S.D.\} and Pilar Requena and Sheila Innis and Walker, \{W. Allan\}",

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doi = "10.1038/pr.2015.154",

language = "English",

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T1 - Long chain poly-unsaturated fatty acids attenuate the IL-1β-induced proinflammatory response in human fetal intestinal epithelial cells

AU - Wijendran, Vasuki

AU - Brenna, J.T.

AU - Wang, Dong Hao

AU - Zhu, Weishu

AU - Meng, Di

AU - Ganguli, Kriston

AU - Kothapalli, Kumar S.D.

AU - Requena, Pilar

AU - Innis, Sheila

AU - Walker, W. Allan

PY - 2015/9/23

Y1 - 2015/9/23

N2 - BackgroundEvidence suggests that excessive inflammation of the immature intestine may predispose premature infants to necrotizing enterocolitis (NEC). We investigated the anti-inflammatory effects of docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), and arachidonic acid (ARA) in human fetal and adult intestinal epithelial cells (IEC) in primary culture.MethodsHuman fetal IEC in culture were derived from a healthy fetal small intestine (H4) or resected small intestine of a neonate with NEC (NEC-IEC). Intestinal cell lines Caco2 and NCM460 in culture were used as models for mature IEC. IEC in culture were pretreated with 100 µmol/l palmitic acid (PAL), DHA, EPA, ARA, or ARA+DHA for 48 h and then stimulated with proinflammatory IL-1βResults.DHA significantly attenuated IL-1β induced proinflammatory IL-8 and IL-6 protein and mRNA in fetal H4, NEC-IEC, and mature Caco2, NCM460 IEC, compared to control and PAL treatment. DHA downregulated IL-1R1 (IL-1β receptor) and NFk β1 mRNA expression in fetal and adult IEC. ARA had potent anti-inflammatory effects with lower IL-8 and IL-6 (protein and mRNA) in fetal H4 but not in NEC-IEC or adult IEC.ConclusionThe present study provides evidence that DHA and ARA may have important anti-inflammatory functions for prevention of NEC in premature infants.

AB - BackgroundEvidence suggests that excessive inflammation of the immature intestine may predispose premature infants to necrotizing enterocolitis (NEC). We investigated the anti-inflammatory effects of docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), and arachidonic acid (ARA) in human fetal and adult intestinal epithelial cells (IEC) in primary culture.MethodsHuman fetal IEC in culture were derived from a healthy fetal small intestine (H4) or resected small intestine of a neonate with NEC (NEC-IEC). Intestinal cell lines Caco2 and NCM460 in culture were used as models for mature IEC. IEC in culture were pretreated with 100 µmol/l palmitic acid (PAL), DHA, EPA, ARA, or ARA+DHA for 48 h and then stimulated with proinflammatory IL-1βResults.DHA significantly attenuated IL-1β induced proinflammatory IL-8 and IL-6 protein and mRNA in fetal H4, NEC-IEC, and mature Caco2, NCM460 IEC, compared to control and PAL treatment. DHA downregulated IL-1R1 (IL-1β receptor) and NFk β1 mRNA expression in fetal and adult IEC. ARA had potent anti-inflammatory effects with lower IL-8 and IL-6 (protein and mRNA) in fetal H4 but not in NEC-IEC or adult IEC.ConclusionThe present study provides evidence that DHA and ARA may have important anti-inflammatory functions for prevention of NEC in premature infants.

U2 - 10.1038/pr.2015.154

DO - 10.1038/pr.2015.154

M3 - Article

SN - 0031-3998

VL - 78

SP - 626

EP - 633

JO - Pediatric Research

JF - Pediatric Research

IS - 6

ER -

Long chain poly-unsaturated fatty acids attenuate the IL-1β-induced proinflammatory response in human fetal intestinal epithelial cells

Abstract

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