Linkage of a novel mercury resistance operon with streptomycin resistance on a conjugative plasmid in Enterococcus faecium

Ian J. Davis; Adam Roberts; Derren Ready; Hilary Richards; Michael Wilson; Peter Mullany

doi:10.1016/j.plasmid.2004.10.004

Linkage of a novel mercury resistance operon with streptomycin resistance on a conjugative plasmid in Enterococcus faecium

Ian J. Davis, Adam Roberts, Derren Ready, Hilary Richards, Michael Wilson, Peter Mullany

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

It has been shown that the mercury in dental amalgam and other environmental sources can select for mercury resistant bacteria and that this can lead to an increase in resistance to antibiotics. To understand more about this linkage we have investigated the genetic basis for mercury and antibiotic resistance in a variety of oral bacteria. In this study we have cloned and sequenced the mer operon from an Enterococcus faecium strain which was resistant to mercury, tetracycline, and streptomycin. This strain was isolated, in a previous investigation, from a cynomolgus monkey post-installation of amalgam fillings. The mer operon was contained within a putative transposon (Tnmer1) of the ISL3 family. This element was located on a streptomycin resistant plasmid, pPPM1000, which shares homology with pRE25.

Original language	English
Pages (from-to)	26-38
Number of pages	13
Journal	Plasmid
Volume	54
Issue number	1
DOIs	https://doi.org/10.1016/j.plasmid.2004.10.004
Publication status	Published - 1 Jul 2005
Externally published	Yes

Keywords

Enterococcus faecium
ISL3
Mercury resistance
pRE25

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10.1016/j.plasmid.2004.10.004

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title = "Linkage of a novel mercury resistance operon with streptomycin resistance on a conjugative plasmid in Enterococcus faecium",

abstract = "It has been shown that the mercury in dental amalgam and other environmental sources can select for mercury resistant bacteria and that this can lead to an increase in resistance to antibiotics. To understand more about this linkage we have investigated the genetic basis for mercury and antibiotic resistance in a variety of oral bacteria. In this study we have cloned and sequenced the mer operon from an Enterococcus faecium strain which was resistant to mercury, tetracycline, and streptomycin. This strain was isolated, in a previous investigation, from a cynomolgus monkey post-installation of amalgam fillings. The mer operon was contained within a putative transposon (Tnmer1) of the ISL3 family. This element was located on a streptomycin resistant plasmid, pPPM1000, which shares homology with pRE25.",

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T1 - Linkage of a novel mercury resistance operon with streptomycin resistance on a conjugative plasmid in Enterococcus faecium

AU - Davis, Ian J.

AU - Roberts, Adam

AU - Ready, Derren

AU - Richards, Hilary

AU - Wilson, Michael

AU - Mullany, Peter

PY - 2005/7/1

Y1 - 2005/7/1

N2 - It has been shown that the mercury in dental amalgam and other environmental sources can select for mercury resistant bacteria and that this can lead to an increase in resistance to antibiotics. To understand more about this linkage we have investigated the genetic basis for mercury and antibiotic resistance in a variety of oral bacteria. In this study we have cloned and sequenced the mer operon from an Enterococcus faecium strain which was resistant to mercury, tetracycline, and streptomycin. This strain was isolated, in a previous investigation, from a cynomolgus monkey post-installation of amalgam fillings. The mer operon was contained within a putative transposon (Tnmer1) of the ISL3 family. This element was located on a streptomycin resistant plasmid, pPPM1000, which shares homology with pRE25.

AB - It has been shown that the mercury in dental amalgam and other environmental sources can select for mercury resistant bacteria and that this can lead to an increase in resistance to antibiotics. To understand more about this linkage we have investigated the genetic basis for mercury and antibiotic resistance in a variety of oral bacteria. In this study we have cloned and sequenced the mer operon from an Enterococcus faecium strain which was resistant to mercury, tetracycline, and streptomycin. This strain was isolated, in a previous investigation, from a cynomolgus monkey post-installation of amalgam fillings. The mer operon was contained within a putative transposon (Tnmer1) of the ISL3 family. This element was located on a streptomycin resistant plasmid, pPPM1000, which shares homology with pRE25.

KW - Enterococcus faecium

KW - ISL3

KW - Mercury resistance

KW - pRE25

U2 - 10.1016/j.plasmid.2004.10.004

DO - 10.1016/j.plasmid.2004.10.004

M3 - Article

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VL - 54

SP - 26

EP - 38

JO - Plasmid

JF - Plasmid

IS - 1

ER -

Linkage of a novel mercury resistance operon with streptomycin resistance on a conjugative plasmid in Enterococcus faecium

Abstract

Keywords

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