Leishmania donovani complex: Genotyping with the ribosomal internal transcribed spacer and the mini-exon: Genotyping with the ribosomal internal transcribed spacer and the mini-exon

I. L. Mauricio; Russell Stothard; M. A. Miles

doi:10.1017/s0031182003004578

Leishmania donovani complex: Genotyping with the ribosomal internal transcribed spacer and the mini-exon: Genotyping with the ribosomal internal transcribed spacer and the mini-exon

I. L. Mauricio, Russell Stothard, M. A. Miles

Research output: Contribution to journal › Article › peer-review

69 Citations (Scopus)

Abstract

Intergenic region typing by restriction analysis of the ribosomal internal transcribed spacer (ITS) and mini-exon provide diagnostic markers for some Leishmania. Here, we evaluate restriction analysis of these targets for genotyping and phylogenetic analysis within the Leishmania donovani complex (agents of visceral leishmaniasis). Each method was useful for genotyping of both L. donovani complex strains and Old World Leishmania species. The targets produced less robust groups than gp63 intergenic regions, but support the need for re-evaluation of the taxonomy of the L. donovani complex.

Original language	English
Pages (from-to)	263-267
Number of pages	5
Journal	Parasitology
Volume	128
Issue number	3
DOIs	https://doi.org/10.1017/s0031182003004578
Publication status	Published - 1 Mar 2004
Externally published	Yes

Keywords

Genotyping
Kinetoplastida
Leishmania donovani
Mini-exon
Restriction fragment length polymorphism
Ribosomal internal transcribed spacer

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10.1017/s0031182003004578

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title = "Leishmania donovani complex: Genotyping with the ribosomal internal transcribed spacer and the mini-exon: Genotyping with the ribosomal internal transcribed spacer and the mini-exon",

abstract = "Intergenic region typing by restriction analysis of the ribosomal internal transcribed spacer (ITS) and mini-exon provide diagnostic markers for some Leishmania. Here, we evaluate restriction analysis of these targets for genotyping and phylogenetic analysis within the Leishmania donovani complex (agents of visceral leishmaniasis). Each method was useful for genotyping of both L. donovani complex strains and Old World Leishmania species. The targets produced less robust groups than gp63 intergenic regions, but support the need for re-evaluation of the taxonomy of the L. donovani complex.",

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T1 - Leishmania donovani complex: Genotyping with the ribosomal internal transcribed spacer and the mini-exon: Genotyping with the ribosomal internal transcribed spacer and the mini-exon

AU - Mauricio, I. L.

AU - Stothard, Russell

AU - Miles, M. A.

PY - 2004/3/1

Y1 - 2004/3/1

N2 - Intergenic region typing by restriction analysis of the ribosomal internal transcribed spacer (ITS) and mini-exon provide diagnostic markers for some Leishmania. Here, we evaluate restriction analysis of these targets for genotyping and phylogenetic analysis within the Leishmania donovani complex (agents of visceral leishmaniasis). Each method was useful for genotyping of both L. donovani complex strains and Old World Leishmania species. The targets produced less robust groups than gp63 intergenic regions, but support the need for re-evaluation of the taxonomy of the L. donovani complex.

AB - Intergenic region typing by restriction analysis of the ribosomal internal transcribed spacer (ITS) and mini-exon provide diagnostic markers for some Leishmania. Here, we evaluate restriction analysis of these targets for genotyping and phylogenetic analysis within the Leishmania donovani complex (agents of visceral leishmaniasis). Each method was useful for genotyping of both L. donovani complex strains and Old World Leishmania species. The targets produced less robust groups than gp63 intergenic regions, but support the need for re-evaluation of the taxonomy of the L. donovani complex.

KW - Genotyping

KW - Kinetoplastida

KW - Leishmania donovani

KW - Mini-exon

KW - Restriction fragment length polymorphism

KW - Ribosomal internal transcribed spacer

U2 - 10.1017/s0031182003004578

DO - 10.1017/s0031182003004578

M3 - Article

SN - 0031-1820

VL - 128

SP - 263

EP - 267

JO - Parasitology

JF - Parasitology

IS - 3

ER -

Leishmania donovani complex: Genotyping with the ribosomal internal transcribed spacer and the mini-exon: Genotyping with the ribosomal internal transcribed spacer and the mini-exon

Abstract

Keywords

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