Integrated de novo transcriptome of Culex pipiens mosquito larvae as a resource for genetic control strategies

Valentina Mastrantonio; Pietro Libro; Jessica Di Martino; Michele Matera; Romeo Bellini; Tiziana Castrignanò; Sandra Urbanelli; Daniele Porretta

doi:10.1038/s41597-024-03285-1

Integrated de novo transcriptome of Culex pipiens mosquito larvae as a resource for genetic control strategies

Valentina Mastrantonio, Pietro Libro, Jessica Di Martino, Michele Matera, Romeo Bellini, Tiziana Castrignanò, Sandra Urbanelli, Daniele Porretta

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

We present a de novo transcriptome of the mosquito vector Culex pipiens, assembled by sequences of susceptible and insecticide resistant larvae. The high quality of the assembly was confirmed by TransRate and BUSCO. A mapping percentage until 94.8% was obtained by aligning contigs to Nr, SwissProt, and TrEMBL, with 27,281 sequences that simultaneously mapped on the three databases. A total of 14,966 ORFs were also functionally annotated by using the eggNOG database. Among them, we identified ORF sequences of the main gene families involved in insecticide resistance. Therefore, this resource stands as a valuable reference for further studies of differential gene expression as well as to identify genes of interest for genetic-based control tools.

Original language	English
Article number	471
Pages (from-to)	e471
Journal	Scientific data
Volume	11
Issue number	1
Early online date	9 May 2024
DOIs	https://doi.org/10.1038/s41597-024-03285-1
Publication status	Published - 9 May 2024

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41597 2024 Article 3285Final published version, 1.57 MBLicence: CC BY

Data for 'Integrated de novo transcriptome of Culex pipiens mosquito larvae as a resource for genetic control strategies'
Matera, M. (Creator), tbc, 16 May 2024
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title = "Integrated de novo transcriptome of Culex pipiens mosquito larvae as a resource for genetic control strategies",

abstract = "We present a de novo transcriptome of the mosquito vector Culex pipiens, assembled by sequences of susceptible and insecticide resistant larvae. The high quality of the assembly was confirmed by TransRate and BUSCO. A mapping percentage until 94.8\% was obtained by aligning contigs to Nr, SwissProt, and TrEMBL, with 27,281 sequences that simultaneously mapped on the three databases. A total of 14,966 ORFs were also functionally annotated by using the eggNOG database. Among them, we identified ORF sequences of the main gene families involved in insecticide resistance. Therefore, this resource stands as a valuable reference for further studies of differential gene expression as well as to identify genes of interest for genetic-based control tools.",

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T1 - Integrated de novo transcriptome of Culex pipiens mosquito larvae as a resource for genetic control strategies

AU - Mastrantonio, Valentina

AU - Libro, Pietro

AU - Di Martino, Jessica

AU - Matera, Michele

AU - Bellini, Romeo

AU - Castrignanò, Tiziana

AU - Urbanelli, Sandra

AU - Porretta, Daniele

PY - 2024/5/9

Y1 - 2024/5/9

N2 - We present a de novo transcriptome of the mosquito vector Culex pipiens, assembled by sequences of susceptible and insecticide resistant larvae. The high quality of the assembly was confirmed by TransRate and BUSCO. A mapping percentage until 94.8% was obtained by aligning contigs to Nr, SwissProt, and TrEMBL, with 27,281 sequences that simultaneously mapped on the three databases. A total of 14,966 ORFs were also functionally annotated by using the eggNOG database. Among them, we identified ORF sequences of the main gene families involved in insecticide resistance. Therefore, this resource stands as a valuable reference for further studies of differential gene expression as well as to identify genes of interest for genetic-based control tools.

AB - We present a de novo transcriptome of the mosquito vector Culex pipiens, assembled by sequences of susceptible and insecticide resistant larvae. The high quality of the assembly was confirmed by TransRate and BUSCO. A mapping percentage until 94.8% was obtained by aligning contigs to Nr, SwissProt, and TrEMBL, with 27,281 sequences that simultaneously mapped on the three databases. A total of 14,966 ORFs were also functionally annotated by using the eggNOG database. Among them, we identified ORF sequences of the main gene families involved in insecticide resistance. Therefore, this resource stands as a valuable reference for further studies of differential gene expression as well as to identify genes of interest for genetic-based control tools.

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DO - 10.1038/s41597-024-03285-1

M3 - Article

SN - 2052-4463

VL - 11

SP - e471

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JF - Scientific data

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ER -

Integrated de novo transcriptome of Culex pipiens mosquito larvae as a resource for genetic control strategies

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