In vitro biomarker discovery in the parasitic flatworm Fasciola hepatica for monitoring chemotherapeutic treatment

Russell M. Morphew; Neil MacKintosh; Elizabeth H. Hart; Mark Prescott; James LaCourse; Peter M. Brophy

doi:10.1016/j.euprot.2014.02.013

In vitro biomarker discovery in the parasitic flatworm Fasciola hepatica for monitoring chemotherapeutic treatment

Russell M. Morphew
, Neil MacKintosh
, Elizabeth H. Hart
, Mark Prescott
, James LaCourse
, Peter M. Brophy

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

The parasitic flatworm Fasciola hepatica is a global food security risk. With no vaccines, the sustainability of triclabendazole (TCBZ) is threatened by emerging resistance. F. hepatica excretory/secretory (ES) products can be detected in host faeces and used to estimate TCBZ success and failure. However, there are no faecal based molecular diagnostics dedicated to assessing drug failure or resistance to TCBZ in the field. Utilising in vitro maintenance and sub-proteomic approaches two TCBZ stress ES protein response fingerprints were identified: markers of non-killing and lethal doses. This study provides candidate protein/peptide biomarkers to validate for detection of TCBZ failure and resistance.

Original language	English
Pages (from-to)	85-99
Number of pages	15
Journal	EuPA Open Proteomics
Volume	3
DOIs	https://doi.org/10.1016/j.euprot.2014.02.013
Publication status	Published - 1 Jun 2014

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 2 Zero Hunger
SDG 3 Good Health and Well-being

Keywords

Biomarkers
Calreticulin
Proteomics
Triclabendazole

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10.1016/j.euprot.2014.02.013Licence: CC BY-NC-SA

1-s2Final published version, 1.43 MBLicence: CC BY-NC

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title = "In vitro biomarker discovery in the parasitic flatworm Fasciola hepatica for monitoring chemotherapeutic treatment",

abstract = "The parasitic flatworm Fasciola hepatica is a global food security risk. With no vaccines, the sustainability of triclabendazole (TCBZ) is threatened by emerging resistance. F. hepatica excretory/secretory (ES) products can be detected in host faeces and used to estimate TCBZ success and failure. However, there are no faecal based molecular diagnostics dedicated to assessing drug failure or resistance to TCBZ in the field. Utilising in vitro maintenance and sub-proteomic approaches two TCBZ stress ES protein response fingerprints were identified: markers of non-killing and lethal doses. This study provides candidate protein/peptide biomarkers to validate for detection of TCBZ failure and resistance.",

keywords = "Biomarkers, Calreticulin, Proteomics, Triclabendazole",

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doi = "10.1016/j.euprot.2014.02.013",

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T1 - In vitro biomarker discovery in the parasitic flatworm Fasciola hepatica for monitoring chemotherapeutic treatment

AU - Morphew, Russell M.

AU - MacKintosh, Neil

AU - Hart, Elizabeth H.

AU - Prescott, Mark

AU - LaCourse, James

AU - Brophy, Peter M.

PY - 2014/6/1

Y1 - 2014/6/1

N2 - The parasitic flatworm Fasciola hepatica is a global food security risk. With no vaccines, the sustainability of triclabendazole (TCBZ) is threatened by emerging resistance. F. hepatica excretory/secretory (ES) products can be detected in host faeces and used to estimate TCBZ success and failure. However, there are no faecal based molecular diagnostics dedicated to assessing drug failure or resistance to TCBZ in the field. Utilising in vitro maintenance and sub-proteomic approaches two TCBZ stress ES protein response fingerprints were identified: markers of non-killing and lethal doses. This study provides candidate protein/peptide biomarkers to validate for detection of TCBZ failure and resistance.

AB - The parasitic flatworm Fasciola hepatica is a global food security risk. With no vaccines, the sustainability of triclabendazole (TCBZ) is threatened by emerging resistance. F. hepatica excretory/secretory (ES) products can be detected in host faeces and used to estimate TCBZ success and failure. However, there are no faecal based molecular diagnostics dedicated to assessing drug failure or resistance to TCBZ in the field. Utilising in vitro maintenance and sub-proteomic approaches two TCBZ stress ES protein response fingerprints were identified: markers of non-killing and lethal doses. This study provides candidate protein/peptide biomarkers to validate for detection of TCBZ failure and resistance.

KW - Biomarkers

KW - Calreticulin

KW - Proteomics

KW - Triclabendazole

U2 - 10.1016/j.euprot.2014.02.013

DO - 10.1016/j.euprot.2014.02.013

M3 - Article

SN - 2212-9685

VL - 3

SP - 85

EP - 99

JO - EuPA Open Proteomics

JF - EuPA Open Proteomics

ER -

In vitro biomarker discovery in the parasitic flatworm Fasciola hepatica for monitoring chemotherapeutic treatment

Abstract

UN SDGs

Keywords

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