Improved three-dimensional localization of multiple small objects in close proximity in digital holography

Matthew L. Hall; Philip McCall; Catherine E. Towers; David P. Towers

doi:10.1364/ao.404432

Improved three-dimensional localization of multiple small objects in close proximity in digital holography

Matthew L. Hall, Philip McCall, Catherine E. Towers, David P. Towers

Vector Biology

University of Warwick

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Using intensity gradient- or sparsity-based focus metrics, the ability to accurately localize the three-dimensional (3D) position of a small object in a digital holographic reconstruction of a large field of view is hindered in the presence of multiple nearby objects. A more accurate alternative method for 3D localization, based on evaluation of the complex reconstructed volume, is proposed. Simulations and experimental data demonstrate a reduction in depth positional error for single objects and a notably improved axial resolution of multiple objects in close proximity.

Original language	English
Pages (from-to)	A285-A295
Journal	Applied Optics
Volume	60
Issue number	4
Early online date	4 Dec 2020
DOIs	https://doi.org/10.1364/ao.404432
Publication status	Published - 1 Feb 2021

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10.1364/ao.404432Licence: CC BY

Hall et al App Optics 2020Final published version, 3.01 MBLicence: CC BY

Cite this

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title = "Improved three-dimensional localization of multiple small objects in close proximity in digital holography",

abstract = "Using intensity gradient- or sparsity-based focus metrics, the ability to accurately localize the three-dimensional (3D) position of a small object in a digital holographic reconstruction of a large field of view is hindered in the presence of multiple nearby objects. A more accurate alternative method for 3D localization, based on evaluation of the complex reconstructed volume, is proposed. Simulations and experimental data demonstrate a reduction in depth positional error for single objects and a notably improved axial resolution of multiple objects in close proximity.",

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AB - Using intensity gradient- or sparsity-based focus metrics, the ability to accurately localize the three-dimensional (3D) position of a small object in a digital holographic reconstruction of a large field of view is hindered in the presence of multiple nearby objects. A more accurate alternative method for 3D localization, based on evaluation of the complex reconstructed volume, is proposed. Simulations and experimental data demonstrate a reduction in depth positional error for single objects and a notably improved axial resolution of multiple objects in close proximity.

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JO - Applied Optics

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Improved three-dimensional localization of multiple small objects in close proximity in digital holography

Abstract

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