Identifying vulnerable pathways in Mycobacterium tuberculosis by using a knockdown approach

Paul Carroll; Marie Claire Faray-Kele; Tanya Parish

doi:10.1128/AEM.02880-10

Identifying vulnerable pathways in Mycobacterium tuberculosis by using a knockdown approach

Paul Carroll
, Marie Claire Faray-Kele
, Tanya Parish

Queen Mary University of London
Infectious Disease Research Institute

Research output: Contribution to journal › Article › peer-review

69 Citations (Scopus)

Abstract

We constructed recombinant strains of Mycobacterium tuberculosis in which expression of specific genes was downregulated to identify vulnerable drug targets. Growth phenotypes in macrophages and culture were used to rank targets: the dprE1, clpP1, and fadD32 operons were the best targets and glnA1, glnE, pknL, regX3, and senX3 were poor targets.

Original language	English
Pages (from-to)	5040-5043
Number of pages	4
Journal	Applied and Environmental Microbiology
Volume	77
Issue number	14
DOIs	https://doi.org/10.1128/AEM.02880-10
Publication status	Published - 7 Jul 2011
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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10.1128/AEM.02880-10

Cite this

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title = "Identifying vulnerable pathways in Mycobacterium tuberculosis by using a knockdown approach",

abstract = "We constructed recombinant strains of Mycobacterium tuberculosis in which expression of specific genes was downregulated to identify vulnerable drug targets. Growth phenotypes in macrophages and culture were used to rank targets: the dprE1, clpP1, and fadD32 operons were the best targets and glnA1, glnE, pknL, regX3, and senX3 were poor targets.",

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AB - We constructed recombinant strains of Mycobacterium tuberculosis in which expression of specific genes was downregulated to identify vulnerable drug targets. Growth phenotypes in macrophages and culture were used to rank targets: the dprE1, clpP1, and fadD32 operons were the best targets and glnA1, glnE, pknL, regX3, and senX3 were poor targets.

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JO - Applied and Environmental Microbiology

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Identifying vulnerable pathways in Mycobacterium tuberculosis by using a knockdown approach

Abstract

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