TY - JOUR
T1 - Genome-wide association studies reveal novel loci associated with pyrethroid and organophosphate resistance in Anopheles gambiae and Anopheles coluzzii
AU - Lucas, Eric
AU - Nagi, Sanjay
AU - Egyir-Yawson, Alexander
AU - Essandoh, John
AU - Dadzie, Samuel
AU - Chabi, Joseph
AU - Djogbénou, Luc S.
AU - Medjigbodo, Adandé A.
AU - Edi, Constant V.
AU - Kétoh, Guillaume K.
AU - Koudou, Benjamin G.
AU - Van’t Hof, Arjen E.
AU - Rippon, Emily
AU - Pipini, Dimitra
AU - Harding, Nicholas J.
AU - Dyer, Naomi
AU - Cerdeira, Louise
AU - Clarkson, Chris S.
AU - Kwiatkowski, Dominic P.
AU - Miles, Alistair
AU - Donnelly, Martin
AU - Weetman, David
PY - 2023/8/16
Y1 - 2023/8/16
N2 - Resistance to insecticides in Anopheles mosquitoes threatens the effectiveness of malaria control, but the genetics of resistance are only partially understood. We performed a large scale multi-country genome-wide association study of resistance to two widely used insecticides: deltamethrin and pirimiphos-methyl, using sequencing data from An. gambiae and An. coluzzii from ten locations in West Africa. Resistance was highly multi-genic, multiallelic and variable between populations. While the strongest and most consistent association with deltamethrin resistance came from Cyp6aa1, this was based on several independent copy number variants (CNVs) in An. coluzzii, and on a non-CNV haplotype in An. gambiae. For pirimiphos-methyl, signals included Ace1, cytochrome P450s, glutathione S-transferases and the nAChR target site of neonicotinoid insecticides. The regions around Cyp9k1 and the Tep family of immune genes showed evidence of cross-resistance to bothinsecticides. These locally-varying, multi-allelic patterns highlight the challenges involved in genomic monitoring of resistance, and form the basis for improved surveillance methods.
AB - Resistance to insecticides in Anopheles mosquitoes threatens the effectiveness of malaria control, but the genetics of resistance are only partially understood. We performed a large scale multi-country genome-wide association study of resistance to two widely used insecticides: deltamethrin and pirimiphos-methyl, using sequencing data from An. gambiae and An. coluzzii from ten locations in West Africa. Resistance was highly multi-genic, multiallelic and variable between populations. While the strongest and most consistent association with deltamethrin resistance came from Cyp6aa1, this was based on several independent copy number variants (CNVs) in An. coluzzii, and on a non-CNV haplotype in An. gambiae. For pirimiphos-methyl, signals included Ace1, cytochrome P450s, glutathione S-transferases and the nAChR target site of neonicotinoid insecticides. The regions around Cyp9k1 and the Tep family of immune genes showed evidence of cross-resistance to bothinsecticides. These locally-varying, multi-allelic patterns highlight the challenges involved in genomic monitoring of resistance, and form the basis for improved surveillance methods.
U2 - 10.1038/s41467-023-40693-0
DO - 10.1038/s41467-023-40693-0
M3 - Article
SN - 2041-1723
VL - 14
SP - e4946
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 4946
ER -
