Genetic structure and insecticide resistance characteristics of fall armyworm populations invading China

Lei Zhang; Bo Liu; Weigang Zheng; Conghui Liu; Dandan Zhang; Shengyuan Zhao; Zaiyuan Li; Pengjun Xu; Kenneth Wilson; Amy Withers; Chris Jones; Judith A. Smith; Gilson Chipabika; Donald L. Kachigamba; Kiwoong Nam; Emmanuelle d’Alençon; Bei Liu; Xinyue Liang; Minghui Jin; Chao Wu; Swapan Chakrabarty; Xianming Yang; Yuying Jiang; Jie Liu; Xiaolin Liu; Weipeng Quan; Guirong Wang; Wei Fan; Wanqiang Qian; Kongming Wu; Yutao Xiao

doi:10.1111/1755-0998.13219

Genetic structure and insecticide resistance characteristics of fall armyworm populations invading China

Lei Zhang, Bo Liu, Weigang Zheng, Conghui Liu, Dandan Zhang, Shengyuan Zhao, Zaiyuan Li, Pengjun Xu, Kenneth Wilson, Amy Withers, Chris Jones, Judith A. Smith, Gilson Chipabika, Donald L. Kachigamba, Kiwoong Nam, Emmanuelle d’Alençon, Bei Liu, Xinyue Liang, Minghui Jin, Chao WuSwapan Chakrabarty, Xianming Yang, Yuying Jiang, Jie Liu, Xiaolin Liu, Weipeng Quan, Guirong Wang, Wei Fan, Wanqiang Qian, Kongming Wu, Yutao Xiao

Research output: Contribution to journal › Article › peer-review

164 Citations (Scopus)

Abstract

The rapid wide‐scale spread of fall armyworm (Spodoptera frugiperda ) has caused serious crop losses globally. However, differences in the genetic background of subpopulations and the mechanisms of rapid adaptation behind the invasion are still not well understood. Here we report the assembly of a 390.38Mb chromosome‐level genome of fall armyworm derived from south‐central Africa using Pacific Bioscience (PacBio) and Hi‐C sequencing technologies, with scaffold N50 of 12.9 Mb and containing 22260 annotated protein‐coding genes. Genome‐wide resequencing of 103 samples and strain identification were conducted to reveal the genetic background of fall armyworm populations in China. Analysis of genes related to pesticide‐ and Bt‐resistance showed that the risk of fall armyworm developing resistance to conventional pesticides is very high. Laboratory bioassay results showed that insects invading China carry resistance to organophosphate and pyrethroid pesticides, but are sensitive to genetically modified maize expressing the Bacillus thuringiensis (Bt) toxin Cry1Ab in field experiments. Additionally, two mitochondrial fragments were found to be inserted into the nuclear genome, with the insertion event occurring after the differentiation of the two strains. This study represents a valuable advance toward improving management strategies for fall armyworm.

Original language	English
Pages (from-to)	1682-1696
Number of pages	15
Journal	Molecular Ecology Resources
Volume	20
Issue number	6
Early online date	3 Jul 2020
DOIs	https://doi.org/10.1111/1755-0998.13219
Publication status	Published - 1 Nov 2020
Externally published	Yes

Keywords

gene insertion
population structure
resistance risk
Spodoptera frugiperda
subpopulations

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10.1111/1755-0998.13219Licence: CC BY

2020 ZHANG MOL ECOL RES ACCEPTEDAccepted author manuscript, 13.4 MB

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Zhang, L., Liu, B., Zheng, W., Liu, C., Zhang, D., Zhao, S., Li, Z., Xu, P., Wilson, K., Withers, A., Jones, C., Smith, J. A., Chipabika, G., Kachigamba, D. L., Nam, K., d’Alençon, E., Liu, B., Liang, X., Jin, M., ... Xiao, Y. (2020). Genetic structure and insecticide resistance characteristics of fall armyworm populations invading China. Molecular Ecology Resources, 20(6), 1682-1696. https://doi.org/10.1111/1755-0998.13219

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title = "Genetic structure and insecticide resistance characteristics of fall armyworm populations invading China",

abstract = "The rapid wide‐scale spread of fall armyworm (Spodoptera frugiperda ) has caused serious crop losses globally. However, differences in the genetic background of subpopulations and the mechanisms of rapid adaptation behind the invasion are still not well understood. Here we report the assembly of a 390.38Mb chromosome‐level genome of fall armyworm derived from south‐central Africa using Pacific Bioscience (PacBio) and Hi‐C sequencing technologies, with scaffold N50 of 12.9 Mb and containing 22260 annotated protein‐coding genes. Genome‐wide resequencing of 103 samples and strain identification were conducted to reveal the genetic background of fall armyworm populations in China. Analysis of genes related to pesticide‐ and Bt‐resistance showed that the risk of fall armyworm developing resistance to conventional pesticides is very high. Laboratory bioassay results showed that insects invading China carry resistance to organophosphate and pyrethroid pesticides, but are sensitive to genetically modified maize expressing the Bacillus thuringiensis (Bt) toxin Cry1Ab in field experiments. Additionally, two mitochondrial fragments were found to be inserted into the nuclear genome, with the insertion event occurring after the differentiation of the two strains. This study represents a valuable advance toward improving management strategies for fall armyworm.",

keywords = "gene insertion, population structure, resistance risk, Spodoptera frugiperda, subpopulations",

author = "Lei Zhang and Bo Liu and Weigang Zheng and Conghui Liu and Dandan Zhang and Shengyuan Zhao and Zaiyuan Li and Pengjun Xu and Kenneth Wilson and Amy Withers and Chris Jones and Smith, \{Judith A.\} and Gilson Chipabika and Kachigamba, \{Donald L.\} and Kiwoong Nam and Emmanuelle d{\textquoteright}Alen{\c c}on and Bei Liu and Xinyue Liang and Minghui Jin and Chao Wu and Swapan Chakrabarty and Xianming Yang and Yuying Jiang and Jie Liu and Xiaolin Liu and Weipeng Quan and Guirong Wang and Wei Fan and Wanqiang Qian and Kongming Wu and Yutao Xiao",

year = "2020",

month = nov,

day = "1",

doi = "10.1111/1755-0998.13219",

language = "English",

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Zhang, L, Liu, B, Zheng, W, Liu, C, Zhang, D, Zhao, S, Li, Z, Xu, P, Wilson, K, Withers, A, Jones, C, Smith, JA, Chipabika, G, Kachigamba, DL, Nam, K, d’Alençon, E, Liu, B, Liang, X, Jin, M, Wu, C, Chakrabarty, S, Yang, X, Jiang, Y, Liu, J, Liu, X, Quan, W, Wang, G, Fan, W, Qian, W, Wu, K & Xiao, Y 2020, 'Genetic structure and insecticide resistance characteristics of fall armyworm populations invading China', Molecular Ecology Resources, vol. 20, no. 6, pp. 1682-1696. https://doi.org/10.1111/1755-0998.13219

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T1 - Genetic structure and insecticide resistance characteristics of fall armyworm populations invading China

AU - Zhang, Lei

AU - Liu, Bo

AU - Zheng, Weigang

AU - Liu, Conghui

AU - Zhang, Dandan

AU - Zhao, Shengyuan

AU - Li, Zaiyuan

AU - Xu, Pengjun

AU - Wilson, Kenneth

AU - Withers, Amy

AU - Jones, Chris

AU - Smith, Judith A.

AU - Chipabika, Gilson

AU - Kachigamba, Donald L.

AU - Nam, Kiwoong

AU - d’Alençon, Emmanuelle

AU - Liu, Bei

AU - Liang, Xinyue

AU - Jin, Minghui

AU - Wu, Chao

AU - Chakrabarty, Swapan

AU - Yang, Xianming

AU - Jiang, Yuying

AU - Liu, Jie

AU - Liu, Xiaolin

AU - Quan, Weipeng

AU - Wang, Guirong

AU - Fan, Wei

AU - Qian, Wanqiang

AU - Wu, Kongming

AU - Xiao, Yutao

PY - 2020/11/1

Y1 - 2020/11/1

N2 - The rapid wide‐scale spread of fall armyworm (Spodoptera frugiperda ) has caused serious crop losses globally. However, differences in the genetic background of subpopulations and the mechanisms of rapid adaptation behind the invasion are still not well understood. Here we report the assembly of a 390.38Mb chromosome‐level genome of fall armyworm derived from south‐central Africa using Pacific Bioscience (PacBio) and Hi‐C sequencing technologies, with scaffold N50 of 12.9 Mb and containing 22260 annotated protein‐coding genes. Genome‐wide resequencing of 103 samples and strain identification were conducted to reveal the genetic background of fall armyworm populations in China. Analysis of genes related to pesticide‐ and Bt‐resistance showed that the risk of fall armyworm developing resistance to conventional pesticides is very high. Laboratory bioassay results showed that insects invading China carry resistance to organophosphate and pyrethroid pesticides, but are sensitive to genetically modified maize expressing the Bacillus thuringiensis (Bt) toxin Cry1Ab in field experiments. Additionally, two mitochondrial fragments were found to be inserted into the nuclear genome, with the insertion event occurring after the differentiation of the two strains. This study represents a valuable advance toward improving management strategies for fall armyworm.

AB - The rapid wide‐scale spread of fall armyworm (Spodoptera frugiperda ) has caused serious crop losses globally. However, differences in the genetic background of subpopulations and the mechanisms of rapid adaptation behind the invasion are still not well understood. Here we report the assembly of a 390.38Mb chromosome‐level genome of fall armyworm derived from south‐central Africa using Pacific Bioscience (PacBio) and Hi‐C sequencing technologies, with scaffold N50 of 12.9 Mb and containing 22260 annotated protein‐coding genes. Genome‐wide resequencing of 103 samples and strain identification were conducted to reveal the genetic background of fall armyworm populations in China. Analysis of genes related to pesticide‐ and Bt‐resistance showed that the risk of fall armyworm developing resistance to conventional pesticides is very high. Laboratory bioassay results showed that insects invading China carry resistance to organophosphate and pyrethroid pesticides, but are sensitive to genetically modified maize expressing the Bacillus thuringiensis (Bt) toxin Cry1Ab in field experiments. Additionally, two mitochondrial fragments were found to be inserted into the nuclear genome, with the insertion event occurring after the differentiation of the two strains. This study represents a valuable advance toward improving management strategies for fall armyworm.

KW - gene insertion

KW - population structure

KW - resistance risk

KW - Spodoptera frugiperda

KW - subpopulations

U2 - 10.1111/1755-0998.13219

DO - 10.1111/1755-0998.13219

M3 - Article

SN - 1755-098X

VL - 20

SP - 1682

EP - 1696

JO - Molecular Ecology Resources

JF - Molecular Ecology Resources

IS - 6

ER -

Genetic structure and insecticide resistance characteristics of fall armyworm populations invading China

Abstract

Keywords

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