Genetic characterization of Mycobacterium tuberculosis complex isolates circulating in Abuja, Nigeria

Barbara Molina-Moya; Saddiq T. Abdurrahman; Laura I. Madukaji; Michel Kiréopori Gomgnimbou; Lizania Spinasse; Meissiner Gomes-Fernandes; Harrison Magdinier Gomes; Sarah Kacimi; Russell Dacombe; John S. Bimba; Lovett Lawson; Christophe Sola; Luis Cuevas; Jose Dominguez

doi:10.2147/idr.s166986

Genetic characterization of Mycobacterium tuberculosis complex isolates circulating in Abuja, Nigeria

Barbara Molina-Moya, Saddiq T. Abdurrahman, Laura I. Madukaji, Michel Kiréopori Gomgnimbou, Lizania Spinasse, Meissiner Gomes-Fernandes, Harrison Magdinier Gomes, Sarah Kacimi, Russell Dacombe, John S. Bimba, Lovett Lawson, Christophe Sola, Luis Cuevas, Jose Dominguez

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Objective: Nigeria ranks fourth among the high tuberculosis (TB) burden countries. This study describes the prevalence of drug resistance and the genetic diversity of Mycobacterium tuberculosis in Abuja’s Federal Capital Territory.

Materials and methods: Two hundred and seventy-eight consecutive sputum samples were collected from adults with presumptive TB during 2013–2014. DNA was extracted from Löwenstein–Jensen cultures and analyzed for the identification of nontuberculous mycobacteria species, detection of drug resistance with line probe assays, and high-throughput spacer oligonucleotide typing (spoligotyping) using microbead-based hybridization.

Original language	English
Pages (from-to)	1617-1625
Number of pages	9
Journal	Infection and Drug Resistance
Volume	11
DOIs	https://doi.org/10.2147/idr.s166986
Publication status	Published - 1 Oct 2018

Keywords

Isoniazid
Line probe assay
Microbeads
Rifampicin
Spoligotyping
Tuberculosis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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F idr-166986-prevalence-of-multidrug-resistance-and-mycobacterium-tubercu-092818 44872Final published version, 163 KBLicence: CC BY-NC

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Molina-Moya, B., Abdurrahman, S. T., Madukaji, L. I., Gomgnimbou, M. K., Spinasse, L., Gomes-Fernandes, M., Gomes, H. M., Kacimi, S., Dacombe, R., Bimba, J. S., Lawson, L., Sola, C., Cuevas, L., & Dominguez, J. (2018). Genetic characterization of Mycobacterium tuberculosis complex isolates circulating in Abuja, Nigeria. Infection and Drug Resistance, 11, 1617-1625. https://doi.org/10.2147/idr.s166986

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title = "Genetic characterization of Mycobacterium tuberculosis complex isolates circulating in Abuja, Nigeria",

abstract = "Objective: Nigeria ranks fourth among the high tuberculosis (TB) burden countries. This study describes the prevalence of drug resistance and the genetic diversity of Mycobacterium tuberculosis in Abuja{\textquoteright}s Federal Capital Territory.Materials and methods: Two hundred and seventy-eight consecutive sputum samples were collected from adults with presumptive TB during 2013–2014. DNA was extracted from L{\"o}wenstein–Jensen cultures and analyzed for the identification of nontuberculous mycobacteria species, detection of drug resistance with line probe assays, and high-throughput spacer oligonucleotide typing (spoligotyping) using microbead-based hybridization.",

keywords = "Isoniazid, Line probe assay, Microbeads, Rifampicin, Spoligotyping, Tuberculosis",

author = "Barbara Molina-Moya and Abdurrahman, {Saddiq T.} and Madukaji, {Laura I.} and Gomgnimbou, {Michel Kir{\'e}opori} and Lizania Spinasse and Meissiner Gomes-Fernandes and Gomes, {Harrison Magdinier} and Sarah Kacimi and Russell Dacombe and Bimba, {John S.} and Lovett Lawson and Christophe Sola and Luis Cuevas and Jose Dominguez",

year = "2018",

month = oct,

day = "1",

doi = "10.2147/idr.s166986",

language = "English",

volume = "11",

pages = "1617--1625",

journal = "Infection and Drug Resistance",

issn = "1178-6973",

publisher = "Dove Medical Press Ltd.",

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Molina-Moya, B, Abdurrahman, ST, Madukaji, LI, Gomgnimbou, MK, Spinasse, L, Gomes-Fernandes, M, Gomes, HM, Kacimi, S, Dacombe, R, Bimba, JS, Lawson, L, Sola, C, Cuevas, L & Dominguez, J 2018, 'Genetic characterization of Mycobacterium tuberculosis complex isolates circulating in Abuja, Nigeria', Infection and Drug Resistance, vol. 11, pp. 1617-1625. https://doi.org/10.2147/idr.s166986

TY - JOUR

T1 - Genetic characterization of Mycobacterium tuberculosis complex isolates circulating in Abuja, Nigeria

AU - Molina-Moya, Barbara

AU - Abdurrahman, Saddiq T.

AU - Madukaji, Laura I.

AU - Gomgnimbou, Michel Kiréopori

AU - Spinasse, Lizania

AU - Gomes-Fernandes, Meissiner

AU - Gomes, Harrison Magdinier

AU - Kacimi, Sarah

AU - Dacombe, Russell

AU - Bimba, John S.

AU - Lawson, Lovett

AU - Sola, Christophe

AU - Cuevas, Luis

AU - Dominguez, Jose

PY - 2018/10/1

Y1 - 2018/10/1

N2 - Objective: Nigeria ranks fourth among the high tuberculosis (TB) burden countries. This study describes the prevalence of drug resistance and the genetic diversity of Mycobacterium tuberculosis in Abuja’s Federal Capital Territory.Materials and methods: Two hundred and seventy-eight consecutive sputum samples were collected from adults with presumptive TB during 2013–2014. DNA was extracted from Löwenstein–Jensen cultures and analyzed for the identification of nontuberculous mycobacteria species, detection of drug resistance with line probe assays, and high-throughput spacer oligonucleotide typing (spoligotyping) using microbead-based hybridization.

AB - Objective: Nigeria ranks fourth among the high tuberculosis (TB) burden countries. This study describes the prevalence of drug resistance and the genetic diversity of Mycobacterium tuberculosis in Abuja’s Federal Capital Territory.Materials and methods: Two hundred and seventy-eight consecutive sputum samples were collected from adults with presumptive TB during 2013–2014. DNA was extracted from Löwenstein–Jensen cultures and analyzed for the identification of nontuberculous mycobacteria species, detection of drug resistance with line probe assays, and high-throughput spacer oligonucleotide typing (spoligotyping) using microbead-based hybridization.

KW - Isoniazid

KW - Line probe assay

KW - Microbeads

KW - Rifampicin

KW - Spoligotyping

KW - Tuberculosis

U2 - 10.2147/idr.s166986

DO - 10.2147/idr.s166986

M3 - Article

SN - 1178-6973

VL - 11

SP - 1617

EP - 1625

JO - Infection and Drug Resistance

JF - Infection and Drug Resistance

ER -

Genetic characterization of Mycobacterium tuberculosis complex isolates circulating in Abuja, Nigeria

Abstract

Keywords

UN SDGs

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