Gene expression in insecticide resistant and susceptible Anopheles gambiae strains constitutively or after insecticide exposure

J. Vontas; C. Blass; A. C. Koutsos; J. P. David; F. C. Kafatos; C. Louis; Janet Hemingway; G. K. Christophides; Hilary Ranson

doi:10.1111/j.1365-2583.2005.00582.x

Gene expression in insecticide resistant and susceptible Anopheles gambiae strains constitutively or after insecticide exposure

J. Vontas, C. Blass, A. C. Koutsos, J. P. David, F. C. Kafatos, C. Louis, Janet Hemingway, G. K. Christophides, Hilary Ranson

Vector Biology

Research output: Contribution to journal › Article › peer-review

179 Citations (Scopus)

Abstract

A microarray containing approximately 20 000 expressed sequence tags (ESTs; 11 760 unique EST clusters) from the malaria vector, Anopheles gambiae, was used to monitor differences in global gene expression in two insecticide resistant and one susceptible strains. Statistical analysis identified 77 ESTs that were differentially transcribed among the three strains. These include the cytochrome P450 CYP314A1, over-transcribed in the DDT resistant ZAN/U strain, and many genes that belong to families not usually associated with insecticide resistance, such as peptidases, sodium/calcium exchangers and genes implicated in lipid and carbohydrate metabolism. Short-term (6 and 10 h) effects of exposure of the pyrethroid resistant RSP strain to permethrin were also detected. Several genes belonging to enzyme families already implicated in insecticide or xenobiotic detoxification were induced, including the carboxylesterase COEAE2F gene and members of the UDP-glucuronosyl transferase and nitrilase families.

Original language	English
Pages (from-to)	509-521
Number of pages	13
Journal	Insect Molecular Biology
Volume	14
Issue number	5
DOIs	https://doi.org/10.1111/j.1365-2583.2005.00582.x
Publication status	Published - 1 Oct 2005

Keywords

Anopheles gambiae
Detoxification
Insecticide resistance
Microarray

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1111/j.1365-2583.2005.00582.x

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title = "Gene expression in insecticide resistant and susceptible Anopheles gambiae strains constitutively or after insecticide exposure",

abstract = "A microarray containing approximately 20 000 expressed sequence tags (ESTs; 11 760 unique EST clusters) from the malaria vector, Anopheles gambiae, was used to monitor differences in global gene expression in two insecticide resistant and one susceptible strains. Statistical analysis identified 77 ESTs that were differentially transcribed among the three strains. These include the cytochrome P450 CYP314A1, over-transcribed in the DDT resistant ZAN/U strain, and many genes that belong to families not usually associated with insecticide resistance, such as peptidases, sodium/calcium exchangers and genes implicated in lipid and carbohydrate metabolism. Short-term (6 and 10 h) effects of exposure of the pyrethroid resistant RSP strain to permethrin were also detected. Several genes belonging to enzyme families already implicated in insecticide or xenobiotic detoxification were induced, including the carboxylesterase COEAE2F gene and members of the UDP-glucuronosyl transferase and nitrilase families.",

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doi = "10.1111/j.1365-2583.2005.00582.x",

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T1 - Gene expression in insecticide resistant and susceptible Anopheles gambiae strains constitutively or after insecticide exposure

AU - Vontas, J.

AU - Blass, C.

AU - Koutsos, A. C.

AU - David, J. P.

AU - Kafatos, F. C.

AU - Louis, C.

AU - Hemingway, Janet

AU - Christophides, G. K.

AU - Ranson, Hilary

PY - 2005/10/1

Y1 - 2005/10/1

N2 - A microarray containing approximately 20 000 expressed sequence tags (ESTs; 11 760 unique EST clusters) from the malaria vector, Anopheles gambiae, was used to monitor differences in global gene expression in two insecticide resistant and one susceptible strains. Statistical analysis identified 77 ESTs that were differentially transcribed among the three strains. These include the cytochrome P450 CYP314A1, over-transcribed in the DDT resistant ZAN/U strain, and many genes that belong to families not usually associated with insecticide resistance, such as peptidases, sodium/calcium exchangers and genes implicated in lipid and carbohydrate metabolism. Short-term (6 and 10 h) effects of exposure of the pyrethroid resistant RSP strain to permethrin were also detected. Several genes belonging to enzyme families already implicated in insecticide or xenobiotic detoxification were induced, including the carboxylesterase COEAE2F gene and members of the UDP-glucuronosyl transferase and nitrilase families.

AB - A microarray containing approximately 20 000 expressed sequence tags (ESTs; 11 760 unique EST clusters) from the malaria vector, Anopheles gambiae, was used to monitor differences in global gene expression in two insecticide resistant and one susceptible strains. Statistical analysis identified 77 ESTs that were differentially transcribed among the three strains. These include the cytochrome P450 CYP314A1, over-transcribed in the DDT resistant ZAN/U strain, and many genes that belong to families not usually associated with insecticide resistance, such as peptidases, sodium/calcium exchangers and genes implicated in lipid and carbohydrate metabolism. Short-term (6 and 10 h) effects of exposure of the pyrethroid resistant RSP strain to permethrin were also detected. Several genes belonging to enzyme families already implicated in insecticide or xenobiotic detoxification were induced, including the carboxylesterase COEAE2F gene and members of the UDP-glucuronosyl transferase and nitrilase families.

KW - Anopheles gambiae

KW - Detoxification

KW - Insecticide resistance

KW - Microarray

U2 - 10.1111/j.1365-2583.2005.00582.x

DO - 10.1111/j.1365-2583.2005.00582.x

M3 - Article

SN - 0962-1075

VL - 14

SP - 509

EP - 521

JO - Insect Molecular Biology

JF - Insect Molecular Biology

IS - 5

ER -

Gene expression in insecticide resistant and susceptible Anopheles gambiae strains constitutively or after insecticide exposure

Abstract

Keywords

UN SDGs

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