Gene expression in Echis carinatus (carpet viper) venom glands following milking

Mark Paine; H. P. Desmond; R. D.G. Theakston; J. M. Crampton

doi:10.1016/0041-0101(92)90534-c

Gene expression in Echis carinatus (carpet viper) venom glands following milking

Mark Paine, H. P. Desmond, R. D.G. Theakston, J. M. Crampton

Vector Biology

Liverpool School of Tropical Medicine

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

Abstract

RNA was extracted from the venom glands of Echis carinatus at different times after milking, and the temporal pattern and nature of mRNA transcribed during venom regeneration was examined by in vitro translation. Venom products were immunoprecipitated with E. carinatus venom polyclonal antiserum. Maximum transcriptional activity occurred 3 days after milking. Electrophoretic analysis of the translation products showed minimal differences in the banding patterns at each time interval. Analysis of the translation products from Kenyan, Nigerian and Saudi Arabian carpet vipers, however, revealed differences which suggest that the observed heterogeneity in E. carinatus venom occurs at the level of the genome.

Original language	English
Pages (from-to)	379-386
Number of pages	8
Journal	Toxicon
Volume	30
Issue number	4
DOIs	https://doi.org/10.1016/0041-0101(92)90534-c
Publication status	Published - 1 Apr 1992

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title = "Gene expression in Echis carinatus (carpet viper) venom glands following milking",

abstract = "RNA was extracted from the venom glands of Echis carinatus at different times after milking, and the temporal pattern and nature of mRNA transcribed during venom regeneration was examined by in vitro translation. Venom products were immunoprecipitated with E. carinatus venom polyclonal antiserum. Maximum transcriptional activity occurred 3 days after milking. Electrophoretic analysis of the translation products showed minimal differences in the banding patterns at each time interval. Analysis of the translation products from Kenyan, Nigerian and Saudi Arabian carpet vipers, however, revealed differences which suggest that the observed heterogeneity in E. carinatus venom occurs at the level of the genome.",

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N2 - RNA was extracted from the venom glands of Echis carinatus at different times after milking, and the temporal pattern and nature of mRNA transcribed during venom regeneration was examined by in vitro translation. Venom products were immunoprecipitated with E. carinatus venom polyclonal antiserum. Maximum transcriptional activity occurred 3 days after milking. Electrophoretic analysis of the translation products showed minimal differences in the banding patterns at each time interval. Analysis of the translation products from Kenyan, Nigerian and Saudi Arabian carpet vipers, however, revealed differences which suggest that the observed heterogeneity in E. carinatus venom occurs at the level of the genome.

AB - RNA was extracted from the venom glands of Echis carinatus at different times after milking, and the temporal pattern and nature of mRNA transcribed during venom regeneration was examined by in vitro translation. Venom products were immunoprecipitated with E. carinatus venom polyclonal antiserum. Maximum transcriptional activity occurred 3 days after milking. Electrophoretic analysis of the translation products showed minimal differences in the banding patterns at each time interval. Analysis of the translation products from Kenyan, Nigerian and Saudi Arabian carpet vipers, however, revealed differences which suggest that the observed heterogeneity in E. carinatus venom occurs at the level of the genome.

U2 - 10.1016/0041-0101(92)90534-c

DO - 10.1016/0041-0101(92)90534-c

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JO - Toxicon

JF - Toxicon

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Gene expression in Echis carinatus (carpet viper) venom glands following milking

Abstract

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