Functional Evidence for Oxygen-Sensitive Voltage-Gated Potassium Channels in Human Placental Vasculature

M. F. Kiernan; A. Barrie; J. Szkolar; T. A. Mills; M. Wareing

doi:10.1016/j.placenta.2010.03.009

Functional Evidence for Oxygen-Sensitive Voltage-Gated Potassium Channels in Human Placental Vasculature

M. F. Kiernan, A. Barrie, J. Szkolar, T. A. Mills, M. Wareing

University of Manchester

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

Hypoxic fetoplacental vasoconstriction (HFPV), involving voltage-gated potassium (K_V) channels, has been suggested in human placenta; the identity of these channels remains unclear. Using wire myography, chorionic plate blood vessels were exposed to isoform-specific K_V channel blockers. Dose-response curves (thromboxane mimetic U46619; 0.1-2000 nM) pre- and post-addition of K_V channel modulator were analysed. Arterial U46619-induced contraction increased with margatoxin and stromatoxin-1, whilst only correolide increased U46619-induced contraction in veins (P < 0.05 two-way ANOVA). Basal tone was unaffected in arteries or veins. These data implicate K_V1.2 and/or K_V2.1 and K_V1.5 in the control of agonist-induced contraction of human placental arteries and veins respectively.

Original language	English
Pages (from-to)	553-555
Number of pages	3
Journal	Placenta
Volume	31
Issue number	6
DOIs	https://doi.org/10.1016/j.placenta.2010.03.009
Publication status	Published - 6 Jun 2010
Externally published	Yes

Keywords

Human
Placenta vasculature
Potassium channel

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.placenta.2010.03.009

Cite this

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title = "Functional Evidence for Oxygen-Sensitive Voltage-Gated Potassium Channels in Human Placental Vasculature",

abstract = "Hypoxic fetoplacental vasoconstriction (HFPV), involving voltage-gated potassium (KV) channels, has been suggested in human placenta; the identity of these channels remains unclear. Using wire myography, chorionic plate blood vessels were exposed to isoform-specific KV channel blockers. Dose-response curves (thromboxane mimetic U46619; 0.1-2000 nM) pre- and post-addition of KV channel modulator were analysed. Arterial U46619-induced contraction increased with margatoxin and stromatoxin-1, whilst only correolide increased U46619-induced contraction in veins (P < 0.05 two-way ANOVA). Basal tone was unaffected in arteries or veins. These data implicate KV1.2 and/or KV2.1 and KV1.5 in the control of agonist-induced contraction of human placental arteries and veins respectively.",

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doi = "10.1016/j.placenta.2010.03.009",

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T1 - Functional Evidence for Oxygen-Sensitive Voltage-Gated Potassium Channels in Human Placental Vasculature

AU - Kiernan, M. F.

AU - Barrie, A.

AU - Szkolar, J.

AU - Mills, T. A.

AU - Wareing, M.

PY - 2010/6/6

Y1 - 2010/6/6

N2 - Hypoxic fetoplacental vasoconstriction (HFPV), involving voltage-gated potassium (KV) channels, has been suggested in human placenta; the identity of these channels remains unclear. Using wire myography, chorionic plate blood vessels were exposed to isoform-specific KV channel blockers. Dose-response curves (thromboxane mimetic U46619; 0.1-2000 nM) pre- and post-addition of KV channel modulator were analysed. Arterial U46619-induced contraction increased with margatoxin and stromatoxin-1, whilst only correolide increased U46619-induced contraction in veins (P < 0.05 two-way ANOVA). Basal tone was unaffected in arteries or veins. These data implicate KV1.2 and/or KV2.1 and KV1.5 in the control of agonist-induced contraction of human placental arteries and veins respectively.

AB - Hypoxic fetoplacental vasoconstriction (HFPV), involving voltage-gated potassium (KV) channels, has been suggested in human placenta; the identity of these channels remains unclear. Using wire myography, chorionic plate blood vessels were exposed to isoform-specific KV channel blockers. Dose-response curves (thromboxane mimetic U46619; 0.1-2000 nM) pre- and post-addition of KV channel modulator were analysed. Arterial U46619-induced contraction increased with margatoxin and stromatoxin-1, whilst only correolide increased U46619-induced contraction in veins (P < 0.05 two-way ANOVA). Basal tone was unaffected in arteries or veins. These data implicate KV1.2 and/or KV2.1 and KV1.5 in the control of agonist-induced contraction of human placental arteries and veins respectively.

KW - Human

KW - Placenta vasculature

KW - Potassium channel

U2 - 10.1016/j.placenta.2010.03.009

DO - 10.1016/j.placenta.2010.03.009

M3 - Article

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SN - 0143-4004

VL - 31

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JO - Placenta

JF - Placenta

IS - 6

ER -

Functional Evidence for Oxygen-Sensitive Voltage-Gated Potassium Channels in Human Placental Vasculature

Abstract

Keywords

UN SDGs

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