Functional and Sequence-Specific Screening Protocols for the Detection of Novel Antimicrobial Resistance Genes in Metagenomic DNA

Supathep Tansirichaiya; William Hutton; Adam Roberts

doi:10.1007/978-1-0716-2795-2_4

Functional and Sequence-Specific Screening Protocols for the Detection of Novel Antimicrobial Resistance Genes in Metagenomic DNA

Supathep Tansirichaiya, William Hutton, Adam Roberts

Mahidol University

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

3 Citations (Scopus)

Abstract

Antimicrobial resistance (AMR) is an increasingly important global challenge for healthcare systems as well as agricultural food production systems. Our ability to prepare for, and respond to, emerging AMR threats is dependent on our knowledge of genes able to confer AMR that are circulating within various environmental, animal, and human microbiomes. Targeted, sequence-specific, detection of AMR genes and functional resistance assays, described here, carried out on metagenomic DNA gives us unique insights into the presence of AMR genes and how these are associated with mobile genetic elements that may be responsible for their dissemination and can also provide important information about the mechanisms of resistance underpinning the phenotype.

Original language	English
Title of host publication	Metagenomics
Publisher	Springer Nature
Pages	51-72
Number of pages	22
DOIs	https://doi.org/10.1007/978-1-0716-2795-2_4
Publication status	Published - 29 Oct 2022

Keywords

AMR genes
Antimicrobial resistance
Functional screening
Metagenomic DNA
Oral microbiome
Sequence-specific screening

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/978-1-0716-2795-2_4

Natural Drivers of Antimicrobial Resistance
Hutton, W. (Author), Roberts, A. (Supervisor) & Singer, A. (Supervisor), 2022
Student thesis: Doctoral thesis

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Cite this

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AB - Antimicrobial resistance (AMR) is an increasingly important global challenge for healthcare systems as well as agricultural food production systems. Our ability to prepare for, and respond to, emerging AMR threats is dependent on our knowledge of genes able to confer AMR that are circulating within various environmental, animal, and human microbiomes. Targeted, sequence-specific, detection of AMR genes and functional resistance assays, described here, carried out on metagenomic DNA gives us unique insights into the presence of AMR genes and how these are associated with mobile genetic elements that may be responsible for their dissemination and can also provide important information about the mechanisms of resistance underpinning the phenotype.

KW - AMR genes

KW - Antimicrobial resistance

KW - Functional screening

KW - Metagenomic DNA

KW - Oral microbiome

KW - Sequence-specific screening

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M3 - Chapter

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EP - 72

BT - Metagenomics

PB - Springer Nature

ER -

Functional and Sequence-Specific Screening Protocols for the Detection of Novel Antimicrobial Resistance Genes in Metagenomic DNA

Abstract

Keywords

UN SDGs

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Student theses

Natural Drivers of Antimicrobial Resistance

Cite this