Field evaluation of a Pan-Lassa rapid diagnostic test during the 2018 Nigerian Lassa fever outbreak

Matthew L. Boisen; Eghosa Uyigue; John Aiyepada; Katherine J. Siddle; Lisa Oestereich; Diana K.S. Nelson; Duane J. Bush; Megan M. Rowland; Megan L. Heinrich; Philomena Eromon; Adeyemi T. Kayode; Ikponmwosa Odia; Donatus I. Adomeh; Ekene B. Muoebonam; Patience Akhilomen; Grace Okonofua; Blessing Osiemi; Omigie Omoregie; Michael Airende; Jacqueline Agbukor; Solomon Ehikhametalor; Chris Okafi Aire; Sophie Duraffour; Meike Pahlmann; Wiebke Böhm; Kayla Barnes; Samar Mehta; Mambu Momoh; John Demby Sandi; Augustine Goba; Onikepe A. Folarin; Ephraim Ogbaini-Emovan; Danny A. Asogun; Ekaete A. Tobin; George O. Akpede; Sylvanus A. Okogbenin; Peter O. Okokhere; Donald S. Grant; John S. Schieffelin; Pardis C. Sabeti; Stephan Günther; Christian T. Happi; Luis M. Branco; Robert F. Garry

doi:10.1038/s41598-020-65736-0

Field evaluation of a Pan-Lassa rapid diagnostic test during the 2018 Nigerian Lassa fever outbreak

Matthew L. Boisen, Eghosa Uyigue, John Aiyepada, Katherine J. Siddle, Lisa Oestereich, Diana K.S. Nelson, Duane J. Bush, Megan M. Rowland, Megan L. Heinrich, Philomena Eromon, Adeyemi T. Kayode, Ikponmwosa Odia, Donatus I. Adomeh, Ekene B. Muoebonam, Patience Akhilomen, Grace Okonofua, Blessing Osiemi, Omigie Omoregie, Michael Airende, Jacqueline AgbukorSolomon Ehikhametalor, Chris Okafi Aire, Sophie Duraffour, Meike Pahlmann, Wiebke Böhm, Kayla Barnes, Samar Mehta, Mambu Momoh, John Demby Sandi, Augustine Goba, Onikepe A. Folarin, Ephraim Ogbaini-Emovan, Danny A. Asogun, Ekaete A. Tobin, George O. Akpede, Sylvanus A. Okogbenin, Peter O. Okokhere, Donald S. Grant, John S. Schieffelin, Pardis C. Sabeti, Stephan Günther, Christian T. Happi, Luis M. Branco, Robert F. Garry

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

Lassa virus (LASV) is the causative agent of Lassa fever (LF), an often-fatal hemorrhagic disease. LF is endemic in Nigeria, Sierra Leone and other West African countries. Diagnosis of LASV infection is challenged by the genetic diversity of the virus, which is greatest in Nigeria. The ReLASV Pan-Lassa Antigen Rapid Test (Pan-Lassa RDT) is a point-of-care, in vitro diagnostic test that utilizes a mixture of polyclonal antibodies raised against recombinant nucleoproteins of representative strains from the three most prevalent LASV lineages (II, III and IV). We compared the performance of the Pan-LASV RDT to available quantitative PCR (qPCR) assays during the 2018 LF outbreak in Nigeria. For patients with acute LF (RDT positive, IgG/IgM negative) during initial screening, RDT performance was 83.3% sensitivity and 92.8% specificity when compared to composite results of two qPCR assays. 100% of samples that gave Ct values below 22 on both qPCR assays were positive on the Pan-Lassa RDT. There were significantly elevated case fatality rates and elevated liver transaminase levels in subjects whose samples were RDT positive compared to RDT negative.

Original language	English
Article number	8724
Journal	Scientific Reports
Volume	10
Issue number	1
DOIs	https://doi.org/10.1038/s41598-020-65736-0
Publication status	Published - 1 Dec 2020
Externally published	Yes

Access to Document

10.1038/s41598-020-65736-0Licence: CC BY

Cite this

Boisen, M. L., Uyigue, E., Aiyepada, J., Siddle, K. J., Oestereich, L., Nelson, D. K. S., Bush, D. J., Rowland, M. M., Heinrich, M. L., Eromon, P., Kayode, A. T., Odia, I., Adomeh, D. I., Muoebonam, E. B., Akhilomen, P., Okonofua, G., Osiemi, B., Omoregie, O., Airende, M., ... Garry, R. F. (2020). Field evaluation of a Pan-Lassa rapid diagnostic test during the 2018 Nigerian Lassa fever outbreak. Scientific Reports, 10(1), Article 8724. https://doi.org/10.1038/s41598-020-65736-0

@article{533ae2752e894878ab37efeaa16ac4ad,

title = "Field evaluation of a Pan-Lassa rapid diagnostic test during the 2018 Nigerian Lassa fever outbreak",

abstract = "Lassa virus (LASV) is the causative agent of Lassa fever (LF), an often-fatal hemorrhagic disease. LF is endemic in Nigeria, Sierra Leone and other West African countries. Diagnosis of LASV infection is challenged by the genetic diversity of the virus, which is greatest in Nigeria. The ReLASV Pan-Lassa Antigen Rapid Test (Pan-Lassa RDT) is a point-of-care, in vitro diagnostic test that utilizes a mixture of polyclonal antibodies raised against recombinant nucleoproteins of representative strains from the three most prevalent LASV lineages (II, III and IV). We compared the performance of the Pan-LASV RDT to available quantitative PCR (qPCR) assays during the 2018 LF outbreak in Nigeria. For patients with acute LF (RDT positive, IgG/IgM negative) during initial screening, RDT performance was 83.3\% sensitivity and 92.8\% specificity when compared to composite results of two qPCR assays. 100\% of samples that gave Ct values below 22 on both qPCR assays were positive on the Pan-Lassa RDT. There were significantly elevated case fatality rates and elevated liver transaminase levels in subjects whose samples were RDT positive compared to RDT negative.",

author = "Boisen, \{Matthew L.\} and Eghosa Uyigue and John Aiyepada and Siddle, \{Katherine J.\} and Lisa Oestereich and Nelson, \{Diana K.S.\} and Bush, \{Duane J.\} and Rowland, \{Megan M.\} and Heinrich, \{Megan L.\} and Philomena Eromon and Kayode, \{Adeyemi T.\} and Ikponmwosa Odia and Adomeh, \{Donatus I.\} and Muoebonam, \{Ekene B.\} and Patience Akhilomen and Grace Okonofua and Blessing Osiemi and Omigie Omoregie and Michael Airende and Jacqueline Agbukor and Solomon Ehikhametalor and Aire, \{Chris Okafi\} and Sophie Duraffour and Meike Pahlmann and Wiebke B{\"o}hm and Kayla Barnes and Samar Mehta and Mambu Momoh and Sandi, \{John Demby\} and Augustine Goba and Folarin, \{Onikepe A.\} and Ephraim Ogbaini-Emovan and Asogun, \{Danny A.\} and Tobin, \{Ekaete A.\} and Akpede, \{George O.\} and Okogbenin, \{Sylvanus A.\} and Okokhere, \{Peter O.\} and Grant, \{Donald S.\} and Schieffelin, \{John S.\} and Sabeti, \{Pardis C.\} and Stephan G{\"u}nther and Happi, \{Christian T.\} and Branco, \{Luis M.\} and Garry, \{Robert F.\}",

year = "2020",

month = dec,

day = "1",

doi = "10.1038/s41598-020-65736-0",

language = "English",

volume = "10",

journal = "Scientific Reports",

issn = "2045-2322",

publisher = "Nature Publishing Group",

number = "1",

}

Boisen, ML, Uyigue, E, Aiyepada, J, Siddle, KJ, Oestereich, L, Nelson, DKS, Bush, DJ, Rowland, MM, Heinrich, ML, Eromon, P, Kayode, AT, Odia, I, Adomeh, DI, Muoebonam, EB, Akhilomen, P, Okonofua, G, Osiemi, B, Omoregie, O, Airende, M, Agbukor, J, Ehikhametalor, S, Aire, CO, Duraffour, S, Pahlmann, M, Böhm, W, Barnes, K, Mehta, S, Momoh, M, Sandi, JD, Goba, A, Folarin, OA, Ogbaini-Emovan, E, Asogun, DA, Tobin, EA, Akpede, GO, Okogbenin, SA, Okokhere, PO, Grant, DS, Schieffelin, JS, Sabeti, PC, Günther, S, Happi, CT, Branco, LM & Garry, RF 2020, 'Field evaluation of a Pan-Lassa rapid diagnostic test during the 2018 Nigerian Lassa fever outbreak', Scientific Reports, vol. 10, no. 1, 8724. https://doi.org/10.1038/s41598-020-65736-0

TY - JOUR

T1 - Field evaluation of a Pan-Lassa rapid diagnostic test during the 2018 Nigerian Lassa fever outbreak

AU - Boisen, Matthew L.

AU - Uyigue, Eghosa

AU - Aiyepada, John

AU - Siddle, Katherine J.

AU - Oestereich, Lisa

AU - Nelson, Diana K.S.

AU - Bush, Duane J.

AU - Rowland, Megan M.

AU - Heinrich, Megan L.

AU - Eromon, Philomena

AU - Kayode, Adeyemi T.

AU - Odia, Ikponmwosa

AU - Adomeh, Donatus I.

AU - Muoebonam, Ekene B.

AU - Akhilomen, Patience

AU - Okonofua, Grace

AU - Osiemi, Blessing

AU - Omoregie, Omigie

AU - Airende, Michael

AU - Agbukor, Jacqueline

AU - Ehikhametalor, Solomon

AU - Aire, Chris Okafi

AU - Duraffour, Sophie

AU - Pahlmann, Meike

AU - Böhm, Wiebke

AU - Barnes, Kayla

AU - Mehta, Samar

AU - Momoh, Mambu

AU - Sandi, John Demby

AU - Goba, Augustine

AU - Folarin, Onikepe A.

AU - Ogbaini-Emovan, Ephraim

AU - Asogun, Danny A.

AU - Tobin, Ekaete A.

AU - Akpede, George O.

AU - Okogbenin, Sylvanus A.

AU - Okokhere, Peter O.

AU - Grant, Donald S.

AU - Schieffelin, John S.

AU - Sabeti, Pardis C.

AU - Günther, Stephan

AU - Happi, Christian T.

AU - Branco, Luis M.

AU - Garry, Robert F.

PY - 2020/12/1

Y1 - 2020/12/1

N2 - Lassa virus (LASV) is the causative agent of Lassa fever (LF), an often-fatal hemorrhagic disease. LF is endemic in Nigeria, Sierra Leone and other West African countries. Diagnosis of LASV infection is challenged by the genetic diversity of the virus, which is greatest in Nigeria. The ReLASV Pan-Lassa Antigen Rapid Test (Pan-Lassa RDT) is a point-of-care, in vitro diagnostic test that utilizes a mixture of polyclonal antibodies raised against recombinant nucleoproteins of representative strains from the three most prevalent LASV lineages (II, III and IV). We compared the performance of the Pan-LASV RDT to available quantitative PCR (qPCR) assays during the 2018 LF outbreak in Nigeria. For patients with acute LF (RDT positive, IgG/IgM negative) during initial screening, RDT performance was 83.3% sensitivity and 92.8% specificity when compared to composite results of two qPCR assays. 100% of samples that gave Ct values below 22 on both qPCR assays were positive on the Pan-Lassa RDT. There were significantly elevated case fatality rates and elevated liver transaminase levels in subjects whose samples were RDT positive compared to RDT negative.

AB - Lassa virus (LASV) is the causative agent of Lassa fever (LF), an often-fatal hemorrhagic disease. LF is endemic in Nigeria, Sierra Leone and other West African countries. Diagnosis of LASV infection is challenged by the genetic diversity of the virus, which is greatest in Nigeria. The ReLASV Pan-Lassa Antigen Rapid Test (Pan-Lassa RDT) is a point-of-care, in vitro diagnostic test that utilizes a mixture of polyclonal antibodies raised against recombinant nucleoproteins of representative strains from the three most prevalent LASV lineages (II, III and IV). We compared the performance of the Pan-LASV RDT to available quantitative PCR (qPCR) assays during the 2018 LF outbreak in Nigeria. For patients with acute LF (RDT positive, IgG/IgM negative) during initial screening, RDT performance was 83.3% sensitivity and 92.8% specificity when compared to composite results of two qPCR assays. 100% of samples that gave Ct values below 22 on both qPCR assays were positive on the Pan-Lassa RDT. There were significantly elevated case fatality rates and elevated liver transaminase levels in subjects whose samples were RDT positive compared to RDT negative.

U2 - 10.1038/s41598-020-65736-0

DO - 10.1038/s41598-020-65736-0

M3 - Article

SN - 2045-2322

VL - 10

JO - Scientific Reports

JF - Scientific Reports

IS - 1

M1 - 8724

ER -

Field evaluation of a Pan-Lassa rapid diagnostic test during the 2018 Nigerian Lassa fever outbreak

Abstract

Access to Document

Fingerprint

Cite this