Abstract
Full-length cDNA clones encoding D7 (AnsD7) and D7-related (AnsD7r1) secreted salivary gland proteins were isolated from Anopheles stephensi. Corresponding proteins were separated by SDS-PAGE and analysed by N-terminal sequencing, which also identified a second D7-related protein (AnsD7r2). AnsD7 encodes a protein of 37 kDa, AnsD7r1 of 18 kDa, and AnsD7r2 of 16 kDa. Polyclonal antibodies against recombinant AnsD7 showed immunological cross-reactivity with the D7-related proteins, and alignment demonstrated sequence similarity between the C-terminal region of AnsD7 and the D7-related proteins. AnsD7, AnsD7r1 and AnsD7r2 were major female-specific salivary gland proteins, and Western blotting, immunohistochemistry and immunogold labelling demonstrated expression was predominantly in the secretory cavities of the distal-lateral and median lobes. Expression and localization of D7 and D7-related proteins was similar in Plasmodium, berghei-infected and uninfected mosquitoes.
| Original language | English |
|---|---|
| Pages (from-to) | 223-232 |
| Number of pages | 10 |
| Journal | Insect Molecular Biology |
| Volume | 11 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1 Jun 2002 |
Keywords
- Anopheles stephensi
- Malaria
- Mosquito
- Salivary glands
