Experimental Transmission of Plasmodium malariae to Anopheles gambiae

Yudi T. Pinilla; Stravensky T. Boussougou-Sambe; Sarah Gräßle; Barclaye Ngossanga; Ange G. Doumba-Ndalembouly; Andrea Weierich; Gedeon Bingoulou; Emma G. Malinga; Daniel Nguiffo-Nguete; Francine Ntoumi; Luc Djogbénou; Saadou Issifou; Charles Wondji; Ayola A. Adegnika; Steffen Borrmann; Theo Nzoughe-Nzeng; Jean Ronald Edoa; Elsy Dansou N'Noh; Jeannot Zinsou; Cyrille Ndo; Francis Nkemngo; Magellan Tchouakoui; Williams Tchapga; Renette Ayuk; Jacques Mbama Ntabi; Felix Koukouikila-Koussounda; Romuald Agonhossou; Romaric Bidossessi Akoton; Yannelle Dossou Akpeyedje; Katharina Beck; Nathanael Saison; Anton Hoffmann

doi:10.1093/infdis/jiaa382

Experimental Transmission of Plasmodium malariae to Anopheles gambiae

Yudi T. Pinilla, Stravensky T. Boussougou-Sambe, Sarah Gräßle, Barclaye Ngossanga, Ange G. Doumba-Ndalembouly, Andrea Weierich, Gedeon Bingoulou, Emma G. Malinga, Daniel Nguiffo-Nguete, Francine Ntoumi, Luc Djogbénou, Saadou Issifou, Charles Wondji, Ayola A. Adegnika, Steffen Borrmann, Theo Nzoughe-Nzeng, Jean Ronald Edoa, Elsy Dansou N'Noh, Jeannot Zinsou, Cyrille NdoFrancis Nkemngo, Magellan Tchouakoui, Williams Tchapga, Renette Ayuk, Jacques Mbama Ntabi, Felix Koukouikila-Koussounda, Romuald Agonhossou, Romaric Bidossessi Akoton, Yannelle Dossou Akpeyedje, Katharina Beck, Nathanael Saison, Anton Hoffmann

Vector Biology

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Our current knowledge of the clinical burden, biology, and

transmission of Plasmodium malariae is extremely scarce. To

start addressing some of those questions, we experimentally

infected Anopheles gambiae mosquitoes with fresh P. malariae

isolates obtained from asymptomatic individuals in Lambaréné,

Gabon. The proportion of mosquitoes infected via direct membrane

feeding assay with either P. malariae monoinfections

(16% [19 of 121]) or coinfections (28% [31 of 112]) was higher

after serum replacement than in parallel groups without serum

replacement (4% [4 of 102] and 4% [2 of 45], respectively;

P < .01). Our results show that isolates from asymptomatic

carriers can be used for experimental studies of P. malariae

transmission.

Original language	English
Pages (from-to)	522-526
Number of pages	5
Journal	Journal of Infectious Diseases
Volume	223
Issue number	3
Early online date	4 Jul 2020
DOIs	https://doi.org/10.1093/infdis/jiaa382
Publication status	Published - 1 Feb 2021

Keywords

Anopheles gambiae
experimental transmission
Gabon
Plasmodium malariae

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Pinilla, Y. T., Boussougou-Sambe, S. T., Gräßle, S., Ngossanga, B., Doumba-Ndalembouly, A. G., Weierich, A., Bingoulou, G., Malinga, E. G., Nguiffo-Nguete, D., Ntoumi, F., Djogbénou, L., Issifou, S., Wondji, C., Adegnika, A. A., Borrmann, S., Nzoughe-Nzeng, T., Edoa, J. R., N'Noh, E. D., Zinsou, J., ... Hoffmann, A. (2021). Experimental Transmission of Plasmodium malariae to Anopheles gambiae. Journal of Infectious Diseases, 223(3), 522-526. https://doi.org/10.1093/infdis/jiaa382

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abstract = "Our current knowledge of the clinical burden, biology, andtransmission of Plasmodium malariae is extremely scarce. Tostart addressing some of those questions, we experimentallyinfected Anopheles gambiae mosquitoes with fresh P. malariaeisolates obtained from asymptomatic individuals in Lambar{\'e}n{\'e},Gabon. The proportion of mosquitoes infected via direct membranefeeding assay with either P. malariae monoinfections(16\% [19 of 121]) or coinfections (28\% [31 of 112]) was higherafter serum replacement than in parallel groups without serumreplacement (4\% [4 of 102] and 4\% [2 of 45], respectively;P < .01). Our results show that isolates from asymptomaticcarriers can be used for experimental studies of P. malariaetransmission.",

keywords = "Anopheles gambiae, experimental transmission, Gabon, Plasmodium malariae",

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Pinilla, YT, Boussougou-Sambe, ST, Gräßle, S, Ngossanga, B, Doumba-Ndalembouly, AG, Weierich, A, Bingoulou, G, Malinga, EG, Nguiffo-Nguete, D, Ntoumi, F, Djogbénou, L, Issifou, S, Wondji, C, Adegnika, AA, Borrmann, S, Nzoughe-Nzeng, T, Edoa, JR, N'Noh, ED, Zinsou, J, Ndo, C, Nkemngo, F, Tchouakoui, M, Tchapga, W, Ayuk, R, Ntabi, JM, Koukouikila-Koussounda, F, Agonhossou, R, Akoton, RB, Akpeyedje, YD, Beck, K, Saison, N & Hoffmann, A 2021, 'Experimental Transmission of Plasmodium malariae to Anopheles gambiae', Journal of Infectious Diseases, vol. 223, no. 3, pp. 522-526. https://doi.org/10.1093/infdis/jiaa382

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T1 - Experimental Transmission of Plasmodium malariae to Anopheles gambiae

AU - Pinilla, Yudi T.

AU - Boussougou-Sambe, Stravensky T.

AU - Gräßle, Sarah

AU - Ngossanga, Barclaye

AU - Doumba-Ndalembouly, Ange G.

AU - Weierich, Andrea

AU - Bingoulou, Gedeon

AU - Malinga, Emma G.

AU - Nguiffo-Nguete, Daniel

AU - Ntoumi, Francine

AU - Djogbénou, Luc

AU - Issifou, Saadou

AU - Wondji, Charles

AU - Adegnika, Ayola A.

AU - Borrmann, Steffen

AU - Nzoughe-Nzeng, Theo

AU - Edoa, Jean Ronald

AU - N'Noh, Elsy Dansou

AU - Zinsou, Jeannot

AU - Ndo, Cyrille

AU - Nkemngo, Francis

AU - Tchouakoui, Magellan

AU - Tchapga, Williams

AU - Ayuk, Renette

AU - Ntabi, Jacques Mbama

AU - Koukouikila-Koussounda, Felix

AU - Agonhossou, Romuald

AU - Akoton, Romaric Bidossessi

AU - Akpeyedje, Yannelle Dossou

AU - Beck, Katharina

AU - Saison, Nathanael

AU - Hoffmann, Anton

PY - 2021/2/1

Y1 - 2021/2/1

N2 - Our current knowledge of the clinical burden, biology, andtransmission of Plasmodium malariae is extremely scarce. Tostart addressing some of those questions, we experimentallyinfected Anopheles gambiae mosquitoes with fresh P. malariaeisolates obtained from asymptomatic individuals in Lambaréné,Gabon. The proportion of mosquitoes infected via direct membranefeeding assay with either P. malariae monoinfections(16% [19 of 121]) or coinfections (28% [31 of 112]) was higherafter serum replacement than in parallel groups without serumreplacement (4% [4 of 102] and 4% [2 of 45], respectively;P < .01). Our results show that isolates from asymptomaticcarriers can be used for experimental studies of P. malariaetransmission.

AB - Our current knowledge of the clinical burden, biology, andtransmission of Plasmodium malariae is extremely scarce. Tostart addressing some of those questions, we experimentallyinfected Anopheles gambiae mosquitoes with fresh P. malariaeisolates obtained from asymptomatic individuals in Lambaréné,Gabon. The proportion of mosquitoes infected via direct membranefeeding assay with either P. malariae monoinfections(16% [19 of 121]) or coinfections (28% [31 of 112]) was higherafter serum replacement than in parallel groups without serumreplacement (4% [4 of 102] and 4% [2 of 45], respectively;P < .01). Our results show that isolates from asymptomaticcarriers can be used for experimental studies of P. malariaetransmission.

KW - Anopheles gambiae

KW - experimental transmission

KW - Gabon

KW - Plasmodium malariae

U2 - 10.1093/infdis/jiaa382

DO - 10.1093/infdis/jiaa382

M3 - Article

SN - 0022-1899

VL - 223

SP - 522

EP - 526

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

IS - 3

ER -

Experimental Transmission of Plasmodium malariae to Anopheles gambiae

Abstract

Keywords

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