Experimental huts trial of the efficacy of pyrethroids/piperonyl butoxide (PBO) net treatments for controlling multi-resistant populations of Anopheles funestus s.s. in Kpomè, Southern Benin

Romaric Akoton; Genevieve M. Tchigossou; Innocent Djègbè; Akadiri Yessoufou; Michael Seun Atoyebi; Eric Tossou; Francis Zeukeng; Pelagie Boko; Helen Irving; Razack Adéoti; Jacob Riveron; Charles Wondji; Kabirou Moutairou; Rousseau Djouaka

doi:10.12688/wellcomeopenres.14589.1

Experimental huts trial of the efficacy of pyrethroids/piperonyl butoxide (PBO) net treatments for controlling multi-resistant populations of Anopheles funestus s.s. in Kpomè, Southern Benin

Romaric Akoton
, Genevieve M. Tchigossou
, Innocent Djègbè
, Akadiri Yessoufou
, Michael Seun Atoyebi
, Eric Tossou
, Francis Zeukeng
, Pelagie Boko
, Helen Irving
, Razack Adéoti
, Jacob Riveron
, Charles Wondji
, Kabirou Moutairou
, Rousseau Djouaka

Vector Biology

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Background: Insecticides resistance in Anopheles mosquitoes limits Long-Lasting Insecticidal Nets (LLIN) used for malaria control in Africa, especially Benin. This study aimed to evaluate the bio-efficacy of current LLINs in an area where An. funestus s.l. and An. gambiae have developed multi-resistance to insecticides, and to assess in experimental huts the performance of a mixed combination of pyrethroids and piperonyl butoxide (PBO) treated nets on these resistant mosquitoes.

Methods: The study was conducted at Kpomè, Southern Benin. The bio-efficacy of LLINs against An. funestus and An. gambiae was assessed using the World Health Organization (WHO) cone and tunnel tests. A released/recapture experiment following WHO procedures was conducted to compare the efficacy of conventional LLINs treated with pyrethroids only and LLINs with combinations of pyrethroids and PBO. Prior to huts trials, we confirmed the level of insecticide and PBO residues in tested nets using high performance liquid chromatography (HPLC).

Results: Conventional LLINs (Type 2 and Type 4) have the lowest effect against local multi-resistant An. funestus s.s. and An. coluzzii populations from Kpomè. Conversely, when LLINs containing mixtures of pyrethroids and PBO (Type 1 and Type 3) were introduced in trial huts, we recorded a greater effect against the two mosquito populations (P < 0.0001). Tunnel test with An. funestus s.s. revealed mortalities of over 80% with this new generation of LLINs (Type 1 and Type 3),while conventional LLINs produced 65.53 ± 8.33% mortalities for Type 2 and 71.25 ±7.92% mortalities for Type 4. Similarly, mortalities ranging from 77 to 87% were recorded with the local populations of An. coluzzii.

Conclusion: This study suggests the reduced efficacy of conventional LLINs (Pyrethroids alone) currently distributed in Benin communities where Anopheles populations have developed multi-insecticide resistance. The new generation nets (pyrethroids+PBO) proved to be more effective on multi-resistant populations of mosquitoes.

Original language	English
Article number	71
Journal	Wellcome Open Research
Volume	3
DOIs	https://doi.org/10.12688/wellcomeopenres.14589.1
Publication status	Published - 13 Jun 2018

Keywords

An. Coluzzii
An. Funestus s.s
LLINs
Multi-resistance controlling
PBO
Pyrethroids

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.12688/wellcomeopenres.14589.1Licence: CC BY

Wellcome open research experimental huts trail of the efficacy of pyrethroidsFinal published version, 649 KBLicence: CC BY

Cite this

Akoton, R., Tchigossou, G. M., Djègbè, I., Yessoufou, A., Atoyebi, M. S., Tossou, E., Zeukeng, F., Boko, P., Irving, H., Adéoti, R., Riveron, J., Wondji, C., Moutairou, K., & Djouaka, R. (2018). Experimental huts trial of the efficacy of pyrethroids/piperonyl butoxide (PBO) net treatments for controlling multi-resistant populations of Anopheles funestus s.s. in Kpomè, Southern Benin. Wellcome Open Research, 3, Article 71. https://doi.org/10.12688/wellcomeopenres.14589.1

@article{7a3c51c9c838458ebf10d54b9ae29f38,

title = "Experimental huts trial of the efficacy of pyrethroids/piperonyl butoxide (PBO) net treatments for controlling multi-resistant populations of Anopheles funestus s.s. in Kpom{\`e}, Southern Benin",

abstract = "Background: Insecticides resistance in Anopheles mosquitoes limits Long-Lasting Insecticidal Nets (LLIN) used for malaria control in Africa, especially Benin. This study aimed to evaluate the bio-efficacy of current LLINs in an area where An. funestus s.l. and An. gambiae have developed multi-resistance to insecticides, and to assess in experimental huts the performance of a mixed combination of pyrethroids and piperonyl butoxide (PBO) treated nets on these resistant mosquitoes.Methods: The study was conducted at Kpom{\`e}, Southern Benin. The bio-efficacy of LLINs against An. funestus and An. gambiae was assessed using the World Health Organization (WHO) cone and tunnel tests. A released/recapture experiment following WHO procedures was conducted to compare the efficacy of conventional LLINs treated with pyrethroids only and LLINs with combinations of pyrethroids and PBO. Prior to huts trials, we confirmed the level of insecticide and PBO residues in tested nets using high performance liquid chromatography (HPLC).Results: Conventional LLINs (Type 2 and Type 4) have the lowest effect against local multi-resistant An. funestus s.s. and An. coluzzii populations from Kpom{\`e}. Conversely, when LLINs containing mixtures of pyrethroids and PBO (Type 1 and Type 3) were introduced in trial huts, we recorded a greater effect against the two mosquito populations (P < 0.0001). Tunnel test with An. funestus s.s. revealed mortalities of over 80\% with this new generation of LLINs (Type 1 and Type 3),while conventional LLINs produced 65.53 ± 8.33\% mortalities for Type 2 and 71.25 ±7.92\% mortalities for Type 4. Similarly, mortalities ranging from 77 to 87\% were recorded with the local populations of An. coluzzii.Conclusion: This study suggests the reduced efficacy of conventional LLINs (Pyrethroids alone) currently distributed in Benin communities where Anopheles populations have developed multi-insecticide resistance. The new generation nets (pyrethroids+PBO) proved to be more effective on multi-resistant populations of mosquitoes.",

keywords = "An. Coluzzii, An. Funestus s.s, LLINs, Multi-resistance controlling, PBO, Pyrethroids",

author = "Romaric Akoton and Tchigossou, \{Genevieve M.\} and Innocent Dj{\`e}gb{\`e} and Akadiri Yessoufou and Atoyebi, \{Michael Seun\} and Eric Tossou and Francis Zeukeng and Pelagie Boko and Helen Irving and Razack Ad{\'e}oti and Jacob Riveron and Charles Wondji and Kabirou Moutairou and Rousseau Djouaka",

year = "2018",

month = jun,

day = "13",

doi = "10.12688/wellcomeopenres.14589.1",

language = "English",

volume = "3",

journal = "Wellcome Open Research",

issn = "2398-502X",

publisher = "F1000 Research Ltd",

}

Akoton, R, Tchigossou, GM, Djègbè, I, Yessoufou, A, Atoyebi, MS, Tossou, E, Zeukeng, F, Boko, P, Irving, H, Adéoti, R, Riveron, J, Wondji, C, Moutairou, K & Djouaka, R 2018, 'Experimental huts trial of the efficacy of pyrethroids/piperonyl butoxide (PBO) net treatments for controlling multi-resistant populations of Anopheles funestus s.s. in Kpomè, Southern Benin', Wellcome Open Research, vol. 3, 71. https://doi.org/10.12688/wellcomeopenres.14589.1

Experimental huts trial of the efficacy of pyrethroids/piperonyl butoxide (PBO) net treatments for controlling multi-resistant populations of Anopheles funestus s.s. in Kpomè, Southern Benin. / Akoton, Romaric; Tchigossou, Genevieve M.; Djègbè, Innocent et al.
In: Wellcome Open Research, Vol. 3, 71, 13.06.2018.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Experimental huts trial of the efficacy of pyrethroids/piperonyl butoxide (PBO) net treatments for controlling multi-resistant populations of Anopheles funestus s.s. in Kpomè, Southern Benin

AU - Akoton, Romaric

AU - Tchigossou, Genevieve M.

AU - Djègbè, Innocent

AU - Yessoufou, Akadiri

AU - Atoyebi, Michael Seun

AU - Tossou, Eric

AU - Zeukeng, Francis

AU - Boko, Pelagie

AU - Irving, Helen

AU - Adéoti, Razack

AU - Riveron, Jacob

AU - Wondji, Charles

AU - Moutairou, Kabirou

AU - Djouaka, Rousseau

PY - 2018/6/13

Y1 - 2018/6/13

N2 - Background: Insecticides resistance in Anopheles mosquitoes limits Long-Lasting Insecticidal Nets (LLIN) used for malaria control in Africa, especially Benin. This study aimed to evaluate the bio-efficacy of current LLINs in an area where An. funestus s.l. and An. gambiae have developed multi-resistance to insecticides, and to assess in experimental huts the performance of a mixed combination of pyrethroids and piperonyl butoxide (PBO) treated nets on these resistant mosquitoes.Methods: The study was conducted at Kpomè, Southern Benin. The bio-efficacy of LLINs against An. funestus and An. gambiae was assessed using the World Health Organization (WHO) cone and tunnel tests. A released/recapture experiment following WHO procedures was conducted to compare the efficacy of conventional LLINs treated with pyrethroids only and LLINs with combinations of pyrethroids and PBO. Prior to huts trials, we confirmed the level of insecticide and PBO residues in tested nets using high performance liquid chromatography (HPLC).Results: Conventional LLINs (Type 2 and Type 4) have the lowest effect against local multi-resistant An. funestus s.s. and An. coluzzii populations from Kpomè. Conversely, when LLINs containing mixtures of pyrethroids and PBO (Type 1 and Type 3) were introduced in trial huts, we recorded a greater effect against the two mosquito populations (P < 0.0001). Tunnel test with An. funestus s.s. revealed mortalities of over 80% with this new generation of LLINs (Type 1 and Type 3),while conventional LLINs produced 65.53 ± 8.33% mortalities for Type 2 and 71.25 ±7.92% mortalities for Type 4. Similarly, mortalities ranging from 77 to 87% were recorded with the local populations of An. coluzzii.Conclusion: This study suggests the reduced efficacy of conventional LLINs (Pyrethroids alone) currently distributed in Benin communities where Anopheles populations have developed multi-insecticide resistance. The new generation nets (pyrethroids+PBO) proved to be more effective on multi-resistant populations of mosquitoes.

AB - Background: Insecticides resistance in Anopheles mosquitoes limits Long-Lasting Insecticidal Nets (LLIN) used for malaria control in Africa, especially Benin. This study aimed to evaluate the bio-efficacy of current LLINs in an area where An. funestus s.l. and An. gambiae have developed multi-resistance to insecticides, and to assess in experimental huts the performance of a mixed combination of pyrethroids and piperonyl butoxide (PBO) treated nets on these resistant mosquitoes.Methods: The study was conducted at Kpomè, Southern Benin. The bio-efficacy of LLINs against An. funestus and An. gambiae was assessed using the World Health Organization (WHO) cone and tunnel tests. A released/recapture experiment following WHO procedures was conducted to compare the efficacy of conventional LLINs treated with pyrethroids only and LLINs with combinations of pyrethroids and PBO. Prior to huts trials, we confirmed the level of insecticide and PBO residues in tested nets using high performance liquid chromatography (HPLC).Results: Conventional LLINs (Type 2 and Type 4) have the lowest effect against local multi-resistant An. funestus s.s. and An. coluzzii populations from Kpomè. Conversely, when LLINs containing mixtures of pyrethroids and PBO (Type 1 and Type 3) were introduced in trial huts, we recorded a greater effect against the two mosquito populations (P < 0.0001). Tunnel test with An. funestus s.s. revealed mortalities of over 80% with this new generation of LLINs (Type 1 and Type 3),while conventional LLINs produced 65.53 ± 8.33% mortalities for Type 2 and 71.25 ±7.92% mortalities for Type 4. Similarly, mortalities ranging from 77 to 87% were recorded with the local populations of An. coluzzii.Conclusion: This study suggests the reduced efficacy of conventional LLINs (Pyrethroids alone) currently distributed in Benin communities where Anopheles populations have developed multi-insecticide resistance. The new generation nets (pyrethroids+PBO) proved to be more effective on multi-resistant populations of mosquitoes.

KW - An. Coluzzii

KW - An. Funestus s.s

KW - LLINs

KW - Multi-resistance controlling

KW - PBO

KW - Pyrethroids

U2 - 10.12688/wellcomeopenres.14589.1

DO - 10.12688/wellcomeopenres.14589.1

M3 - Article

SN - 2398-502X

VL - 3

JO - Wellcome Open Research

JF - Wellcome Open Research

M1 - 71

ER -

Experimental huts trial of the efficacy of pyrethroids/piperonyl butoxide (PBO) net treatments for controlling multi-resistant populations of Anopheles funestus s.s. in Kpomè, Southern Benin

Abstract

Keywords

UN SDGs

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