Abstract
High-efficiency transformation of DNA is integral to the study of mycobacteria, allowing genetic manipulation. Electroporation is the most widely used method for introducing DNA into mycobacterial strains. Many parameters contribute to high-efficiency transformation; these include the species per strain, the transforming DNA, the selectable marker, the growth medium additives, and the conditions of electroporation. In this chapter we provide an optimized method for the transformation of representative slowand fast-growing species of mycobacteria— Mycobacterium tuberculosis and M. smegmatis, respectively.
| Original language | English |
|---|---|
| Title of host publication | Mycobacteria Protocols |
| Publisher | Springer New York |
| Pages | 117-130 |
| Number of pages | 14 |
| Volume | 1285 |
| Edition | 3rd |
| ISBN (Electronic) | 978-1-4939-2450-9 |
| ISBN (Print) | 978-1-4939-2449-3 |
| DOIs | |
| Publication status | Published - 1 Jan 2015 |
| Externally published | Yes |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Publisher | Humana Press Inc. |
| ISSN (Print) | 1064-3745 |
UN SDGs
This output contributes to the following UN Sustainable Development Goals (SDGs)
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SDG 3 Good Health and Well-being
Keywords
- Electrocompetent cells
- Selectable marker
- Transformants
- Transforming DNA
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