Electron transfer in human cytochrome P450 reductase

A. Gutierrez; A. Grunau; Mark Paine; A. W. Munro; C. R. Wolf; G. C.K. Roberts; N. S. Scrutton

doi:10.1042/bst0310497

Electron transfer in human cytochrome P450 reductase

A. Gutierrez, A. Grunau, Mark Paine, A. W. Munro, C. R. Wolf, G. C.K. Roberts, N. S. Scrutton

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68 Citations (Scopus)

Abstract

Cytochrome P450 reductase (CPR) is a diflavin enzyme responsible for electron donation to mammalian cytochrome P450 enzymes in the endoplasmic reticulum. Dissection of the enzyme into functional domains and studies by site-directed mutagenesis have enabled detailed characterization of the mechanism of electron transfer using stopped-flow and equilibrium-perturbation methods, and redox potentiometry. These studies and the mechanism of electron transfer in CPR are reported herein.

Original language	English
Pages (from-to)	497-501
Number of pages	5
Journal	Biochemical Society Transactions
Volume	31
Issue number	3
DOIs	https://doi.org/10.1042/bst0310497
Publication status	Published - 1 Jun 2003
Externally published	Yes

Keywords

Cytochrome P450 reductase
Electron transfer
Flavoprotein
Kinetics

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10.1042/bst0310497

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title = "Electron transfer in human cytochrome P450 reductase",

abstract = "Cytochrome P450 reductase (CPR) is a diflavin enzyme responsible for electron donation to mammalian cytochrome P450 enzymes in the endoplasmic reticulum. Dissection of the enzyme into functional domains and studies by site-directed mutagenesis have enabled detailed characterization of the mechanism of electron transfer using stopped-flow and equilibrium-perturbation methods, and redox potentiometry. These studies and the mechanism of electron transfer in CPR are reported herein.",

keywords = "Cytochrome P450 reductase, Electron transfer, Flavoprotein, Kinetics",

author = "A. Gutierrez and A. Grunau and Mark Paine and Munro, \{A. W.\} and Wolf, \{C. R.\} and Roberts, \{G. C.K.\} and Scrutton, \{N. S.\}",

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language = "English",

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T1 - Electron transfer in human cytochrome P450 reductase

AU - Gutierrez, A.

AU - Grunau, A.

AU - Paine, Mark

AU - Munro, A. W.

AU - Wolf, C. R.

AU - Roberts, G. C.K.

AU - Scrutton, N. S.

PY - 2003/6/1

Y1 - 2003/6/1

N2 - Cytochrome P450 reductase (CPR) is a diflavin enzyme responsible for electron donation to mammalian cytochrome P450 enzymes in the endoplasmic reticulum. Dissection of the enzyme into functional domains and studies by site-directed mutagenesis have enabled detailed characterization of the mechanism of electron transfer using stopped-flow and equilibrium-perturbation methods, and redox potentiometry. These studies and the mechanism of electron transfer in CPR are reported herein.

AB - Cytochrome P450 reductase (CPR) is a diflavin enzyme responsible for electron donation to mammalian cytochrome P450 enzymes in the endoplasmic reticulum. Dissection of the enzyme into functional domains and studies by site-directed mutagenesis have enabled detailed characterization of the mechanism of electron transfer using stopped-flow and equilibrium-perturbation methods, and redox potentiometry. These studies and the mechanism of electron transfer in CPR are reported herein.

KW - Cytochrome P450 reductase

KW - Electron transfer

KW - Flavoprotein

KW - Kinetics

U2 - 10.1042/bst0310497

DO - 10.1042/bst0310497

M3 - Article

SN - 0300-5127

VL - 31

SP - 497

EP - 501

JO - Biochemical Society Transactions

JF - Biochemical Society Transactions

IS - 3

ER -

Electron transfer in human cytochrome P450 reductase

Abstract

Keywords

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